The effect of endostatin evaluated in an experimental animal model of collagen‐induced arthritis

Kurosaka, Daitaro; Yoshida, Ken; Yasuda, Jun; Yasuda, Chiho; Noda, Kentaro; Furuya, Keizo; Ukichi, Taro; Kingetsu, Isamu; Joh, Kensuke; Yamaguchi, Noriko; Sakai, Saburo; Yamada, Akio

doi:10.1080/03009740701605913

Cited by 19 publications

(14 citation statements)

References 28 publications

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“…Age-matched 8–10-week-old control and S100A9−/− mice were immunized with adjuvant (complete Freund's on day 0 and incomplete Freund's on day 21)+100 mg bovine type II collagen in a 0.1 mL 1:1 v/v mixture of adjuvant+collagen injected subcutaneously into the base of the tail, similar to previously published reports (3, 11). Mice were assessed by a blinded observer for paw swelling and clinical disease until experiment termination when hindlimbs were processed for histopathology, as described above.…”

Section: Methodsmentioning

confidence: 87%

S100A9 is not essential for disease expression in an acute (K/BxN) or chronic (CIA) model of inflammatory arthritis

Rampersad

Esserman

McGinnis

et al. 2009

Scandinavian Journal of Rheumatology

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Objective: S100A8 (calgranulin A, MRP8) and S100A9 (calgranulin B, MRP14) are calcium-binding proteins highly expressed by activated myeloid cells and thought to be involved in the pathogenesis of inflammatory diseases. Circulating levels of S100A8/S100A9 are elevated in both human and experimental models of autoimmune disease, including rheumatoid arthritis (RA). Methods: Mice deficient in S100A9 (S100A9−/−) and wild-type controls were immunized using standard techniques for the K/BxN serum transfer or the collagen-induced arthritis (CIA) model. Results: S100A9−/− animals, with defective expression of both S100A8 and S100A9 proteins, had similar arthritis and histopathology to that of wild-type controls in both mouse models. Conclusion: S100A8 and S100A9 are not essential for disease expression in either the K/BxN serum transfer or the CIA model of inflammatory arthritis.

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Section: Methodsmentioning

confidence: 87%

S100A9 is not essential for disease expression in an acute (K/BxN) or chronic (CIA) model of inflammatory arthritis

Rampersad

Esserman

McGinnis

et al. 2009

Scandinavian Journal of Rheumatology

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“…In RA, VEGF has been reported to be present in the synovial fluid and serum of RA patients, and closely involved in arthritis pathogenesis, particularly angiogenesis [10][11][12]. Therefore, in this study, we examined the relationship between the degree of blood flow signals in the synovium and serum VEGF levels.…”

Section: Introductionmentioning

confidence: 99%

Correlation between synovial blood flow signals and serum vascular endothelial growth factor levels in patients with refractory rheumatoid arthritis

et al. 2009

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The objective of the study is to examine the relationship between synovial blood flow signals and vascular endothelial growth factor (VEGF) involved in angiogenesis by Doppler ultrasound. Twenty-one patients meeting the diagnostic criteria of the American College of Rheumatology (ACR) were enrolled in this study. Doppler ultrasound signals of blood flow in the wrist synovial membrane were measured and classified into three grades: grade 1 = no flow; grade 2 = mild flow; grade 3 = intense flow. A significant correlation was observed between blood flow signals in the wrist synovial membrane and serum VEGF levels (r = 0.5681, P = 0.0072). These results suggest that the measurement of Doppler ultrasound signals of blood flow in the wrist synovial membrane is useful in the evaluation of angiogenesis.

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“…Thus, the use of osmotic minipumps is not linked solely to the SCID mouse model, but could also be applied to other animal models such as CIA or AIA. Indeed, Kurosaka and coworkers used this device for the administration of endostatin in CIA induced arthritis in mice 24. In this study, the continously administration of endostatin using osmotic pumps over 14 days and a daily therapeutic administration resulted in inhibition of CIA in mice.…”

Section: Discussionmentioning

confidence: 88%

The therapeutic use of osmotic minipumps in the severe combined immunodeficiency (SCID) mouse model for rheumatoid arthritis

et al. 2008

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As the production of viruses coding for bioactive proteins is cost-and time-intensive, we established an in vivo long-term release model using osmotic minipumps in the SCID mouse model for RA. METHODS: Isolated RASF were cultured for 4 passages and coimplanted together with human cartilage and an Alzet(R) Osmotic Miniature Pump Model 2004 containing 200 microl of IL-10 and IL-1ra for 40 days in SCID mice. Implants were removed after 40 days and evaluated histologically. The actual rates of IL-10 and IL-1ra in murine serum were measured by ELISA. RESULTS: Release of IL-10 and IL-1ra by the pumps was effective as both could be measured in significant amounts in the serum of the mice. IL-10 and IL-1ra release showed protective effects towards the co-implanted cartilage, similar to the adenovirally IL-10-/IL-1ra-transduced RASF. The invasion scores for the implants with the osmotic pumps were: invasion 0.7+/-0.5, degradation 0.5+/-0.3 (all parameters significant vs. controls, p<0.05). CONCLUSIONS: The results demonstrate that the combination of osmotic pumps with the SCID mouse model for RA can be used as approach for application and evaluation of cartilage-protective molecules. Furthermore, the effect of cartilage-protective cytokines is independent of the type of application.

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The effect of endostatin evaluated in an experimental animal model of collagen‐induced arthritis

Abstract: Both prophylactic and therapeutic administration of endostatin inhibited type II CIA in mice. The administration method using an osmotic pump is useful.

Cited by 19 publications

References 28 publications

S100A9 is not essential for disease expression in an acute (K/BxN) or chronic (CIA) model of inflammatory arthritis

S100A9 is not essential for disease expression in an acute (K/BxN) or chronic (CIA) model of inflammatory arthritis

Correlation between synovial blood flow signals and serum vascular endothelial growth factor levels in patients with refractory rheumatoid arthritis

The therapeutic use of osmotic minipumps in the severe combined immunodeficiency (SCID) mouse model for rheumatoid arthritis

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