The Effect of Elasticity of Gelatin Nanoparticles on the Interaction with Macrophages

Yıldırım, Metin; Weiss, Agnes-Valencia; Schneider, Marc

doi:10.3390/pharmaceutics15010199

Cited by 22 publications

(14 citation statements)

References 50 publications

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“…PS, stiff, and large being the characteristic levels that increased uptake of LPNPs corresponds with findings from other groups throughout literature when these characteristics were tested individually. − When all three favorable characteristics were present (PS-St-L), there was the greatest uptake by a large margin. This is the only formulation that produced significantly higher uptake compared with other PS-coated LPNPs.…”

Section: Resultssupporting

confidence: 80%

“…Nanoparticle properties can also affect macrophages’ ability to endocytose nanoparticles through different pathways. Studies modulating one property at a time (i.e., surface coating, size, or stiffness) indicate that nanoparticles with PS, − stiffer nanoparticles, − and larger nanoparticles − tend to be taken up faster and to a higher degree than those without PS, those that are softer, and those that are smaller. However, the impact of the interactions among these characteristics on macrophage uptake has not been widely studied.…”

Section: Introductionmentioning

confidence: 99%

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Modulating Lipid-Polymer Nanoparticles’ Physicochemical Properties to Alter Macrophage Uptake

Bender,

Sircar,

Taubenfeld

et al. 2024

ACS Biomater. Sci. Eng.

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Macrophage uptake of nanoparticles is highly dependent on the physicochemical characteristics of those nanoparticles. Here, we have created a collection of lipid-polymer nanoparticles (LPNPs) varying in size, stiffness, and lipid makeup to determine the effects of these factors on uptake in murine bone marrow-derived macrophages. The LPNPs varied in diameter from 232 to 812 nm, in storage modulus from 21.2 to 287 kPa, and in phosphatidylserine content from 0 to 20%. Stiff, large nanoparticles with a coating containing phosphatidylserine were taken up by macrophages to a much higher degree than any other formulation (between 9.3× and 166× higher than other LPNPs). LPNPs with phosphatidylserine were taken up most by M2-polarized macrophages, while those without were taken up most by M1-polarized macrophages. Differences in total LPNP uptake were not dependent on endocytosis pathway(s) other than phagocytosis. This work acts as a basis for understanding how the interactions between nanoparticle physicochemical characteristics may act synergistically to facilitate particle uptake.

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Section: Resultssupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Modulating Lipid-Polymer Nanoparticles’ Physicochemical Properties to Alter Macrophage Uptake

Bender,

Sircar,

Taubenfeld

et al. 2024

ACS Biomater. Sci. Eng.

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show abstract

“…The landmark amino acids in gelatin highlighted the enormous potential to functionalize the gelatin-based materials with ligands in malignancy targeting. 169 Gelatin has a variety of functional groups, enabling easy surface modification for drug treatment to target tumors, tissues and organs. The concept of immunoliposomes, which involve the conjugation of antibodies to liposomes, was initially introduced in the early 1980s.…”

Section: Applications Of Gelatin Of Micro/nanostructures In Therapeut...mentioning

confidence: 99%

Chemical and Structural Engineering of Gelatin-Based Delivery Systems for Therapeutic Applications: A Review

Jia,

Fan,

Chen

et al. 2024

Biomacromolecules

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As a biodegradable and biocompatible protein derived from collagen, gelatin has been extensively exploited as a fundamental component of biological scaffolds and drug delivery systems for precise medicine. The easily engineered gelatin holds great promise in formulating various delivery systems to protect and enhance the efficacy of drugs for improving the safety and effectiveness of numerous pharmaceuticals. The remarkable biocompatibility and adjustable mechanical properties of gelatin permit the construction of active 3D scaffolds to accelerate the regeneration of injured tissues and organs. In this Review, we delve into diverse strategies for fabricating and functionalizing gelatin-based structures, which are applicable to gene and drug delivery as well as tissue engineering. We emphasized the advantages of various gelatin derivatives, including methacryloyl gelatin, polyethylene glycolmodified gelatin, thiolated gelatin, and alendronate-modified gelatin. These derivatives exhibit excellent physicochemical and biological properties, allowing the fabrication of tailor-made structures for biomedical applications. Additionally, we explored the latest developments in the modulation of their physicochemical properties by combining additive materials and manufacturing platforms, outlining the design of multifunctional gelatin-based micro-, nano-, and macrostructures. While discussing the current limitations, we also addressed the challenges that need to be overcome for clinical translation, including high manufacturing costs, limited application scenarios, and potential immunogenicity. This Review provides insight into how the structural and chemical engineering of gelatin can be leveraged to pave the way for significant advancements in biomedical applications and the improvement of patient outcomes.

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“…The molecular chain of gelatin has a collagen-like amino acid composition on its surface and contains a high number of hydrophilic groups, which can easily form hydrogen bonds. Thus, gelatin is highly water soluble and can easily bind to drug molecules ( Yildirim et al, 2023 ). Ramanathan et al, used polyhydroxy butyric acid/gelatin nanofibers to release 5-fluorouracil (5-FU) (highly hydrophilic) and methotrexate (MT) (less hydrophilic).…”

Section: Properties Of Gelatin For An Anticancer Ddnsmentioning

confidence: 99%

Gelatin-based anticancer drug delivery nanosystems: A mini review

Jiang

Zhang

et al. 2023

Front. Bioeng. Biotechnol.

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Drug delivery nanosystems (DDnS) is widely developed recently. Gelatin is a high-potential biomaterial originated from natural resources for anticancer DDnS, which can effectively improve the utilization of anticancer drugs and reduce side effects. The hydrophilic, amphoteric behavior and sol-gel transition of gelatin can be used to fulfill various requirements of anticancer DDnS. Additionally, the high number of multifunctional groups on the surface of gelatin provides the possibility of crosslinking and further modifications. In this review, we focus on the properties of gelatin and briefly elaborate the correlation between the properties and anticancer DDnS. Furthermore, we discuss the applications of gelatin-based DDnS in various cancer treatments. Overall, we have summarized the excellent properties of gelatin and correlated with DDnS to provide a manual for the design of gelatin-based materials for DDnS.

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The Effect of Elasticity of Gelatin Nanoparticles on the Interaction with Macrophages

Cited by 22 publications

References 50 publications

Modulating Lipid-Polymer Nanoparticles’ Physicochemical Properties to Alter Macrophage Uptake

Modulating Lipid-Polymer Nanoparticles’ Physicochemical Properties to Alter Macrophage Uptake

Chemical and Structural Engineering of Gelatin-Based Delivery Systems for Therapeutic Applications: A Review

Gelatin-based anticancer drug delivery nanosystems: A mini review

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