The effect of dietary molybdenum and sulphate on the oestrus cycle and ovulation in ewes after manipulation with exogenous progesterone alone or in combination with FSH and LH

Plessis, Stefan S. du; Niekerk, F.E. Van; Coetzer, W.A.

doi:10.1016/s0921-4488(99)00002-4

Cited by 3 publications

(4 citation statements)

References 16 publications

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“…Therefore, a reduction in the production of androstenedione, as we have reported for TTM doses greater than 5, is likely to limit the production of oestradiol due to substrate limitation. The results of the present study are consistent with an effect of molybdenum-induced copper deficiency on the development of the preovulatory follicle, especially steroid secretion, and would therefore be expected to perturb both behavioural oestrus and ovulation to a varying degree, as has been observed in animals with molybdenum-induced copper responsive subfertility (Phillippo et al 1987, Du Plessis et al 1999a, 1999b. Further, it is well established that ovarian oestradiol plays a key role in controlling gamete transport and in priming the uterus for implantation, so that even if ovulation does occur, fertility rates are likely to be impaired if preovulatory oestradiol production is sub-optimal .…”

Section: Discussionsupporting

confidence: 89%

“…Further, it is well established that ovarian oestradiol plays a key role in controlling gamete transport and in priming the uterus for implantation, so that even if ovulation does occur, fertility rates are likely to be impaired if preovulatory oestradiol production is sub-optimal . Again, this is one of the key symptoms of copper-responsive subfertility (Du Plessis et al 1999a) and further studies are required to examine the effects of molybdenum-induced copper deficiency on the pattern of development and steroidogenic capacity of ovulatory follicles in vivo.…”

Section: Discussionmentioning

confidence: 99%

“…Du Plessis et al (1999a) have also shown that behavioural anoestrus occurs relatively quickly, within 6 weeks, in sheep fed a high molybdenum and sulphur diet, with true anoestrus occurring after a much longer supplementation time in the trial. Du Plessis et al (1999b) also showed that ovaries were reduced in size and have a decreased response to a follicle-stimulating hormone (FSH)-induced superovulation regimen in the molybdenum and sulphur supplemented groups.…”

Section: Introductionmentioning

confidence: 93%

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Effect of copper and thiomolybdates on bovine theca cell differentiation in vitro

Kendall¹,

Marsters²,

Guo³

et al. 2006

Journal of Endocrinology

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Subfertility that will respond to appropriate copper supplementation is a widespread problem in the national dairy herd. The aims of this study were to determine the effect of copper and/or copper chelating thiomolybdates on LH-induced differentiation by looking at the effects on androgen production, steroidogenic enzymes (cytochrome P450 17 -hydroxylase and cytochrome P450 side-chain cleavage) and lysyl oxidase mRNA expression in cultured theca cells maintained under serum-free conditions.The effect of thiomolybdates and copper on LH differentiation was investigated by supplementing (ammonium) tetrathiomolybdate to optimum theca cell culture media at 0-100 µg/ml, copper (chloride) at equimolar concentrations (0-51·6 µg/ml) or equimolar combinations of both media. Lysyl oxidase mRNA expression was investigated with semi-quantitative RT-PCR, whilst expression of cytochrome P450 17 -hydroxylase and cytochrome P450 side-chain cleavage mRNA was examined using real time PCR. Both PCRs used bovine specific primers and cell lysates obtained from bovine theca cells cultured for 6 days and in the presence or absence of the 100 µg/ml dose of thiomolybdate and equimolar copper thiomolybdate.Thiomolybdate depressed androstenedione production in a dose-dependent manner at doses greater than 1 µg/ml at 96 h (P<0·05); doses above 20 µg/ml were all greatly reduced at all time points and at 192 h when related to numbers of cells (P<0·001). Copper alone had no effect at physiological doses, but the use of the equimolar copper thiomolybdate reversed the effect of tetrathiomolybdates on androstenedione production at the 20 µg/ml dose. Thiomolybdate supplementation, with and without copper, had no significant effect on the level of lysyl oxidase or cytochrome P450 side-chain cleavage expression. However, cytochrome P450 17 -hydroxylase expression was significantly increased (P<0·05) by tetrathiomolybdate, possibly due to a local regulatory system.In conclusion, these results demonstrate that thiomolybdates can prevent LH-induced differentiation of bovine theca cells in vitro and that these effects can be ameliorated by copper supplementation. Our results also indicate that it is unlikely that the effects of thiomolybdate are mediated at the transcriptional level and further work is required to determine if thiomolybdate exerts its effects through post-translation processing or some other unrelated mechanism. Overall, these data support the hypothesis that copper responsive subfertility results from perturbation of the normal pattern of ovarian steroidogenesis.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 93%

