The effect of DMSA-functionalized magnetic nanoparticles on transendothelial migration of monocytes in the murine lung via a β2 integrin-dependent pathway

Valois, Caroline R.A.; Braz, Juliana M.; Nunes, Eloiza S; Vinolo, Marco Aurélio Ramirez; Lima, E.C.D.; Curi, Rui; Kuebler, Wolfgang M.; Azevedo, Ricardo Bentes

doi:10.1016/j.biomaterials.2009.09.053

Cited by 73 publications

(36 citation statements)

References 47 publications

(72 reference statements)

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“…Results of the present investigation indicated that while using DMSA-Fe 3 O 4 with an average hydrodynamic size of 68.4±11.39 nm, a majority of MNPs were taken up by RPMI-8226 cells through membrane-bound vesicles and shuttled to the cytosol ( Figure 2C and D). Furthermore, results indicated that there was negative charge on the surface of DMSA-MNPs or BTZ and GA solution, 26,36 hence, we deduced that BTZ and GA may have been polymerized on the surface of DMSA-MNPs nanoparticles for nanocomposites through hydrogen bond combination and not static electricity adsorption. Thus, the ability to suppress efflux pumps in the cell membrane and transport drugs into the cell might be one of the proposed mechanisms allowing MNPs to improve treatment efficiency.…”

mentioning

confidence: 89%

Inducing cell cycle arrest and apoptosis by dimercaptosuccinic acid modified Fe3O4 magnetic nanoparticles combined with nontoxic concentration of bortezomib and gambogic acid in RPMI-8226 cells

Zhang¹,

Qiao²,

Wang³

et al. 2015

IJN

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The purpose of this study was to determine the potential benefits of combination therapy using dimercaptosuccinic acid modified iron oxide (DMSA-Fe 3 O 4 ) magnetic nanoparticles (MNPs) combined with nontoxic concentration of bortezomib (BTZ) and gambogic acid (GA) on multiple myeloma (MM) RPMI-8226 cells and possible underlying mechanisms. The effects of BTZ-GA-loaded MNP-Fe 3 O 4 (BTZ-GA/MNPs) on cell proliferation were assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,4,-diphenyltetrazolium bromide (MTT) method. Cell cycle and apoptosis were detected using the terminal deoxyribonucleotidyl transferase (TdT)-mediated biotin-16-dUTP nick-end labeling (TUNEL) assay and flow cytometry (FCM). Furthermore, DMSA-Fe 3 O 4 MNPs were characterized in terms of distribution, apoptotic morphology, and cellular uptake by transmission electron microscopy (TEM) and 4,6-diamidino-2-phenylindole (DAPI) staining. Subsequently, the effect of BTZ-GA/MNPs combination on PI3K/Akt activation and apoptotic-related protein were appraised by Western blotting. MTT assay and hematoxylin and eosin (HE) staining were applied to elevate the functions of BTZ-GA/MNPs combination on the tumor xenograft model and tumor necrosis. The results of this study revealed that the majority of MNPs were quasi-spherical and the MNPs taken up by cells were located in the endosome vesicles of cytoplasm. Nontoxic concentration of BTZ-GA/MNPs increased G 2 /M phase cell cycle arrest and induced apoptosis in RPMI-8226 cells. Furthermore, the combination of BTZ-GA/MNPs activated phosphorylated Akt levels, Caspase-3, and Bax expression, and down-regulated the PI3K and Bcl-2 levels significantly. Meanwhile, the in vivo tumor xenograft model indicated that the treatment of BTZ-GA/MNPs decreased the tumor growth and volume and induced cell apoptosis and necrosis. These findings suggest that chemotherapeutic agents polymerized MNPs-Fe 3 O 4 with GA could serve as a better alternative for targeted therapeutic approaches to treat multiple myeloma.

