The effect of dual frequency cyclic compression on matrix deposition by osteoblast-like cells grown in 3D scaffolds and on modulation of VEGF variant expression

Dumas, Virginie; Perrier, Anthony; Malaval, Luc; Laroche, Norbert; Guignandon, Alain; Vico, Laurence; Rattner, Aline

doi:10.1016/j.biomaterials.2009.02.048

Cited by 48 publications

(47 citation statements)

References 40 publications

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“…Our original culture chamber was developed to be compatible with the Zetos ® compression device (David et al, 2008;Davies et al, 2006;Dumas et al, 2009) which G Bouet et al In vitro 3D bone culture model can exert cyclic compression on a mobile piston through a piezo-electric actuator controlled by a computer program (Fig. 1D).…”

Section: Mechanical Loadingmentioning

confidence: 99%

“…A complete description of the device is given in Jones et al (Jones et al, 2003). In our experiments, the scaffolds were subject to a composite mechanical signal pattern previously used by Dumas et al (Dumas et al, 2009). This signal is a sinusoidal waveform at 3 Hz frequency with an amplitude of 4 µm, to which are superimposed 25 Hz vibrations of < 1.5 µm amplitude (Fig.…”

Section: Mechanical Loadingmentioning

confidence: 99%

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Validation of an in vitro 3D bone culture model with perfused and mechanically stressed ceramic scaffold

Bouet¹,

Cruel²,

Laurent³

et al. 2015

eCM

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An engineered three dimensional (3D) in vitro cell culture system was designed with the goal of inducing and controlling in vitro osteogenesis in a reproducible manner under conditions more similar to the in vivo bone microenvironment than traditional two-dimensional (2D) models. This bioreactor allows efficient mechanical loading and perfusion of an original cubic calcium phosphate bioceramic of highly controlled composition and structure. This bioceramic comprises an internal portion containing homogeneously interconnected macropores surrounded by a dense layer, which minimises fluid flow bypass around the scaffold. This dense and flat layer permits the application of a homogeneous loading on the bioceramic while also enhancing its mechanical strength. Numerical modelling of constraints shows that the system provides direct mechanical stimulation of cells within the scaffold. Experimental results establish that under perfusion at a steady flow of 2 µL/min, corresponding to 3 ≤ Medium velocity ≤ 23 µm/s, mouse calvarial cells grow and differentiate as osteoblasts in a reproducible manner, and lay down a mineralised matrix. Moreover, cells respond to mechanical loading by increasing C-fos expression, which demonstrates the effective mechanical stimulation of the culture within the scaffold. In summary, we provide a "proof-of-concept" for osteoblastic cell culture in a controlled 3D culture system under perfusion and mechanical loading. This model will be a tool to analyse bone cell functions in vivo, and will provide a bench testing system for the clinical assessment of bioactive bone-targeting molecules under load.

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Section: Mechanical Loadingmentioning

confidence: 99%

Section: Mechanical Loadingmentioning

confidence: 99%

Validation of an in vitro 3D bone culture model with perfused and mechanically stressed ceramic scaffold

Bouet¹,

Cruel²,

Laurent³

et al. 2015

eCM

View full text Add to dashboard Cite

show abstract

“…For compressive loading, as well as transmission of forces through the scaffold itself, fluid may also move within the scaffold pores because of the applied loading. For bone constructs, materials including hydroxyapatite (HA) 48 or tricalcium phosphate (TCP) scaffolds, 49 polyurethane (PU) sponges, 50 collagen-based scaffolds 51 and electrospun matrices have been commonly used.…”

Section: Choice Of Scaffolding Materialsmentioning

confidence: 99%

“…These fibrous matrices support progenitor and mature bone cell growth and are suitable for dynamic tensile stimulation of cells or perfusion flow cultures. 53 Specific materials: HA-macroporous HA scaffolds with 500 or 300 mm pore size, 4 mm high and 10 mm diameter (BIOCETIS, Boulogne-sur-Mer, France); 48 Seeding scaffolds Cell suspensions are generally introduced into scaffolds in small volumes of media (100-300 ml) added dropwise and left for a few hours to allow cells to attach 50,54 before more media are then added to cover the scaffolds and to ensure cell survival. Dental wire may be used to prevent scaffolds from floating when additional media are added, 50 after which scaffolds are often left overnight 49,54 or for several days 50 before stimulation is applied (Figure 5a).…”

Section: Loading Postmentioning

confidence: 99%

“…The use of a load cell allows the monitoring and adjustment of the applied forces, even if the mechanical properties change during the culture period because of extracellular matrix deposition. 48,50 In direct compression, the material may also be confined or unconfined, 50 the latter allowing the flow of fluid in and out of the construct during loading. This flow of fluid may act like the flow of interstitial fluid within canaliculi during loading of bones in vivo.…”

Section: D Compressionmentioning

confidence: 99%

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Preclinical models for in vitro mechanical loading of bone-derived cells

Delaine-Smith¹,

Javaheri²,

Edwards³

et al. 2015

BoneKEy Reports

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It is well established that bone responds to mechanical stimuli whereby physical forces are translated into chemical signals between cells, via mechanotransduction. It is difficult however to study the precise cellular and molecular responses using in vivo systems. In vitro loading models, which aim to replicate forces found within the bone microenvironment, make the underlying processes of mechanotransduction accessible to the researcher. Direct measurements in vivo and predictive modeling have been used to define these forces in normal physiological and pathological states. The types of mechanical stimuli present in the bone include vibration, fluid shear, substrate deformation and compressive loading, which can all be applied in vitro to monolayer and three-dimensional (3D) cultures. In monolayer, vibration can be readily applied to cultures via a low-magnitude, high-frequency loading rig. Fluid shear can be applied to cultures in multiwell plates via a simple rocking platform to engender gravitational fluid movement or via a pump to cells attached to a slide within a parallel-plate flow chamber, which may be micropatterned for use with osteocytes. Substrate strain can be applied via the vacuum-driven FlexCell system or via a four-point loading jig. 3D cultures better replicate the bone microenvironment and can also be subjected to the same forms of mechanical stimuli as monolayer, including vibration, fluid shear via perfusion flow, strain or compression. 3D cocultures that more closely replicate the bone microenvironment can be used to study the collective response of several cell types to loading. This technical review summarizes the methods for applying mechanical stimuli to bone cells in vitro.

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Assessing the potential of mesenchymal stem cells in craniofacial bone repair and regeneration

Waddington

Jones

Moseley

2016

Tissue Engineering and Regeneration in Dentistry

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The effect of dual frequency cyclic compression on matrix deposition by osteoblast-like cells grown in 3D scaffolds and on modulation of VEGF variant expression

Cited by 48 publications

References 40 publications

Validation of an in vitro 3D bone culture model with perfused and mechanically stressed ceramic scaffold

Validation of an in vitro 3D bone culture model with perfused and mechanically stressed ceramic scaffold

Preclinical models for in vitro mechanical loading of bone-derived cells

Assessing the potential of mesenchymal stem cells in craniofacial bone repair and regeneration

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