The Effect of Erythropoietin on Testosterone Levels During Ischemia Reperfusion Injury in Rats

Abstract

Objective: This experimental study examined the effect of erythropoietin (Epo) in a rat model and particularly in an adrenal ischemia-reperfusion (IR) protocol. The effect of that molecule was studied biochemically using blood mean testosterone levels (T). Materials and methods: 40 rats of mean weight 247.7 g were used in the study. T levels were measured at 60 min (groups A and C) and at 120 min (groups B and D) of reperfusion. Erythropoietin was administered only in groups C and D. Results: Erythropoietin administration non significantly increased the testosterone levels by 71.21%+44.19% (p=0.1080). Reperfusion time non-significantly decreased the testosterone levels by 65.17%+44.45% (p=0.0792). However, erythropoietin administration and reperfusion time together produced a non-significant combined effect in increasing the testosterone levels by 27.65%+27.21% (p= 0.3006). Conclusions: Erythropoietin administration whether it interacted or not with reperfusion time has increasing non significant short-term effects on testosterone levels. Perhaps, a longer study time or a higher Epo dose, may reveal clearer and more significant effects.

The Acute Effect of Erythropoietin on Glucose Levels During Ischemia Reperfusion Injury in Rats

“…The detailed anesthesiologic technique is described in related references (Tsompos et al 2015a, Tsompos et al 2015b. Oxygen supply, electrocardiogram and acidometry were continuously provided during whole experiment performance.…”

“…However, just few relative reports were found concerning Epo trial in IR experiments, not covering completely this particular matter. A meta-analysis of 13 published seric variables, coming from the same experimental setting, tried to provide a numeric evaluation of the Epo efficacy at the same endpoints (Table 1) (Tsompos et al 2015a, Tsompos et al 2015b. Furthermore, several publications addressed trials of other similar molecules of growth factors to which the studied molecule also belongs to (Bader et al 2011, Wang et al 2011.…”