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confidence: 89%

Inducing cell cycle arrest and apoptosis by dimercaptosuccinic acid modified Fe3O4 magnetic nanoparticles combined with nontoxic concentration of bortezomib and gambogic acid in RPMI-8226 cells

Zhang¹,

Qiao²,

Wang³

et al. 2015

IJN

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“…Magnetite nanoparticles were synthesized by mixing ferric and ferrous chloride aqueous solutions (2:1 molar ratio) with concentrated ammonia aqueous solution followed by vigorous stirring as described in our previous works [21][22][23]. The black magnetic precipitate was isolated by magnetic separation, washed with water and oxidized by refluxing in HCl solution under oxygen flux at 96°C, yielding a brownish colloidal suspension, indicating the efficient oxidation of magnetite to maghemite.…”

Section: Magnetic Nanoparticle Preparation and Functionalizationmentioning

confidence: 99%

High Efficacy in Hyperthermia-associated with Polyphosphate Magnetic Nanoparticles for Oral Cancer Treatment

Taboga¹

2014

J Nanomed Nanotechnol

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Nanotherapy applied to cancer treatment is constantly evolving, and new approaches to current techniques, such as magnetohyperthermia, are being implemented to solve and minimize the limitations of conventional therapeutic strategies. The purpose of this study was to investigate the action of polyphosphate-coated maghemite nanoparticles (MNPs) on oral squamous cell carcinoma. Human oral cancer cells (UM-SCC14A) were incubated with MNPs at various concentrations and subjected to cell proliferation tests (MTT), apoptosis assays and transmission electron image analysis. Viability and apoptotic events were time and dose dependent. These in vitro tests showed that at the intermediate concentration tested there is no significant toxicity, as confirmed by transmission electron microscopy. For this reason this MNPs concentration was chosen for the subsequent in vivo tests. Oral tumor induction was performed by applying the carcinogen DMBA to Syrian hamsters. Animals were then treated by magnetohyperthermia using MNPs. No signs of general clinical symptoms of toxicity or abnormal behavioral reactions were observed. However, animals treated with MNPs and exposed to the alternating magnetic field in the hyperthermia procedure exhibited a significant and time dependent cancer regression, as confirmed by histopathological analyses and immunohistochemistry. Actually, in quantitative terms of the magnetotherapy efficacy involving these polyphosphate-coated MNPs, 100% recovery (12/12) was observed in the oral cancer tumor bearing Syrian hamsters seven days after the treatment with the magnetohyperthermia procedure. Data supports the suggestion that the MNPs-mediated hyperthermia represents a promising strategy for the treatment of oral cancer.in patients with malignancies in the mouth region. Unlike many other oncology subspecialties, however, the rates of recurrence and overall survival have improved only modestly over the past decades, despite the continuous search for new therapeutic techniques [6,7]. Thus, it is expected that the identification and use of specific agents will lead to individualized treatments, which in turn will lead to significantly better results as manifested by more cures and better quality of life by decreasing toxicity [8].Over the last years, maghemite nanoparticles (MNPs) have appeared as a well-established technology and an important field of research in superparamagnetism of colloidal materials, characteristic which allows them to be guided with an external magnetic field [9]. Recently, MNPs have been investigated for therapeutic purposes such as hyperthermic treatment [10,11]. Magnetohyperthermia (MHT) represents a novel and promising therapy for cancer treatment [11,12], being capable of promoting the specific lysis of tumor cells, thereby improving patient outcomes while minimizing the subsequent toxicity effects [13]. The Citation: Candido NM, Calmon MF, Taboga SR, Bonilha JL, dos Santos MC, et al. (2014) High Efficacy in Hyperthermia-associated with Polyphosphate

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“…The sediment, containing the MNPs, was dispersed in NaCl solution and then coated with meso-2,3-dimercaptosuccinic acid (DMSA), according to the procedure previously reported for the production of stable magnetic fluid samples. 21 The total iron concentration in the MF sample as determined by atomic absorption was 17 mg/mL and the crystalline mean diameter estimated from the X-ray diffractogram (X-ray diffractometer, Shimadzu XRD 6000; Shimadzu Corporation, Kyoto, Japan) using Scherrer's equation was 8.3 nm. From these data, the concentration of nanoparticles was estimated within the as-prepared MF sample (1.5 × 10 19 nanoparticles/mL).…”

Section: Preparation and Characterization Of The Magnetic Fluid (Mf) mentioning

confidence: 99%

Anti-CEA loaded maghemite nanoparticles as a theragnostic device for colorectal cancer