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Effect of copper and thiomolybdates on bovine theca cell differentiation in vitro

Kendall¹,

Marsters²,

Guo³

et al. 2006

Journal of Endocrinology

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show abstract

“…This is not desirable to consumers. In addition, an excessive dietary supplement of ammonium molybdate (100 mg/kg DM) and sodium sulfate (4.5 g/kg DM) to induce a secondary Cu deficiency resulted in an impaired reproductive performance of ewes (du Plessis et al, 1999).…”

Section: Resultsmentioning

confidence: 99%

Effects of dietary molybdenum, sulfur and zinc on the excretion and tissue accumulation of trace elements in sheep fed palm kernel cake-based diets

Al-Kirshi

Alimon

Ivan

2011

Animal

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Twelve male 8-month-old lambs were used in a 6-month feeding experiment to determine the effects of dietary Mo, Mo 1 S and Zn supplements on the body retention and tissue accumulation of dietary Cu, Zn and Fe. The lambs were divided into four groups of three lambs each and each group was fed ad libitum one of four diets. A control diet was based on palm kernel cake (PKC) and grass hay. Three additional diets were the control supplemented with either Mo or Mo 1 S or Zn. At 3 months of the experiment, feces and urine were collected and sampled for 6 days. At the end of the experiment (6 months), blood was sampled and then the sheep were slaughtered. The liver and kidney were removed and sampled for chemical analysis. In comparison with the control, each dietary supplement decreased (P , 0.05) the Cu concentration in the liver, but only the Mo 1 S supplement decreased it to a safe range of below 350 mg/g dry matter. This was accompanied by the body retention of dietary Cu of 24.6%, 6.7%, 2.5% and 6.5% for the control, Mo, Mo 1 S and Zn treatments, respectively. The blood plasma concentration of Cu was decreased (P , 0.05) by the Zn supplement, but was not affected by other supplements (P . 0.05). It was concluded that from the supplements tested, only Mo 1 S appeared to be effective in reducing the retention and liver accumulation of the dietary Cu to prevent chronic Cu toxicity in sheep fed PKC-based diets.

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Dual effects of molybdenum on mouse oocyte quality and ovarian oxidative stress

Zhang

Liu

Chen

et al. 2013

Systems Biology in Reproductive Medicine

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A sub-acute toxicity test was performed to investigate the effects of molybdenum (Mo) on ovarian function. ICR adult female mice were exposed to Mo by free access to distilled water containing the Mo at 5, 10, 20, and 40 mg/L for 14 days. Compared to the control group, M II oocyte morphology, ovary index, and ovulation improved within the 5 mg/L Mo group, but were negatively affected by Mo at 40 mg/L. Morphologically abnormal ovarian mitochondria were observed at ≥ 20 mg/L. These alterations accompanied the changes in superoxide dismutase (SOD), glutathione peroxidise (GPx), and malondialdehyde (MDA) levels in ovaries. In conclusion, Mo affects oocyte quality possibly through regulating ovarian oxidative stress in a dose-dependent manner. It appears that Mo may improve ovarian function at a suitable concentration, which might be a candidate for the treatment of female infertility.

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The effect of dietary molybdenum and sulphate on the oestrus cycle and ovulation in ewes after manipulation with exogenous progesterone alone or in combination with FSH and LH

Cited by 3 publications

References 16 publications

Effect of copper and thiomolybdates on bovine theca cell differentiation in vitro

Effect of copper and thiomolybdates on bovine theca cell differentiation in vitro

Effects of dietary molybdenum, sulfur and zinc on the excretion and tissue accumulation of trace elements in sheep fed palm kernel cake-based diets

Dual effects of molybdenum on mouse oocyte quality and ovarian oxidative stress

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