The effect of electromagnetic field on reactive oxygen species production in human neutrophils<i>in vitro</i>

Poniedziałek, Barbara; Rzymski, Piotr; Nawrocka-Bogusz, Honorata; Jaroszyk, Feliks; Wiktorowicz, Krzysztof

doi:10.3109/15368378.2012.721845

Cited by 29 publications

(22 citation statements)

References 40 publications

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“…Oxidative stress with free radical production is one of the proposed mechanisms by which LF EMF exposure affects cellular behaviour [4,13]. Neutrophils exposed to 50-Hz EMFs showed increased ROS and free radical production [13]. We used DPI to investigate if LF EMF exposure influenced NET formation in an NADPH-dependent manner (fig.…”

Section: Resultsmentioning

confidence: 99%

“…In addition, pharmacological inhibition of NADPH oxidase activity by DPI in healthy neutrophils resulted in complete inhibition of NET formation. Oxidative stress with free radical production is one of the proposed mechanisms by which LF EMF exposure affects cellular behaviour [4,13]. Neutrophils exposed to 50-Hz EMFs showed increased ROS and free radical production [13].…”

Section: Resultsmentioning

confidence: 99%

“…Patients with chronic granulomatous disease highlight the importance of functional NADPH oxidase activity, since these patients fail to maintain phagocytosis or generate NETs [12]. Even though NET formation involves activation of intracellular signalling pathways that have been linked to the effects of LF EMFs, such as ROS production and NADPH oxidase activation [13,14], a correlation between NET formation by neutrophils and LF EMF exposure has not been studied yet. In an ex vivo experimental set-up we show that LF EMF exposure is able to enhance NET formation in neutrophils for which the NADPH pathway and subsequent ROS production are essential.…”

Section: Introductionmentioning

confidence: 99%

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Low-Frequency Electromagnetic Field Exposure Enhances Extracellular Trap Formation by Human Neutrophils through the NADPH Pathway

et al. 2015

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Low-frequency (LF) electromagnetic fields (EMFs) are abundantly present in modern society, and the potential biological consequences of exposure to these fields are under intense debate. Immune cells are suggested as possible target cells, though a clear mechanism is lacking. Considering their crucial role in innate immune activation, we selected an ex vivo exposure set-up with human neutrophils to investigate a possible correlation between neutrophil extracellular trap (NET) formation and LF EMF exposure. Our study shows that formation of NETs is enhanced by LF EMF exposure. Enhanced NET formation leads to increased antimicrobial properties as well as damage to surrounding cells. We found that LF-EMF-induced NET formation is dependent on the NADPH oxidase pathway and production of reactive oxygen species. Additionally, LF EMF exposure does not influence autophagy and PAD4 activity. Our study provides a mechanism by which exposure to LF EMFs could influence the innate immune system.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Low-Frequency Electromagnetic Field Exposure Enhances Extracellular Trap Formation by Human Neutrophils through the NADPH Pathway

et al. 2015

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“…Indeed, it has previously been reported that isolated neutrophils, which appear to be highly sensitive to both static and alternating magnetic fields [39], under the exposure to strong SMF (0.6-2 T) generated significantly less superoxide anions than those detected in controls [40]. Furthermore, in a recent study a significant decline in ROS production by human peripheral blood neutrophils has been shown after 15 min exposure to SMF (approx.…”

Section: Discussionmentioning

confidence: 95%

Exposure to inhomogeneous static magnetic field beneficially affects allergic inflammation in a murine model

