The effect of earthworm extract on mice S180 tumor growth and apoptosis

Deng, Zhenhan; Gao, Shanshan; Xiao, Xiang; Yin, Ni; Ma, Shiyang; Li, Yusheng

doi:10.1016/j.biopha.2019.108979

Cited by 12 publications

(10 citation statements)

References 39 publications

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“…The procedure of EE preparation was the same as our previous study (18,19). First of all, earthworm (Diehei II) and PBS (0.02 m, pH ≤7.2) homogenates were centrifuged (5,000 ×g, 4 ℃, 10 min).…”

Section: Preparation Of Eementioning

confidence: 99%

The therapeutic effects of earthworm extract on deep second-degree burn wound healing

He¹,

Xie²,

Cheng³

et al. 2021

Ann Palliat Med

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Background: To investigated the effect of earthworm extract (EE) on deep second-degree burn wound healing process.Methods: A burn wound model was created on the mice's skin and was subject to different treatments: the control group received no treatment; the Jingwanhong (JWH: a well-established, widely used external ointment for treating burn wounds) group was treated with 0.1 g of JWH cream and spray it on the wound surface; the EE group was treated with 0.1 mL of EE solution. All the mice were sacrificed at 3, 7, 11, and 15 days after injury (n=6/group/time point). Macroscopic observation, wound healing rate (WHR), wound healing time (WHT), water content (WC), hydroxyproline (Hyp) content, histological, and hematological analyses were performed at the burn wound sites.Results: Better, faster burn wound healing in the JWH and EE groups than the control group at 15 days after injury were detected at the wound sites. Compared to the control group, the EE group had higher WHR, shorter WHT, lower WC, higher Hyp content, more fibroblasts, fibrocytes, and capillary endothelial cells; in addition, they showed greater capillary endothelial cell grouping at the wound sites during the healing process. This group also showed more platelets, white blood cells (WBCs), and neutrophilic granulocytes in serum at the early stages after burn injury.Conclusions: EE could effectively promote skin wound healing by decreasing edema, suppressing fibrosis, activating angiogenesis and epithelial regeneration, inhibiting scar formation, and reducing the risk of infection. Thus, it could be made into a promising healing agent for burn wound.

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Section: Preparation Of Eementioning

confidence: 99%

The therapeutic effects of earthworm extract on deep second-degree burn wound healing

He¹,

Xie²,

Cheng³

et al. 2021

Ann Palliat Med

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“…Further biochemical studies need to be followed up to investigate the mechanistic principles involved in RUNX3mediated inhibition of EMT in colitis associated colon cancer. In the development of EMT, cancer cells lose the properties of epithelial cells and develop mesenchymal cell properties, including overexpression of vimentin [12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

“…It is currently clear that EMT has implications on cancer metastasis by triggering the loss of cell-cell adhesion to facilitate the invasion of cancer cell. However, the mechanism of RUNX3-mediated suppression of cancer metastasis remains unclear and the role of RUNX3 in colorectal cancer has not yet been well studied [ 11 – 14 ]. Further biochemical studies need to be followed up to investigate the mechanistic principles involved in RUNX3-mediated inhibition of EMT in colitis associated colon cancer.…”

Section: Introductionmentioning

confidence: 99%

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Coelomic Fluid of Eisenia fetida Ameliorates Cetuximab to Reduce K‐Ras and Vimentin Expression through Promoting RUNX3 in an AOM/DSS‐Induced Colitis Associated Colon Cancer

Permana

Fityanti

Norahmawati

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Ulcerative colitis is a major risk factor that increases the occurrence of colorectal cancer. In colorectal cancer due to colitis, intestinal inflammation plays an important role which causes DNA damage. The aim of this study is to investigate the anticancer effect of coelomic fluid of Eisenia fetida (CFEF) and cetuximab combinations. Colitis associated colon cancer was induced in BALB/c mice by DSS/AOM. The mice were randomly divided into six groups: group 1 received vehicle (control), groups 2–6 received DSS/AOM, groups 3–5 received cetuximab + CFEF (30, 60, or 120 mg/kgBW), and group 6 received CFEF only. After the 12th week of treatments, the colon tissues were removed for histological examination and immune-fluorescence. Intestinal Epithelial Cells (CECs) were analyzed by flow cytometer. Administration of CFEF significantly decreased the severity of DSS/AOM-induced CAC in a dose-dependent manner. The combinations of CFEF-cetuximab were revealed by histological change. The CFEF significantly reduced the severity scores (P<0.05). The combinations of CFEF-cetuximab significantly inhibited K-Ras and vimentin expressions, whereas the percentage of RUNX3 significantly increased in CECs. The increasing of RUNX3 could prevent EMT, so that it can decrease K-Ras and vimentin to suppressed cell invasion and migration by CFEF. Our results suggest that CFEF has the therapeutic potential to CAC.

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“…Pheretima was documented as a TCM in the earliest known treatise of Chinese material medica, “Shennong Bencao Jing”, written 2000 years ago [9], and was widely used for the treatment of fever, asthma and stroke [1]. Increasing evidences indicate that Pheretima has wide therapeutic properties, including anti-inflammatory activity [10], anti-oxidative activity [11], fibrinolytic activity [12], anti-asthmatic activity [13], as well as promoting tumor apoptosis [14] and bone regeneration [15]. According to ChP 2015, there are four Pheretima animals, namely Amynthas aspergillus E. Perrier, Amynthas pectiniferus Michaelsen, Metaphire vulgaris Chen and Metaphire guillelmi Michaelsen.…”

Section: Introductionmentioning

confidence: 99%

Specific DNA identification of Pheretima in the Naoxintong capsule

Zhu

Shaw

et al. 2019

Chin Med

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Background Pheretima is a minister drug in Naoxintong capsule (NXTC), a well-known traditional Chinese medicine (TCM) formula for the treatment of cardiovascular and cerebrovascular diseases. Owing to the loss of morphological and microscopic characteristics and the lack of recognized chemical marker, it is difficult to identify Pheretima in NXTC. This study aims to evaluate the feasibility of using DNA techniques to authenticate Pheretima, especially when it is processed into NXTC. Methods DNA was extracted from crude drugs of the genuine and adulterant species, as well as nine batches of NXTCs. Based on mitochondrial cytochrome c oxidase subunit I (COI) gene, specific primers were designed for two genera of genuine species, Metaphire and Amynthas, respectively. PCR amplification was performed with the designed primers on crude drugs of Pheretima and NXTCs. The purified PCR products were sequenced and the obtained sequences were identified to species level with top hit of similarity with BLAST against GenBank nucleotide database. Results Primers MF2R2 and AF3R1 could amplify specific DNA fragments with sizes around 230–250 bp, both in crude drugs and NXTC. With sequencing and the BLAST search, identities of the tested samples were found. Conclusion This study indicated that the molecular approach is effective for identifying Pheretima in NXTC. Therefore, DNA identification may contribute to the quality control and assurance of NXTC.

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The effect of earthworm extract on mice S180 tumor growth and apoptosis

Cited by 12 publications

References 39 publications

The therapeutic effects of earthworm extract on deep second-degree burn wound healing

The therapeutic effects of earthworm extract on deep second-degree burn wound healing

Coelomic Fluid of Eisenia fetida Ameliorates Cetuximab to Reduce K‐Ras and Vimentin Expression through Promoting RUNX3 in an AOM/DSS‐Induced Colitis Associated Colon Cancer

Specific DNA identification of Pheretima in the Naoxintong capsule

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