The effect of eight weeks of combined training on Brain-Derived Neurotrophic Factor- MIR132 levels and Cognitive Function in Streptozotocin-induced Diabetic Rats

Abstract

Introduction: Neurotrophins are factors that protect nerve tissue and improve the function of the central nervous system. In diabetes, despite insulin resistance, Brain-derived neurotrophic factor (BDNF) levels and function are impaired, while physical activity has a great impact on brain function and BDNF levels. The purpose of this study was to evaluate the effect of eight weeks of combined training (resistance circular-aerobic) on blood sugar, insulin, MIR132, and BDNF levels, in streptozotocin-induced diabetic rats. Methods: 40 Spraguedali rats were randomly divided into four groups: h.s (healthy sports), h.n.s(healthy non-sports), d.s (diabetes sports) d.n.s (diabetes non-sport). Rats in the training groups performed combined training for 8 weeks and the subjects' memory was assessed using a cognitive function test in a water maze. BDNF levels in the hippocampus were measured by western blotting and to measure MIR132 by the real-time PCR Results: The present study showed that eight weeks of combined exercise resulted in a significant reduction in fasting blood sugar in the h.s group and BDNF levels of the cerebral hippocampus were also observed in the d.s group compared to the d.n.s group. But No significant difference was observed in hippocampal mir-132 between groups. By examining serum mir-132, a significant difference was observed between the d.n.s with the d.s and h.s, which was associated with a significant decrease in this variable in the exercise (h.s and d.s) groups compared to the inactive patient. Conclusions: 8 weeks of combined training reduces BDNF in the hippocampus of the brain and the level of cognitive performance in the: h.s and ds groups compared to h.n.s and d.n.s groups increased, but no significant difference was observed between the test groups in the miR-132 variable of the hippocampus, so this type of training is very effective in improving the complications such as complications of retinopathy and neuropathy of type 2 diabetes.