The effect of different solvents on the ATP/ADP content and growth properties of HeLa cells

Forman, Stanislav; Káš, Jan; Fini, Fabiana; Steinberg, Mark L.; Ruml, Tomáš

doi:10.1002/(sici)1099-0461(1999)13:1<11::aid-jbt2>3.0.co;2-r

Cited by 46 publications

(33 citation statements)

References 19 publications

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“…30,31 Fillers are mixed with the polymer at a given content, and homogeneously distributed in the MONTJOVENT ET AL. composite preform before foaming.…”

Section: Discussionmentioning

confidence: 99%

Biocompatibility of Bioresorbable Poly(L-lactic acid) Composite Scaffolds Obtained by Supercritical Gas Foaming with Human Fetal Bone Cells

et al. 2005

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The aim of this investigation was to test the biocompatibility of three-dimensional bioresorbable foams made of poly(L-lactic acid) (PLA), alone or filled with hydroxyapatite (HA) or ␤-tricalcium phosphate (␤-TCP), with human primary osteoblasts, using a direct contact method. Porous constructs were processed by supercritical gas foaming, after a melt-extrusion of ceramic/polymer mixture. Three neat polymer foams, with pore sizes of 170, 310, and 600 m, and two composite foams, PLA/5 wt% HA and PLA/5 wt% ␤-TCP, were examined over a 4-week culture period. The targeted application is the bone tissue-engineering field. For this purpose, human fetal and adult bone cells were chosen because of their highly osteogenic potential. The association of fetal bone cells and composite scaffold should lead to in vitro bone formation. The polymer and composite foams supported adhesion and intense proliferation of seeded cells, as revealed by scanning electron microscopy. Cell differentiation toward osteoblasts was demonstrated by alkaline phosphatase (ALP) enzymatic activity, ␥-carboxylated Gla-osteocalcin production, and the onset of mineralization. The addition of HA or ␤-TCP resulted in higher ALP enzymatic activity for fetal bone cells and a stronger production of Gla-osteocalcin for adult bone cells.

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“…30,31 Fillers are mixed with the polymer at a given content, and homogeneously distributed in the MONTJOVENT ET AL. composite preform before foaming.…”

Section: Discussionmentioning

confidence: 99%

Biocompatibility of Bioresorbable Poly(L-lactic acid) Composite Scaffolds Obtained by Supercritical Gas Foaming with Human Fetal Bone Cells

et al. 2005

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“…Results were statistically analyzed using an unpaired t -test and indicate that the inhibitor-treated samples have significantly higher caspase-3 activity than their inactive control counterparts. Inactive controls do not differ significantly from the nontreated control, except for DMSO treatment which may by itself introduce changes to in vitro culturing conditions (Forman et al 1999). The p values are given in Table 1.…”