Csillag

Kumar

Szabó

et al. 2014

J. R. Soc. Interface.

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Previous observations suggest that static magnetic field (SMF)-exposure acts on living organisms partly through reactive oxygen species (ROS) reactions. In this study, we aimed to define the impact of SMF-exposure on ragweed pollen extract (RWPE)-induced allergic inflammation closely associated with oxidative stress. Inhomogeneous SMF was generated with an apparatus validated previously providing a peak-to-peak magnetic induction of the dominant SMF component 389 mT by 39 T m 21 lateral gradient in the in vivo and in vitro experiments, and 192 mT by 19 T m 21 in the human study at the 3 mm target distance. Effects of SMF-exposure were studied in a murine model of allergic inflammation and also in human provoked skin allergy. We found that even a single 30-min exposure of mice to SMF immediately following intranasal RWPE challenge significantly lowered the increase in the total antioxidant capacity of the airways and decreased allergic inflammation. Repeated (on 3 consecutive days) or prolonged (60 min) exposure to SMF after RWPE challenge decreased the severity of allergic responses more efficiently than a single 30-min treatment. SMF-exposure did not alter ROS production by RWPE under cell-free conditions, while diminished RWPEinduced increase in the ROS levels in A549 epithelial cells. Results of the human skin prick tests indicated that SMF-exposure had no significant direct effect on provoked mast cell degranulation. The observed beneficial effects of SMF are likely owing to the mobilization of cellular ROS-eliminating mechanisms rather than direct modulation of ROS production by pollen NAD(P)H oxidases.

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“…This has been supported by a number of studies, eg., an increase in the O 2 -in cell line immune cells or in primary immune cells (Simkó et al, 2001;Rollwitz et al, 2004;Lupke et al, 2004;Frahm et al, 2006Frahm et al, , 2010Mannerling et al, 2010;Poniedzialek et al, 2013). Other studies have shown that the ELF-MF enhanced the exogenous oxidative stress by, for example, inactivation of hexokinase and depletion of reduced glutathione in red blood cells (Fiorani et al, 1997), or increased production of reactive oxigen species in blood platelets (Henrykowska et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Effect of 50-Hz Sinusoidal Magnetic Field on the Production of Superoxide Anion and the Expression of Heat-shock Protein 70 in RAW264 Cells

Pooam¹,

Nakayama²,

Nishigaki³

et al. 2017

IJC

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There is a growing concern if the power-line frequency (50/60 Hz) magnetic field (termed in this paper ELF-MF) increases cancer risks. Since one of the major causes of cancer is cellular oxidative stress, whether the ELF-MF increases the oxidative stress is a central problem in the studies on the biological effect of the ELF-MF. Here, we have investigated the effect of 50-Hz sinusoidal magnetic field on the production of O 2 -, the expression of heat shock protein (HSP) 70 and the mitochondrial membrane potential in cell line macrophage RAW264 cells. Macrophages were exposed to or not exposed to 0.1-mT or 0.5-mT, 50-Hz sinusoidal magnetic field and were subjected to (1) assay for O 2 -(2) analysis of the expression of HSP70, and (3) measurement of the mitochondrial membrane potential with a fluorescent indicator. The 50-Hz magnetic field enhanced production of O 2 -and the expression of HSP70, both of which are consistent with previous studies. The exposure to 50-Hz magnetic field decreased mitochondrial membrane potential indicating the diminished activity of mitochondria. The uncoupler of mitochondrial function, carbonyl cyanide p-trifluoromethoxyphenylhydrazone diminished the membrane potential, as expected. On the other hand, it increased the production of O 2 -. The results collectively suggest that the 50-Hz magnetic field diminished the mitochondrial membrane potential, which led to the increase in the production of O 2 -and the expression of HSP70 protein.

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The effect of electromagnetic field on reactive oxygen species production in human neutrophilsin vitro

Cited by 29 publications

References 40 publications

Low-Frequency Electromagnetic Field Exposure Enhances Extracellular Trap Formation by Human Neutrophils through the NADPH Pathway

Low-Frequency Electromagnetic Field Exposure Enhances Extracellular Trap Formation by Human Neutrophils through the NADPH Pathway

Exposure to inhomogeneous static magnetic field beneficially affects allergic inflammation in a murine model

Effect of 50-Hz Sinusoidal Magnetic Field on the Production of Superoxide Anion and the Expression of Heat-shock Protein 70 in RAW264 Cells

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