Paz¹,

Santos²,

Santos³

et al. 2012

IJN

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Nanosized maghemite particles were synthesized, precoated (with dimercaptosuccinic acid) and surface-functionalized with anticarcinoembryonic antigen (anti-CEA) and successfully used to target cell lines expressing the CEA, characteristic of colorectal cancer (CRC) cells. The as-developed nanosized material device, consisting of surface decorated maghemite nanoparticles suspended as a biocompatible magnetic fluid (MF) sample, labeled MF-anti-CEA, was characterized and tested against two cell lines: a high-CEA expressing cell line (LS174T) and a low-CEA expressing cell line (HCT116). Whereas X-ray diffraction was used to assess the average core size of the as-synthesized maghemite particles (average 8.3 nm in diameter), dynamic light scattering and electrophoretic mobility measurements were used to obtain the average hydrodynamic diameter (550 nm) and the zeta-potential (-38 mV) of the as-prepared and maghemite-based nanosized device, respectively. Additionally, surface-enhanced Raman spectroscopy (SERS) was used to track the surface decoration of the nanosized maghemite particles from the very first precoating up to the attachment of the anti-CEA moiety. The Raman peak at 1655 cm -1 , absent in the free anti-CEA spectrum, is the signature of the anti-CEA binding onto the precoated magnetic nanoparticles. Whereas MTT assay was used to confirm the low cell toxicity of the MF-anti-CEA device, ELISA and Prussian blue iron staining tests performed with both cell lines (LS174T and HCT116) confirm that the as-prepared MF-anti-CEA is highly specific for CEA-expressing cells. Finally, transmission electron microscopy analyses show that the association with anti-CEA seems to increase the number of LS174T cells with internalized maghemite nanoparticles, whereas no such increase seems to occur in the HCT116 cell line. In conclusion, the MF-anti-CEA sample is a biocompatible device that can specifically target CEA, suggesting its potential use as a theragnostic tool for CEA-expressing tumors, micrometastasis, and cancer-circulating cells.

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The effect of DMSA-functionalized magnetic nanoparticles on transendothelial migration of monocytes in the murine lung via a β2 integrin-dependent pathway

Cited by 73 publications

References 47 publications

Inducing cell cycle arrest and apoptosis by dimercaptosuccinic acid modified Fe3O4 magnetic nanoparticles combined with nontoxic concentration of bortezomib and gambogic acid in RPMI-8226 cells

Inducing cell cycle arrest and apoptosis by dimercaptosuccinic acid modified Fe3O4 magnetic nanoparticles combined with nontoxic concentration of bortezomib and gambogic acid in RPMI-8226 cells

High Efficacy in Hyperthermia-associated with Polyphosphate Magnetic Nanoparticles for Oral Cancer Treatment

Anti-CEA loaded maghemite nanoparticles as a theragnostic device for colorectal cancer

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The effect of DMSA-functionalized magnetic nanoparticles on transendothelial migration of monocytes in the murine lung via a β2 integrin-dependent pathway

Cited by 73 publications

References 47 publications

Inducing cell cycle arrest and apoptosis by&nbsp;dimercaptosuccinic acid modified Fe3O4 magnetic nanoparticles combined with nontoxic concentration of bortezomib and gambogic acid&nbsp;in RPMI-8226 cells

Inducing cell cycle arrest and apoptosis by&nbsp;dimercaptosuccinic acid modified Fe3O4 magnetic nanoparticles combined with nontoxic concentration of bortezomib and gambogic acid&nbsp;in RPMI-8226 cells

High Efficacy in Hyperthermia-associated with Polyphosphate Magnetic Nanoparticles for Oral Cancer Treatment

Anti-CEA loaded maghemite nanoparticles as a theragnostic device for colorectal cancer

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Product

Resources

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Inducing cell cycle arrest and apoptosis by dimercaptosuccinic acid modified Fe3O4 magnetic nanoparticles combined with nontoxic concentration of bortezomib and gambogic acid in RPMI-8226 cells

Inducing cell cycle arrest and apoptosis by dimercaptosuccinic acid modified Fe3O4 magnetic nanoparticles combined with nontoxic concentration of bortezomib and gambogic acid in RPMI-8226 cells