The effect of different grading equipment on stress levels assessed by catecholamine measurements in Pacific oysters, Crassostrea gigas (Thunberg)

Qu, Yegao; Li, Xiaoxu; Yu, Yang; Vandepeer, Meegan; Babidge, PJ; Clarke, Steven; Bott, Kriston; Li, Hua

doi:10.1016/j.aquaeng.2008.10.003

Cited by 12 publications

(9 citation statements)

References 15 publications

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“…The p-values are for log-rank tests; hpi: hours post-injection extreme than water due to its innate thermal buffering capacity (Song et al 2007, Guo et al 2015, Yang & Peterson 2017. Previous studies have de monstrated that factors associated with vital immune defenses, including stress responses and the integrity of the lysosomal membrane in bivalve molluscs, are significantly altered when exposed to air temperatures higher than the temperature of the water to which they are acclimated (Zhang et al 2006, Qu et al 2009). Therefore, the variable nature of air temperature during periods of prolonged emersion could potentially increase the susceptibility of C. gigas to OsHV-1 (Cheng 1983, Lacoste et al 2001a,b, Li et al 2005, Aladaileh et al 2008, as increased water temperatures in summer months are known to be associated with the onset of POMS outbreaks across both Europe and Australia (Paul-Pont et al 2013b, Petton et al 2013, Pernet et al 2014, Ugalde et al 2018.…”

Section: Discussionmentioning

confidence: 99%

“…Here, prolonged air exposure is defined as emersing the oysters for 24 h, which is significantly longer than the natural range of air exposure experienced in the intertidal environment. Previous investigations have demonstrated that prolonged air exposure of oysters increases catecholamine levels and decreases the pH of oyster tissue, the integrity of the lysozomal membrane and the expression of antioxidants (Zhang et al 2006, Allen & Burnett 2008, Qu et al 2009, Kuchel et al 2012). These physiological changes affect key components of the oyster immune system and therefore are likely to affect the response to OsHV-1 infection.…”

Section: Introductionmentioning

confidence: 99%

“…De Kantzow (2017) demonstrated higher mortality associated with oysters that had been handled for farm management in the week before an OsHV-1 outbreak. These processes involve exposing the oysters to multiple stressors, including both physical trauma (handling and transport) and environmental pressure (altered temperature and air exposure) (Qu et al 2009), and it is not clear which components con-tribute to the higher susceptibility. Prolonged air exposure has known effects on oysters, including limited feeding opportunities, exposure to varying and potentially extreme temperatures and the depletion oxygen (Guo et al 2015).…”

Section: Introductionmentioning

confidence: 99%

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Effect of air exposure, handling stress and imidacloprid on the susceptibility of Crassostrea gigas to Ostreid herpesvirus 1 (OsHV-1)

Oliver¹,

Fuhrmann²,

Hick³

2019

Aquacult. Environ. Interact.

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The emergence of the microvariant genotype of Ostreid herpesvirus 1 (OsHV-1 μVar) has caused mass mortalities of Pacific oysters Crassostrea gigas, resulting in significant economic losses in Europe, New Zealand and Australia. There is variability in the occurrence and severity of disease caused by OsHV-1, with the disease incompletely described by the known complex interactions between host, environment and pathogen. There is a need to evaluate the role of anthropogenic factors on this disease expression due to the number of interactions between humans and oysters. A controlled in vivo laboratory infection model was used to assess changes to the susceptibility of 6 mo old Pacific oysters to OsHV-1 challenge after pre-exposure to combinations of stressors. Pre-exposure of oysters to a concentration of the pesticide imidacloprid consistent with the higher range of environmental contamination in some estuaries had no impact on their survival or OsHV-1 viral load. Oysters pre-exposed to air for 24 h prior to OsHV-1 challenge by cohabitation were more resilient to infection. Moderate physical handling that simulated onfarm handling did not affect survival. This indicates that farm management practices implemented prior to OsHV-1 exposure might not specifically predispose oysters to more severe disease, and more complex confounding factors need to be considered. It is likely that changes in host physiology during emersion provide the host with increased resilience to disease caused by OsHV-1. Continued investigation of the effect of air exposure in the field will aid in validating the results from this laboratory experiment.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of air exposure, handling stress and imidacloprid on the susceptibility of Crassostrea gigas to Ostreid herpesvirus 1 (OsHV-1)

Oliver¹,

Fuhrmann²,

Hick³

2019

Aquacult. Environ. Interact.

