The effect of DNA priming-protein boosting on enhancing humoral immunity and protecting mice against lethal HSV infections

Soleimanjahi, Hoorieh; Roostaee, Mohammad Hassan; Rasaee, Mohammad Javad; Mahboudi, F; Kazemnejad, Anoshirvan; Bamdad, Taravat; Zandi, Keivan

doi:10.1111/j.1574-695x.2005.00015.x

Cited by 15 publications

(8 citation statements)

References 26 publications

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“…HSV‐2 neutralizing antibody titration was performed as described previously, with some modifications (Soleimanjahi et al , 2006). Briefly, sera were inactivated at 56 °C for 30 min before serial dilution in DMEM.…”

Section: Methodsmentioning

confidence: 99%

Enhancement of protective humoral immune responses against Herpes simplex virus-2 in DNA-immunized guinea-pigs using protein boosting

Fotouhi

Soleimanjahi

Roostaee

et al. 2008

FEMS Immunology &Amp; Medical Microbiology

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Genital Herpes is a common sexually transmitted disease that is caused mostly by Herpes simplex virus type 2 (HSV-2). Its prevalence has increased in developing countries in spite of the availability of valuable antiviral drug therapy. Considering the importance of HSV-2 infections, effective vaccines remain the most likely hope for controlling the spread of HSV diseases. In the present study, the complete HSV-2 glycoprotein D gene was isolated and cloned into different plasmid vectors to construct a DNA vaccine and prepare recombinant subunit vaccines using a baculovirus expression system. The vaccines were tested alone or in combination to evaluate their ability to induce protective immunity in guinea-pigs against genital HSV infections. Immunization elicited humoral responses as measured by neutralization tests and enzyme-linked immunosorbent assay, and immunized animals had less severe genital skin disease as well as reduced replication of the challenging virus in the genital tract during experimental infection. Our results further demonstrate that DNA priming-protein boosting induced a neutralizing antibody titer higher than that obtained with DNA-DNA vaccination. The massive increase of antibody titer following DNA priming-protein boosting might be attributed to a recall of B cell memory.

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Section: Methodsmentioning

confidence: 99%

Enhancement of protective humoral immune responses against Herpes simplex virus-2 in DNA-immunized guinea-pigs using protein boosting

Fotouhi

Soleimanjahi

Roostaee

et al. 2008

FEMS Immunology &Amp; Medical Microbiology

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“…cell mediated cytotoxicity (ADCC), and virusspecific cellular responses mediated by both CD4+ and CD8+ T lymphocytes (15). In this study, we tried to run Western blotting using HSV1 whole particles and baculo virus derived HSV-gD1 as antigens and compared the results with virus neutralization test (VNT).…”

Section: Detection Of Herpes Simplex Virus Antibodies …mentioning

confidence: 99%

“…Herpes simplex virus (HSV) was isolated from labial vesicles of a patient and identified as HSV1 using monoclonal antibody (15). HeLa cells were infected at a multiplicity of infection (moi) of 1 pfu/cell, after one hour adsorption, unabsorbed virus suspension was discarded and the cells were washed two times by sterile phosphate buffer saline (pH=7).…”

Section: Virus Propagationmentioning

confidence: 99%

Detection of Herpes Simplex Virus Antibodies Using the Whole Virus and Recombinant gD

Meshkat

Soleimanjahi

et al. 2009

Iran J Virol

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Background and Aims: Herpes simplex virus type 1 (HSV1) remains a potentially serious health problem world wide. All infected people, including asymptomatic ones, are potential sources for virus transmission. Virus envelope contains at least 13 glycoproteins, which glycoprotein D is the major target of immune responses. The aim of this study was development of a specific method that is a more rapid, sensitive and specific test compared to the virus neutralization test which is applied as gold standard test. Methods: In this study, the Western blot technique using crude HSV1 whole particle and baculovirus derived glycoprotein D of HSV1 as antigens was set. Human sera were analyzed by virus neutralization test and then serum samples with reciprocal virus neutralization antibody titers of 32, 64, and 128 were taken to be analyzed by Western blotting. Results: It was shown that there was a very good correlation between results obtained from virus neutralization antibody titers and those of Western blotting. Western blotting using recombinant glycoprotein D of HSV1 as an antigen showed positive results similar to the whole HSV1 antigen. Conclusion: Our study showed the Western blotting using recombinant glycoprotein D can replace virus neutralization test in diagnosis of HSV1 infections.

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“…A wild-type HSV-1 strain was isolated from a cold sore lesion of a patient. The virus was confirmed as HSV-1 with an immunofluorescence assay using a HSV-1-specific monoclonal antibody [11]. The neurovirulence of the virus was proven by injecting the virus into mice and then isolating the virus from brains of dead mice.…”

Section: Methodsmentioning

confidence: 99%

Withdrawal from Morphine Reduces Cell-Mediated Immunity against Herpes Simplex Virus Generated by Natural Immunization

Jamali

Soleimanjahi²,

Moin³

et al. 2012

Neuroimmunomodulation

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In a previous study, the authors have shown that herpes simplex virus type 1 (HSV-1) glycoprotein B DNA vaccine but not live vaccine (non-virulent KOS strain) failed to induce protective immunity against acute HSV-1 challenge in morphine-dependent mice. The present study reports the effect of morphine withdrawal on protective immunity induced by live HSV-1 immunization. BALB/c mice were vaccinated with KOS strain as a live vaccine. Three weeks later, they were exposed to morphine for 14 days. On day 14, withdrawal was induced by administration of normal saline instead of morphine. One day later, immune responses against HSV-1 were assessed by measuring cytotoxicity, lymphocyte proliferation and interferon-γ production. Protection against HSV-1 was assessed by measuring the mortality rate after acute HSV-1 challenge. The results showed that withdrawal from morphine reduces protective immunity against acute HSV-1 challenge. These findings raise the possibility that withdrawal from morphine may increase the susceptibility of drug addicts to infectious diseases.

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The effect of DNA priming-protein boosting on enhancing humoral immunity and protecting mice against lethal HSV infections

Cited by 15 publications

References 26 publications

Enhancement of protective humoral immune responses against Herpes simplex virus-2 in DNA-immunized guinea-pigs using protein boosting

Enhancement of protective humoral immune responses against Herpes simplex virus-2 in DNA-immunized guinea-pigs using protein boosting

Detection of Herpes Simplex Virus Antibodies Using the Whole Virus and Recombinant gD

Withdrawal from Morphine Reduces Cell-Mediated Immunity against Herpes Simplex Virus Generated by Natural Immunization

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