The effect of estrone and estradiol on the expression of the orexin/hypocretin system in the porcine uterus during early pregnancy

Kieżun, Marta; Dobrzyń, Kamil; Rytelewska, Edyta; Kisielewska, Katarzyna; Gudelska, Marlena; Szeszko, Karol; Zaobidna, Ewa; Bors, Kinga; Wyrębek, Joanna; Mykytiuk, A.; Kamiński, Tadeusz

doi:10.1016/j.domaniend.2018.12.008

Cited by 9 publications

(8 citation statements)

References 34 publications

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“…Only the secretion of OXA increased under the influence of PGs. In our previous studies, a similar pattern of orexin system expression was observed in the endometrium under the influence of P 4 and oestrogens (Dobrzyn et al, 2018;Kiezun et al, 2019). These findings suggest that similarly to steroids, PGs can decrease uterine sensitivity to OXs during this period of gestation to maintain high production of steroids and PGs in the endometrium.…”

Section: Discussionsupporting

confidence: 74%

“…Although the regulation of orexin system expression in various tissues has been extensively examined, little is known about the factors that can affect this process in the uterus. Our latest studies have demonstrated that both progesterone (P 4 ) and oestrogens (E 1 and E 2 ) can act as regulators of orexin system expression, at least in the porcine uterus during early pregnancy and the oestrous cycle (Dobrzyn et al, 2018;Kiezun et al, 2019). However, we hypothesised that other key regulators of female reproduction may also affect orexin system expression in the uterus.…”

Section: Discussionmentioning

confidence: 96%

“…After blocking, the membranes were incubated for another 12 h at the same temperature with rabbit polyclonal anti-OX1R (1:300; ab68718, Abcam, UK), anti-OX2R (1:150; ab104701, Abcam, UK) or antiactin (1:150; A2066, Sigma-Aldrich, USA) antibodies, that were used to quantify porcine OX1R and OX2R proteins abundance. The antibodies against OX1R and OX2R had been successfully used in our earlier studies (Dobrzyn et al, 2018;Kiezun et al, 2019). To identify the immunoreactive products, the membranes were incubated for 2 h at room temperature with goat anti-rabbit IgG conjugated with alkaline phosphatase (sc-2057, Santa Cruz Biotechnology, USA) for OX1R and OX2R (1:500), and actin (1:2000).…”

Section: Western Blotmentioning

confidence: 99%

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The effect of prostaglandins E₂ and F_2α on orexin system expression in the porcine uterus during the peri-implantation period

Kieżun

Dobrzyń

Gudelska

et al. 2022

Annals of Animal Science

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The aim of this study was to evaluate the effect of prostaglandins E2 (PGE2) and F2α (PGF2α) on orexins (OXA and OXB) secretion (ELISA), prepro-orexin (PPO) gene expression and the content of orexin receptors (OX1R, OX2R) mRNA (qPCR) and proteins (Western blot) in porcine endometrial and myometrial tissue slices during early pregnancy (days 10–28) and on days 10–11 of the oestrous cycle. On days 10–11 of pregnancy, prostaglandins (PGs) decreased the expression of the PPO gene and OXR gene and protein in the endometrium. On days 12– 13, PGs increased OXB secretion, PGE2 enhanced OXA secretion, and PGF2α suppressed PPO expression. On days 15–16, both PGs inhibited PPO expression and OXB secretion, and PGF2α increased OXA release. On days 27–28, PGs decreased PPO mRNA and OX1R protein levels, PGE2 decreased OX2R protein content, and PGF2α decreased OXB secretion. On days 10-11 of the cycle PGs increased the expression of PPO mRNA and OX2R protein, whereas PGE2 increased OX1R protein content. The effect of PGs on orexin system expression in the porcine myometrium was dependent on the animal physiological status and the concentrations of specific PGs. The study demonstrated that PGs exert regulatory effects on orexin system expression in the porcine uterus, which suggests that a local regulatory mechanism could be responsible for modulating uterine metabolism. The observed differences in PGs’ influence on orexin system expression could be attributed to changes in the sensitivity of the studied tissues, associated with the phase of the oestrous cycle, the stage of early pregnancy or PGs concentration.

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Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 96%

Section: Western Blotmentioning

confidence: 99%

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The effect of prostaglandins E₂ and F_2α on orexin system expression in the porcine uterus during the peri-implantation period

Kieżun

Dobrzyń

Gudelska

et al. 2022

Annals of Animal Science

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“…Most authors focus on the role of OXA, which is regarded as a more important and active hormone than OXB. However, our earlier studies (Kiezun et al., 2019; Smolinska et al., 2017) demonstrated that during early pregnancy, the concentrations of OXB in porcine plasma and uterine luminal fluid, as well as in culture media after culturing porcine endometrial slices exceeded OXA levels. The above could suggest that despite its lower activity and affinity for orexin receptors, OXB could play an equally important role to OXA in the reproductive tract due to the higher concentrations of this hormone in the uterine environment.…”

