The effect of dietary supplements on gene expression in mice tissues

Ushakova, Tatyana; Melkonyan, Hovsep; Nikonova, Larisa; Мудрик, Н.Н.; Gogvadze, Vladimir; Zhukova, Aleksandra; Gaziev, A.I.; Bradbury, R.

doi:10.1016/0891-5849(95)02040-3

Cited by 24 publications

(14 citation statements)

References 24 publications

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“…Leakage of intracellular liver enzymes lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) into the surrounding incubation medium was used as a measure of necrotic cell death (Griffin et al, 1996;Hasegawa et al, 1995;James et al, 1993;Parola et al, 1996;Shen et al, 1994;Ushakova et al, 1996;Videla et al, 1995). Activity of LDH was determined by the method of Cabaud and Wroblewski (1958).…”

Section: Analytical Proceduresmentioning

confidence: 99%

Validation of an in vitro model for assessment of androstenedione hepatotoxicity using the rat liver cell line clone‐9

Sahu

Wiesenfeld

Kim

et al. 2008

J of Applied Toxicology

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Androstenedione, a naturally occurring steroid hormone, has been used to enhance athletic performance. Little is known, however, about its hepatotoxicity. Clone-9 cells, a non-transformed epithelial cell line that was originally isolated from normal liver of a 4-week old Sprague-Dawley rat, were used as an in vitro model to assess the hepatotoxic potential of androstenedione. The cultures were treated with androstenedione for 24 h at 37 degrees C in 5% CO(2) at concentrations of 0-100 microg ml(-1). After the treatment period, the cells and the culture supernatants were assayed for markers of cytotoxicity which included: release of liver enzymes, cell viability, cellular double-stranded DNA content, oxidative stress, steatosis, cellular ATP content, caspase-3 activity, the mitochondrial permeability transition and induction of cytochrome P450 activity. Significant concentration-dependent differences from control were observed in some endpoints at medium concentrations of 10 microg ml(-1) and above. These in vitro findings were compared with comparable endpoints obtained from an in vivo study of androstenedione toxicity in female Sprague-Dawley rats. Of the eight endpoints that could be compared between the two studies, only three (lipid accumulation, ATP depletion and P450 activity) appeared to be concordant. This suggests that, under the experimental conditions used, the clone-9 cells were not a good model for androstenedione hepatotoxicity.

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Section: Analytical Proceduresmentioning

confidence: 99%

Validation of an in vitro model for assessment of androstenedione hepatotoxicity using the rat liver cell line clone‐9

Sahu

Wiesenfeld

Kim

et al. 2008

J of Applied Toxicology

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“…If the extent of prooxidant activity of compounds tested is limited, this could suggest that the effects are unlikely to present a problem in biological systems. Dietary antioxidants can exert a number of effects in vivo, such as promoting increased synthesis of endogenous antioxidant defenses by upregulation of their biosynthesis and/or increased gene expression (88). Ushakova et al (88) suggested that "the elevated levels of glutathione in antioxidant-fed mice can be explained by the presence of vitamin E in the dietary supplement, which may preserve the pool of endogenous glutathione."…”

Section: Significance Of the Concept Of Prooxidant Action Of Antioxidmentioning

confidence: 99%

“…Dietary antioxidants can exert a number of effects in vivo, such as promoting increased synthesis of endogenous antioxidant defenses by upregulation of their biosynthesis and/or increased gene expression (88). Ushakova et al (88) suggested that "the elevated levels of glutathione in antioxidant-fed mice can be explained by the presence of vitamin E in the dietary supplement, which may preserve the pool of endogenous glutathione." The authors also demonstrated that dietary supplements can indeed modify gene expression induced by heat shock in vivo as well as protect animal tissues against oxidative stress by enhancing the level of endogenous antioxidants and inducing heat shock protein (hsp) -70 gene expression (88).…”

