The effect of environmental parameters on growth, cholinesterase activity and glutathione S-transferase activity in the earthworm (Apporectodea caliginosa)

Booth, Lynn; Heppelthwaite, V. J.; Mcglinchy, Aaron

doi:10.1080/135475000230532

Cited by 46 publications

(26 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[54] [55] [56]. Earthworm, Lumbricus rubellus, possess a range of GSTs related to those from other taxa like nematodes and humans, with the evidence of tissue specific isoforms, activity, location, the ability to detoxify products of cellular toxicity and potential response to pollution [57].…”

Section: Glutathione-s-transferase (Gst)mentioning

confidence: 99%

Enzymes of Earthworm as Indicators of Pesticide Pollution in Soil

Tiwari¹,

Singh²,

Pandey³

et al. 2016

AER

View full text Add to dashboard Cite

The importance of the earthworms in the agricultural practices is well known. The increasing applications of pesticides and chemicals in the agricultural farms have adversely influenced the flora and fauna of the soil. Earthworms which immensely contribute in increasing the quality and fertility of agricultural soil are reported be worst hit organisms under such conditions. Recent reports have indicated growing interests among researchers to explore biochemical and molecular markers as indicators of accumulation of pollutants in the soil in general and pesticides in particular. The varying levels of several biomolecules in different parts of the earthworm have been reported which are indicative of sensitivity of the organisms to different xenobiotics. However, the existing information lacks the literature displaying stock of information regarding the impact of pesticides on the levels of some key enzymes regulating many crucial functions in the earthworm at one place. Keeping in view this issue, it was envisaged to bring out a mini review which illustrates updated information available on the impact of pesticides on the activities of certain key enzymes reported to be responsible for catalysing metabolic pathways concerning the neurotransmission system, energy metabolism, oxidative stress and amino acids metabolism in different body parts of the earthworms, a prospective bioindicators of pesticides contamination in the soil.

show abstract

Section: Glutathione-s-transferase (Gst)mentioning

confidence: 99%

Enzymes of Earthworm as Indicators of Pesticide Pollution in Soil

Tiwari¹,

Singh²,

Pandey³

et al. 2016

AER

View full text Add to dashboard Cite

show abstract

“…Booth, Heppelthwaite and Mc Glinchy (2000) reported the existence of the glutathione (GSH) and glutathione-S-transferase (GST) system in earthworms. Saint-Denis, Narbonne, Arnaud, and Ribera (2001) studied the activities of enzymes (catalase, glutathione peroxidase, glutathione-S-transferase and glutathione reductase) involved in antioxidant defence systems in Eisenia fetida, which are mainly localized in the cytosolic fractions.…”

mentioning

confidence: 99%

Glutathione-S-Transferase Production in Earthworm as Tool for Assessment of Heavy Metal Pollution in Abattoir Soil

Owagboriaye

Dedeke

Ademolu

2016

RBT

View full text Add to dashboard Cite

Abstract:The use of direct response of animals to environmental challenges by production of biomarkers is a better tool to assess environmental pollution than the conventional methods. This study aimed to measure Glutathione-S-transferase (GST) in earthworms as tools for assessing heavy metal pollution in abattoir soil. Five (5) replicates each of earthworm species (Libyodrilus violaceous, Eudrilus eugeniae and Alma millsoni), soil and rumen waste samples were collected from three (3) abattoir sites (Lafenwa, Gbonogun and Madojutimi abattoirs), and a control site located within Federal University of Agriculture Abeokuta, beside an undisturbed stream with no rumen waste. Heavy metal (Cu, Zn, Pb, Cd, Co, Cr, Ni and Mn) concentrations in rumen waste, abattoir soils and earthworm tissues were determined using Atomic Absorption Spectrophotometer. The pH and organic matter (OM) concentrations of the rumen waste and abattoir soils were determined by standard methods. GST activities in the earthworm tissues were determined through the conjugation of 1 mM reduced glutathione (GSH) with 1 mM 1-chloro-2,4-dinitrobenzene (CDNB). The rumen waste recorded significantly higher (p ≤ 0.05) % OM, heavy metal concentrations and pH level than in their respective abattoir soils. The mean heavy metal concentrations of Cu, Zn, Pb, Cd and Mn were highest in the tissue of earthworm species obtained from Lafenwa abattoir. A significantly (p ≤ 0.05) higher GST activities were recorded in the tissue of earthworm species obtained from Lafenwa and Gbonogun abattoirs. Libyodrilous violaceus obtained from Lafenwa abattoir recorded the highest GST activity (8.47±1.39) in their tissue followed by the ones from Gbonogun abattoir (8.21±0.85). A significant (p ≤ 0.05) positive correlations was observed between GST activities in earthworm tissues and heavy metal concentrations. GST activities can therefore be used to assess the level of heavy metal pollution in abattoir soils. Rev. Biol. Trop. 64 (2): 779-789. Epub 2016 June 01.

show abstract

“…Besides, as has been indicated, carbamate pesticide along with organophosphates, strongly inhibits acetylcholinesterase (AChE) activity in a number of invertebrate species [22]. In earthworm Eisenia foetida this enzyme responsible for stability of lysosomal membrane of coelomocytes [23] and activity of muscle cells in the clitellum [24].…”

Section: Resultsmentioning

confidence: 99%