Section: Discussionmentioning

confidence: 85%

Orexin B affects the transcriptome of incubated in vitro porcine endometrial explants from the early‐implantation period

Dobrzyń

Kieżun

Szeszko

et al. 2020

Reprod Domestic Animals

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This study determined the effect of orexin B (OXB) on the porcine endometrial transcriptome during the embryo attachment phase. Microarray analyses of gene ontology (GO), biological pathways, networks and differentially expressed genes (DEG) were performed. Orexin B influenced the expression of 887 genes (fold change > 1.2; p < .05): 620 genes were up‐regulated, and 267 were down‐regulated. The analysis of the relationship between DEG revealed that OXB interacts with genes linked with processes such as cell hormone binding, regulation of hormone levels, lipid transport, steroid metabolic processes, the apoptotic signalling pathway and the acute inflammatory response, which are pivotal for reproductive success. Orexin B played a bivalent role in the early‐pregnant uterus by limiting the pregnancy outcome, promoting embryo development, suppressing the immune system and, consequently, preventing embryo rejection. These findings suggest that OXB could be responsible for the proper course of gestation by adapting litter size to the metabolic status of the maternal organism.

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“…P4 can stimulate the production and release of uterine secretions in early pregnancy, thus regulating pregnancy [ 54 ]. Oestrogen is a key hormone that controls maternal reproductive function, pregnancy establishment and early embryonic implantation [ 55 ]. Serum levels of oestrogen and progesterone are significantly increased in mice treated with antioxidants in early pregnancy [ 56 ].…”

Section: Discussionmentioning

confidence: 99%

A Proteomic Study of the Effect of N-acetylcysteine on the Regulation of Early Pregnancy in Goats

Yang

Chen

Tian

et al. 2022

Animals

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Dietary supplementation with N-acetyl-L-cysteine (NAC) may support early pregnancy regulation and fertility in female animals. The purpose of this study was to investigate the effect of supplementation with 0.07% NAC on the expression of the uterine keratin gene and protein in Qianbei-pockmarked goats during early pregnancy using tandem mass spectrometry (TMT) relative quantitative proteomics. The results showed that there were significant differences in uterine keratin expression between the experimental group (NAC group) and the control group on day 35 of gestation. A total of 6271 proteins were identified, 6258 of which were quantified by mass spectrometry. There were 125 differentially expressed proteins (DEPs), including 47 upregulated and 78 downregulated proteins, in the NAC group. Bioinformatic analysis showed that these DEPs were mainly involved in the transport and biosynthesis of organic matter and were related to the binding of transition metal ions, DNA and proteins and the catalytic activity of enzymes. They were enriched in the Jak-STAT signalling pathway, RNA monitoring pathway, amino acid biosynthesis, steroid biosynthesis and other pathways that may affect the early pregnancy status of does through different pathways and thus influence early embryonic development. Immunohistochemistry, real-time quantitative PCR and Western blotting were used to verify the expression and localization of glial fibrillary acidic protein (GFAP) and pelota mRNA surveillance and ribosomal rescue factor (PELO) in uterine horn tissue. The results showed that both PELO and GFAP were localized to endometrial and stromal cells, consistent with the mass spectrometry data at the transcriptional and translational levels. Moreover, NAC supplementation increased the levels of the reproductive hormones follicle-stimulating hormone (FSH), luteinizing hormone (LH), oestradiol (E2), progesterone (P4), superoxide dismutase (SOD), glutamate peroxidase (GSH-Px) and nitric oxide (NO) in the serum of does. These findings provide new insight into the mechanism by which NAC regulates early pregnancy and embryonic development in goats.

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The effect of estrone and estradiol on the expression of the orexin/hypocretin system in the porcine uterus during early pregnancy

Cited by 9 publications

References 34 publications

The effect of prostaglandins E₂ and F_2α on orexin system expression in the porcine uterus during the peri-implantation period

The effect of prostaglandins E₂ and F_2α on orexin system expression in the porcine uterus during the peri-implantation period

Orexin B affects the transcriptome of incubated in vitro porcine endometrial explants from the early‐implantation period

A Proteomic Study of the Effect of N-acetylcysteine on the Regulation of Early Pregnancy in Goats

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The effect of estrone and estradiol on the expression of the orexin/hypocretin system in the porcine uterus during early pregnancy

Cited by 9 publications

References 34 publications

The effect of prostaglandins E2 and F2α on orexin system expression in the porcine uterus during the peri-implantation period

The effect of prostaglandins E2 and F2α on orexin system expression in the porcine uterus during the peri-implantation period

Orexin B affects the transcriptome of incubated in vitro porcine endometrial explants from the early‐implantation period

A Proteomic Study of the Effect of N-acetylcysteine on the Regulation of Early Pregnancy in Goats

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The effect of prostaglandins E₂ and F_2α on orexin system expression in the porcine uterus during the peri-implantation period

The effect of prostaglandins E₂ and F_2α on orexin system expression in the porcine uterus during the peri-implantation period