Section: Significance Of the Concept Of Prooxidant Action Of Antioxidmentioning

confidence: 99%

“…Ushakova et al (88) suggested that "the elevated levels of glutathione in antioxidant-fed mice can be explained by the presence of vitamin E in the dietary supplement, which may preserve the pool of endogenous glutathione." The authors also demonstrated that dietary supplements can indeed modify gene expression induced by heat shock in vivo as well as protect animal tissues against oxidative stress by enhancing the level of endogenous antioxidants and inducing heat shock protein (hsp) -70 gene expression (88). Dietary antioxidants may act directly by scavenging reactive oxygen species in vivo.…”

Section: Significance Of the Concept Of Prooxidant Action Of Antioxidmentioning

confidence: 99%

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Assessment of potential prooxidant and antioxidant actions

Aruoma

1996

J Americ Oil Chem Soc

120

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Suggestions that oxidative stress plays a role in human diseases have led to the proposal that health might be improved by increased dietary intake of antioxidants. Plant-derived antioxidants, such as flavonoids or rosemary extracts, are increasingly proposed as important dietary antioxidant factors, and foods rich in antioxidants are also receiving attention. Before widescale usage of natural and synthetic antioxidants can be suggested, it is necessary to establish the properties of such molecules. Assays for characterizing the potential prooxidant/antioxidant actions of food additives, antioxidant supplements, antioxidant drug molecules, and nutrient components have been developed for this purpose.

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“…ROS are known to induce various cell lesions, e.g., by DNA damage, lipid peroxidation, and oxidative modification of proteins [16]. ROS also modulate the activity of transcription factors, such as the activator protein-1 [17], growth factors such as transforming growth factor-␣ [18], heat shock proteins [19,20], mitogen-activated protein kinases [21], antioxidant enzyme systems [22], and various other genes (COX-2) [23]. An involvement of PPIases in ROS and oxygen defense is likely, but this has not yet been investigated in mammalian embryos.…”

Section: Introductionmentioning

confidence: 99%

Oxygen Stress Increases Prolyl cis/trans Isomerase Activity and Expression of Cyclophilin 18 in Rabbit Blastocysts

Santos¹,

Körber²,

Küllertz³

et al. 2000

Biology of Reproduction

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The peptidyl-prolyl cis/trans isomerase (PPIase) activity and the expression of cyclophilins were studied in 6-day-old rabbit preimplantation embryos cultured under physiological and increased oxygen concentrations of 5% and 20% O(2), respectively. The PPIase activity was completely inhibited by cyclosporin A (CsA). The inhibitor of FK506-binding proteins, rapamycin, had no effect on the PPIase activity, indicating that the PPIase activity in rabbit blastocysts originates from cyclophilins. Using CsA affinity chromatography, only one cyclophilin with a molecular mass of about 17.8 kDa was separated. The cDNA of rabbit cyclophilin was cloned and sequenced. Analysis of the 682-base pair cDNA revealed an open reading frame coding for a polypeptide of 164 amino acid residues with a molecular weight of 17.83 kDa. Homologies of 90% and 96% for the cDNA and amino acid sequence, respectively, to the human CyP18 were found, suggesting that the novel rabbit cyclophilin is a member of the CyP18 family (rabCyP18). The transcription level of rabCyP18 mRNA was 8.3 +/- 0.6 pg in 100 ng total RNA in noncultured blastocysts. In vitro culture with moderate oxygen stress (20% O(2)) resulted in a 1.5-fold increase in rabCyP18 transcription and an increased PPIase activity compared to that of blastocysts cultured with 5% O(2). Increase in transcription rate and PPIase activity by oxygen stress suggests an involvement of CyP18 in oxygen defense in rabbit preimplantation embryos.

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The effect of dietary supplements on gene expression in mice tissues

Cited by 24 publications

References 24 publications

Validation of an in vitro model for assessment of androstenedione hepatotoxicity using the rat liver cell line clone‐9

Validation of an in vitro model for assessment of androstenedione hepatotoxicity using the rat liver cell line clone‐9

Assessment of potential prooxidant and antioxidant actions

Oxygen Stress Increases Prolyl cis/trans Isomerase Activity and Expression of Cyclophilin 18 in Rabbit Blastocysts

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