The effect of enterohemorrhagic Escherichia coli 0157:H7 on intestinal structure and solute transport in rabbits

Li, Zhe; Bell, Catherine; Buret, André G.; Robins-Browne, Roy M.; Stiel, Daniel; O’Loughlin, Edward

doi:10.1016/0016-5085(93)90415-9

Cited by 55 publications

(44 citation statements)

References 27 publications

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“…A number of experimental animal models have been proposed to study the pathogenicity of STEC (63), including gnotobiotic mice (17,18,64), streptomycin-treated mice (15,27,32,61,62), gnotobiotic piglets (14,59), newborn chickens (5), and infant rabbits (26,36,44,50). While mouse models have been used most frequently to date, most are not appropriate because high doses of inoculum, more than 10 7 CFU/body, are usually required for establishment of STEC infection, and often mice do not develop diarrhea even when they are inoculated with such high doses of STEC (21,23).…”

Section: Discussionmentioning

confidence: 99%

“…While mouse models have been used most frequently to date, most are not appropriate because high doses of inoculum, more than 10 7 CFU/body, are usually required for establishment of STEC infection, and often mice do not develop diarrhea even when they are inoculated with such high doses of STEC (21,23). On the contrary, the rabbit model has the major advantages of the reproducibility of diarrhea and susceptibility to STEC infection and Stx toxicity (7,26,36,44,50,55). In this study, we used the infant rabbit model with administration of a lower number of bacteria (ϳ10 3 CFU/body) than reported previously (36,44,50).…”

Section: Discussionmentioning

confidence: 99%

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Protective Effect ofLactobacillus caseiStrain Shirota on Shiga Toxin-ProducingEscherichia coliO157:H7 Infection in Infant Rabbits

et al. 2001

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Protective Effect ofLactobacillus caseiStrain Shirota on Shiga Toxin-ProducingEscherichia coliO157:H7 Infection in Infant Rabbits

et al. 2001

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“…to result in less severe lesions in colonic epithelial cells than the E. coli containing the both Stx genes and a LEE region (15), thus suggesting that the LEE-induced A/E phenomenon and the following internalization play a crucial role in the pathogenesis of STEC infection.…”

mentioning

confidence: 99%

Role of Internalization in the Pathogenicity of Shiga Toxin‐Producing Escherichia coli Infection in a Gnotobiotic Murine Model

Aiba

Ishikawa

Shimizu

et al. 2002

Microbiology and Immunology

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We investigated the role of bacterial internalization in the killing caused by Shiga toxin‐producing Escherichia coli (STEC) infection using a gnotobiotic murine model. A high number of internalized STEC was found in the colonic epithelial cells of STEC‐infected mice by both an ex vivo assay and transmission electron microscopy. Most of these mice were killed within 10 days after infection. However, the implantation of lactic acid bacteria in such mice before infection markedly decreased the number of internalized STECs and also completely protected these hosts from killing by a STEC infection. The inhibition of such internalization by immunoglobulin also prevented the hosts from being killed. The Shiga toxin levels in these hosts indicated an inhibition of the penetration of Shiga toxins produced in the colon to the underlying tissue. These results suggested that the internalization plays an important role in the pathogenicity caused by STEC infection in a gnotobiotic murine model.

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“…Subsequent studies identified the colon as the principal site of EHEC-mediated disease, where histological abnormalities, including edema, hemorrhage, the presence of an inflammatory infiltrate, and mucosal epithelial apoptosis, were observed (66,67). In these young animals, the development of diarrhea appears to be driven by inflammation-mediated changes in colonic ion transport resulting in decreased Na absorption and increased Cl secretion (68,69). Unlike humans, suckling rabbits do not develop signs of renal disease; however, the reproduction of diarrhea and colonic inflammation, two key features of EHEC infection in humans, indicates that they may be a useful model for studying the intestinal manifestations of the disease.…”

Section: Rabbitsmentioning

confidence: 99%

Animal Models of EnterohemorrhagicEscherichia coliInfection

Ritchie

2014

Microbiol Spectr

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The first major outbreaks caused by enterohemorrhagic Escherichia coli (EHEC) raised public and medical awareness of the risks associated with acquiring this potentially deadly infection. The widespread presence of these organisms in the environment, the severity of the clinical sequelae, and the lack of treatment options and effective preventive measures demand that we obtain a better understanding of how this group of organisms cause disease. Animal models allow study of the processes and factors that contribute to disease and, as such, form a valuable tool in the repertoire of infectious disease researchers. Yet despite more than 30 years of research, it seems that no single model host reproduces the full spectrum of clinical disease induced by EHEC in humans. In the first part of this review, a synopsis of what is known about EHEC infections is garnered from human outbreaks and biopsy specimens. The main features and limitations of EHEC infection models that are based on the three most commonly used species (pigs, rabbits, and mice) are described within a historical context. Recent advances are highlighted, and a brief overview of models based on other species is given. Finally, the impact of the host on moderating EHEC infection is considered in light of growing evidence for the need to consider the biology and virulence strategies of EHEC in the context of its niche within the intestine.

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The effect of enterohemorrhagic Escherichia coli 0157:H7 on intestinal structure and solute transport in rabbits

Cited by 55 publications

References 27 publications

Protective Effect ofLactobacillus caseiStrain Shirota on Shiga Toxin-ProducingEscherichia coliO157:H7 Infection in Infant Rabbits

Protective Effect ofLactobacillus caseiStrain Shirota on Shiga Toxin-ProducingEscherichia coliO157:H7 Infection in Infant Rabbits

Role of Internalization in the Pathogenicity of Shiga Toxin‐Producing Escherichia coli Infection in a Gnotobiotic Murine Model

Animal Models of EnterohemorrhagicEscherichia coliInfection

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