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“…Catecholamines, including noradrenaline (NA) and dopamine (DOP), were quantified in CF using HPLC with electrochemical detection as described by Qu, Li, Yu, Vandepeer, Babidge, Clarke, Bott and Li ().…”

Section: Methodsmentioning

confidence: 99%

Immune responses of the sea cucumberApostichopus japonicusto stress in two different transport systems

et al. 2014

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We report the effect of transport stress on the immune responses of the sea cucumber Apostichopus japonicus. The total coelomocytes counts, phagocytosis activity, reactive oxygen species production, acid phosphatase activity, phenoloxidase activity and catecholamine levels were measured in A. japonicus following both damp and water transportation methods. With both transport methods, stress resulted in significant elevation of catecholamine levels. Total coelomocytes counts increased following transportation, whereas phagocytic activity and reactive oxygen species production decreased. Damp transport significantly depressed acid phosphatase and phenoloxidase activity in A. japonicus. However, there were no significant differences in acid phosphatase and phenoloxidase activity after water transportation. These results show that transport stress induces modifications in the immune system of A. japonicus and that damp transport elicited more severe stress than water transport. This study increased understanding of the mechanism of transport stress inducing neuroendocrine responses and immune modification in A. japonicus. The information obtained should be helpful to optimize transport process in sea cucumber culture.

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“…While L-phenylalanine and L-tyrosine are usually assimilated through dietary means, some 75 marine invertebrates have demonstrated an ability to take up free dissolved amino acids from seawater as an alternative or supplementary route (Söylemez et al 2010;Leroy et al 2012). In molluscs, endogenous levels of catecholamines and/or expres-80 sion of their receptors vary depending on developmental stage and age (Kreiling et al 2001), season or reproductive condition (López-Sánchez et al 2009) and exposure to environmental stressors, such as air exposure, mechanical agitation, temperature and sa-85 linity (Chen et al 2008;Qu et al 2009). Thus, catecholamine metabolism is an important pathway linking a wide array of molluscan behaviours and their regulation by external stimuli.…”

Section: Introductionmentioning

confidence: 99%

Putative involvement of adrenergic receptors in regulation of mussel (Perna canaliculus) larval settlement

Young

Sánchez-Lazo

Robertson

2015

Marine Biology Research

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Settlement responses were investigated for mussel (Perna canaliculus) larvae after exposure to catecholamines and their precursor metabolites. Settlement and mortality assays were conducted in Petri plates with chemical treatments (L-phenylalanine, L-tyrosine, L-DOPA, dopamine hydrochloride and epinephrine at various concentrations) and controls. 15The proteinogenic amino acids L-phenylalanine and L-tyrosine both were effective inducers (~65%) of larval settlement at 10 −5 mol L −1 compared with controls (4%). Exposure of larvae to L-DOPA, dopamine and epinephrine resulted in maximum settlement induction (50, 60 and 51%, respectively) at 10 −5 mol L −1 . Larval mortalities were low (comparable to controls) across all concentrations of L-phenylalanine and L-tyrosine treatments whereas high mortalities (>60%) were observed for L-DOPA, dopamine and epinephrine at concentrations ≥ 10 −4 mol L −1. Our results indicate that P. canaliculus 20 larval settlement is under endogenous regulation by a catecholaminergic mechanism. Furthermore, the inductive effects of all tested metabolites in the epinephrine biosynthesis pathway point to a putative involvement of adrenergic-type receptors in regulation of larval settlement in this mussel species.

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The effect of different grading equipment on stress levels assessed by catecholamine measurements in Pacific oysters, Crassostrea gigas (Thunberg)

Cited by 12 publications

References 15 publications

Effect of air exposure, handling stress and imidacloprid on the susceptibility of Crassostrea gigas to Ostreid herpesvirus 1 (OsHV-1)

Effect of air exposure, handling stress and imidacloprid on the susceptibility of Crassostrea gigas to Ostreid herpesvirus 1 (OsHV-1)

Immune responses of the sea cucumberApostichopus japonicusto stress in two different transport systems

Putative involvement of adrenergic receptors in regulation of mussel (Perna canaliculus) larval settlement

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