Section: Caspase-3 Activation In Organs Of Corti Exposed To Nf-kb Inhmentioning

confidence: 95%

NF-κB is Required for Survival of Immature Auditory Hair Cells In Vitro

Nagy

Monge

Albinger-Hegyi

et al. 2005

JARO

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Damage to auditory hair cells in the inner ear as a consequence of aging, disease, acoustic trauma, or exposure to ototoxins underlies most cases of hearing impairment. Because the mammalian ear cannot replace damaged hair cells, loss of hearing is irreversible and progressive throughout life. One of the current goals of inner ear biology is to develop therapeutic strategies to prevent hair cell degeneration. Although important progress has been made in discovering factors that mediate hair cell death, very little is known about the molecular pathway(s) that signal survival. Here we considered the role of NF-kB, a ubiquitous transcription factor that plays a major role in the regulation of many apoptosis-and stressrelated genes, in mediating hair cell survival. NF-kB was detected in a constitutively active form in the organ of Corti of 5-day-old rats. Selective inhibition of NF-kB through use of a cell-permeable inhibitory peptide in vitro caused massive degeneration of hair cells within 24 h of inhibitor application. Hair cell death occurred through an apoptotic pathway through activation of caspase-3 and may involve transcriptional down-regulation of the gadd45b gene, an anti-apoptotic NF-kB target. In view of our results, it seems likely that NF-kB may participate in normal hair cell function.Keywords: apoptosis, inhibitor of kB, inner ear, organ of Corti, RelAAbbreviations: a -antibody; bp -base pair; BSAbovine serum albumin; CHAPS -3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate; DMSO -dimethyl sulfoxide; DTT -dithiothreitol; EDTA -ethylendiaminetetraacetic acid; EGTA -ethylenglycol-bis(b-aminoethyl)-N,N,N 0 ,N 0 -tetraacetic acid; FITC -fluorescein isothiocyanate; GADD45b -growth arrest and DNA damage-inducible 45b protein; GAPDH -glyceraldehydes-3-phosphate dehydrogenase protein; HEPES -N-2-Hydroxyethylpiperazine-N 0 -2-ethanesulfonic acid; IB -immunoblotting; IHC -inner hair cell; IkB -inhibitor of kB protein; INH -Synthetic inhibitor of NF-kB protein; INHMut -synthetic inactive control for the inhibitor of NF-kB protein; JNK -c-Jun N-terminal kinase; MIPmultiple image processing; NF-kB -nuclear factor-kB; NT -not treated; OC -organ of Corti; OHC -outer hair cell; p5 -postnatal day 5; PBS -phosphate-buffered saline; PMSF -phenylYmethyl-sulfonyl fluoride; PNT -parthenolide; Rel -reticuloendotheliosis viral oncogene homologue; RT -reverse transcriptase; SG -spiral ganglion; SV -stria vascularis; XIAP -Xlinked inhibitor of apoptosis protein.

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“…Based on reports from in vitro studies, possible toxic effects of these organic solvents are expected (5)(6)(7). Several authors have reported interference of frequently used solvents with cellular based assays (8,9). Therefore, the solvent should be selected to optimize the solubility of poor water-soluble compound without adversely affecting the assay conducts, such as cell growth (10).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic Effects of Some Common Organic Solvents on MCF-7, RAW-264.7 and Human Umbilical Vein Endothelial Cells

Jamalzadeh¹,

Ghafoori²,

Sariri³

et al. 2016

Avicenna J Med Biochem

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Background: Organic solvents are widely used in cell biology experiments. Despite increasing the solubility, they have some moderate toxic effects. Therefore, selecting the appropriate solvent along with the use of suitable concentration insures the accuracy and reliability of experimental results. Objectives: The current study aimed to examine the cytotoxic effects of some organic solvents on various cell models including MCF-7, RAW-264.7 and human umbilical vein endothelial cells (HUVEC). Materials and Methods:To evaluate the cytotoxicity effect of common organic solvents on the MCF-7, RAW-264.7 and HUVEC cells, multi-table tournament (MTT) colorimetric assay, the widely used and validated cytotoxicity test was applied. For this purpose, the selected cells were treated with different concentrations (0, 0.1%, 0.5%, 1%, 1.5%, 2%, 3% and 5% v/v) of four most commonly used organic solvents (acetone, ethanol, dimethyl sulfoxide (DMSO) and dimethylformamide (DMF) and then subjected to MTT experiment. Results: According to the obtained results, the cytotoxicity increased significantly with increasing the concentration of all four solvents compared to that of the control group. Studies with MCF-7, RAW-264.7 and HUVEC suggested that acetone, ethanol and DMSO at concentrations of 0.1% and 0.5%, had little or no toxicity, whereas higher concentrations inhibited the growth of all three cells. Compared with other three solvents, DMF displayed rather greater toxicity. Based on the results, proliferation of MCF-7, RAW-264.7 and HUVEC cells were inhibited by all used organic solvents, dose dependently. Conclusions: Thus, the background experimental error can be reduced remarkably by maximal concentration of 0.5% ethanol, acetone and DMSO and 0.1% DMF in the final treatment medium.

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The effect of different solvents on the ATP/ADP content and growth properties of HeLa cells

Cited by 46 publications

References 19 publications

Biocompatibility of Bioresorbable Poly(L-lactic acid) Composite Scaffolds Obtained by Supercritical Gas Foaming with Human Fetal Bone Cells

Biocompatibility of Bioresorbable Poly(L-lactic acid) Composite Scaffolds Obtained by Supercritical Gas Foaming with Human Fetal Bone Cells

NF-κB is Required for Survival of Immature Auditory Hair Cells In Vitro

Cytotoxic Effects of Some Common Organic Solvents on MCF-7, RAW-264.7 and Human Umbilical Vein Endothelial Cells

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