The Effect of Essential Oil Intake on Changes of Plasma Antioxidant Status in Mice

Abstract

Faix Š., Š. Juhas, Z. Faixová: The Effect of Essential Oil Intake on Changes of Plasma Antioxidant Status in Mice. Acta Vet. Brno 2007, 76: 357-361.The aim of this study was to determine the effects of four essential oils intake by feed, namely Origanum vulgare, Thymus vulgaris, Cinnamomum zeylanicum Ness, and Syzygium aromaticum on antioxidant status in mice in vivo. Essential oils were in the aether oleum form. They were diluted with ethanol absolute mixed with ground pelett (0.1, 0.25, 0.57 and 1% concentration) and thereafter ethanol was evaporated. SOD, GPx activities and TAS were measured in erythrocytes and plasma spectrophotometrically with Ransod, Ransel and TAS kits from RANDOX, respectively. GPX activity showed a signifi cant increase in 0.25% and 0.1% concentration of Origani aetheroleum. The GPx activities were decreased in 1% concentration of Thymi aetheroleum and 0.57% concentration of Cinnamomi aetheroleum and 0.57% concentration of Caryophylli aetheroleum. The total antioxidant status showed a signifi cant decrease in 1 % concentration of Origani aetheroleum and signifi cantly increased in 0.1% concentration. The same results were found in Thymi aetheroleum. Cinnamomi aetheroleum and Caryophylli aetheroleum had not effect on total antioxidant status. SOD activities were not signifi cantly changed after intake of essential oils. In conclusion, our results showed, that concentration of essential oil is very important for antioxidant status and also for metabolism of mice, because a high dose of essential oil has adverse effect on metabolism of mice, representated by a lower growth of the body weight. On the other hand, essential oils at lower concentrations have positive effect on antioxidant status of mice.

A Study Medicinal and Nutritional Effects of Thyme on Hematological Changes in Male Albino Rats

“…Thyme contain Rosmarinic acid which acts as an inhibitor that inhibition syntheis of Eosinphils, this result agree with [28].…”

“…And disagree of the present study with [28] when they found that high dose of origanum vulgare oil had adverse effects on metabolism of mice on the contrary to the lower concentration which have appositive effects on antioxidant status. ct of phenol in olive (200 mg/kg) effect on twenty rats for 30 days, the result increase in count of lymphocytes and monocyte, the exposure to phenol component leading to inflammation , and this reaction characterized by movement of lymphocytes and monocytes from blood to the extravascular tissue this led to increase in count of lymphocytes and monocytes, Therefore, monocytes increased in circulation and phagocytosis increased in tissue, to prevent extensive damage to the body and disagree with [26] The decreases in the lymphocytes by thyme affect the effector cells of the immune system due to inhibit the body's immunity.…”

Effect of Cinnamomum zeylanicum Essential Oil on Antioxidative Status in Broiler Chickens

“…SOD and CAT were not significantly affected in chickens fed diet supplemented with Cinnamomum zeylanicum essential oil. It was observed that feeding diets containing different doses of essential oils affected the GPx activity in the blood of mice (Faix et al 2007). Differences in antioxidant activities of cinnamon might arise from different parts of plant.…”

Effect of dietary Salvia officinalis essential oil and sodium selenite supplementation on antioxidative status and blood phagocytic activity in broiler chickens

“…Measurements of plasma antioxidants intended to assess the total antioxidant status in vivo showed higher values in broilers fed the diet supplemented with Salvia officinalis essential oil in our study. Faix et al (2007) found 46 The combined treatment of Salvia officinalis essential oil and sodium selenite caused significantly lower activity of SOD in blood erythrocytes compared to that of broilers fed a basal diet or supplemented with sodium selenite only. L i n et al (2000) reported that polyphenols proved to be effective superoxide radical scavengers.…”

“…In recent years, essential oils and extracts of many plants have been studied for their antioxidant activities (Faix et al 2007(Faix et al , 2009Hussain et al 2011). Salvia officinalis is a very popular aromatic plant well known for its wide range of medicinal effects.…”