Background The major storage form of phosphorus in plant-derived feed is presents by insoluble phytates and not digested by animals. Microbial enzymes phytases are able to hydrolyze phytates and are widely used as feed additives in animal nutrition to improving the utilization of phytate-phosphorus in diets and reducing manure phosphorus excretion to the environment. However, no single phytase can perform effectively under various conditions of digestive systems and dietary compositions. That's why there is a constant search and development of new effective feed phytases preparations. The objective of this study was to study the effect of AgpP-P phytase supplementation on productivity, nutrient availability, veterinary-sanitary assessment of meat and serum biochemical parameters of Hubboard broilers. Results Results showed that phytase supplementation increased body weight and absolute weigh gain in broilers, while reducing the total amount of feed consumed by birds (P < 0.05). Phytase improved the availability and absorption of organic nutrients, calcium (Ca) and phosphorus (P). However, no differences were observed for nitrogen (N) (P < 0.05). Phytase supplementation increased Ca and P contents, total protein (TP) level and alanine aminotransferase (ALT) activity, and reduced urea level, aspartate aminotransferase (AST) and alkaline phosphatase (ALP) activities in serum, respectively (P < 0.05). The meat of phytase-fed broilers meets Russian State Standards (GOST) for fresh good-quality meat based on organoleptic, physicochemical and bacterioscopic characteristics. Conclusions The results of present study demonstrated that addition of AgpP-P phytase to the diet of broiler chickens has a positive effect on the nutrient availability and productivity of birds, while reducing the total amount of feed consumed by chickens. Biochemical parameters in serum remained within the physiological norm. The meat of phytase-fed broilers met the Russian State Standards (GOST) for fresh good-quality meat. Thus, it is shown that AgpP-P phytase has the potential for use as a feed additive in poultry farming.