The effect of diburyryl cAMP on sodium uptake by isolated perfused gills of Callinectes sapidus

Lohrmann, Donna M.; Kamemoto, Fred I.

doi:10.1016/0016-6480(87)90177-8

Cited by 44 publications

(19 citation statements)

References 22 publications

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“…In C. sapidus, Kamemoto and Oyama (1985) demonstrated that extracts from pericardial organs stimulated Na ϩ uptake when applied to gill tissue. Also, DA and octopamine elevated cAMP levels and applications of dibutytyl cAMP elevated Na ϩ uptake in gill tissue, suggesting that the amines act through this second messenger pathway to increase Na ϩ uptake (Lohrmann and Kamemoto, 1987). That DA and cAMP act as first and second messengers, respectively, to increase Na ϩ uptake was supported by subsequent research using gill tissue from C. maenas (Sommer and Mantel 1988), the purple shore crab, Leptograpsus variegatus (Morris and Edwards, 1995), and the Chinese mitten crab, Eriochier sinesis (Mo et al, 1998).…”

Section: Hormone Release and Ionic Regulationmentioning

confidence: 94%

Dopamine in crayfish and other crustaceans: Distribution in the central nervous system and physiological functions

Tierney

Kim

Abrams

2003

Microscopy Res & Technique

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Dopamine is widely distributed in the crustacean nervous system and has a diverse array of physiological effects. Immunocytochemical studies of several species have shown that dopamine- and/or tyrosine hydroxylase-containing cells occur in all ganglia of the central nervous system and that processes from some of these cells link ganglia of the ventral nerve cord. This study describes the distribution of tyrosine hydroxylase-containing cells in the central nervous system of a crayfish (Orconectes rusticus) and compares this information to available data from other species. The distribution of tyrosine hydroxylase (an enzyme in the synthetic pathway between tyrosine and dopamine) in O. rusticus is similar to that reported for marine species. However, differences were observed in the number of neurons in some ganglia and in the axonal projections of the L cell, which were more extensive in O. rusticus than in other species studied thus far. We also review the physiological effects of dopamine in crayfish and other crustaceans, focusing on the amine's actions in the endocrine, cardiovascular, and nervous systems, and on behavior when injected into freely-moving animals.

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Section: Hormone Release and Ionic Regulationmentioning

confidence: 94%

Dopamine in crayfish and other crustaceans: Distribution in the central nervous system and physiological functions

Tierney

Kim

Abrams

2003

Microscopy Res & Technique

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“…In early works, PO extract, dopamine (DA), serotonin, octopamine and hemolymph from seawater acclimated crabs have been injected into whole animals or tested in isolated perfused gills of the blue crab Callinectes sapidus and the Chinese crab Eriocheir sinensis, resulting in increased Na + influx and intracellular cyclic adenosine 3Ј-5Ј monophospate (cAMP) content (Kamemoto, and Oyama, 1985;Lohrmann and Kamemoto, 1987;Sommer and Mantel, 1988;Sommer and Mantel, 1991;Bianchini and Gilles, 1990). The activity of gill Na + /K + -ATPase was also tested after injection of hemolymph from 30% seawater crabs (Savage and Robinson, 1983), injection of DA and PO extract (Sommer and Mantel, 1988), injection of DA or dibutyryl-cAMP (Morris and Edwards, 1995) and after incubation of gills with cAMP (Mo et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Dopaminergic regulation of ion transport in gills of the euryhaline semiterrestrial crabChasmagnathus granulatus: interaction between D1- and D2-like receptors

Genovese

Senek

Ortiz

et al. 2006

Journal of Experimental Biology

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SUMMARY The effects of dopamine (DA) and dopaminergic agonists and antagonists on ion transport were studied in isolated perfused gills of the crab Chasmagnathus granulatus. DA applied under steady state conditions(perfusion with hemolymph-like saline) produced a transient increase of the transepithelial potential difference (Vte) from 2.2±0.2 to 4.8±0.3 mV, describing an initial cAMP-dependent stimulating phase followed by an inhibitory phase. Spiperone and domperidone(antagonists of D2-like DA receptors in vertebrates) completely blocked the response to DA, while the D1-like antagonist SCH23390 blocked only the inhibitory phase. Theophylline (phosphodiesterase inhibitor) and okadaic acid(protein phosphatases PP1 and PP2A inhibitor) were also able to block the inhibitory phase, suggesting that it depends on adenylyl cyclase inhibition and on protein phosphatases. When the gills were perfused with hypo-osmotic solution, or with the adenylyl cyclase activator forskolin, Vte was increased several-fold. DA applied under these stimulated conditions partially reversed the Vte increase by 54% and 25%, respectively. Similarly, the D1-like agonist, fenoldopam,produced a 33% reduction in the stimulated Vte. We propose that, in C. granulatus gills, DA stimulates adenylyl cyclase and therefore ion transport through D1-like receptors linked to a Gs protein,although they respond to antagonists that interact with D2-like receptors in vertebrates. The inhibitory phase seems to be mediated by D2-like receptors linked to a Gi/o protein, which inhibits adenylyl cyclase, although these receptors can be activated or blocked by agonists or antagonists that interact with D1-like receptors in vertebrates and insects.

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“…The upregulation by serotonin in red crabs is similar in principle to that in osmoregulating marine brachyuran crabs (Morris, 2001 for review) but differs in detail. A dopaminergic, cAMP-mediated upregulation of branchial ion pumping appears ubiquitous in the aquatic brachyuran species (Lohrmann and Kamemoto, 1987;Mantel, 1988, 1991;Bianchini and Gilles, 1990;Detaille et al, 1992;Morris and Edwards, 1995). Other than the work of Trausch et al (1989) on Eriocheir sinesis, there has been little suggestion of a role for serotonin but this too was dependent on a cAMP-activated protein kinase.…”

Section: Branchial Mechanismsmentioning

confidence: 99%

“…Biogenic amines, including octopamine and dopamine, have been linked to an elevation of cAMP in increasing the Na uptake in diverse marine species (e.g. Kamemoto and Oyama, 1985;Lohrmann and Kamemoto, 1987;Mantel, 1988, 1991). In Chinese mitten crab Eriocheir sinesis, bioamines promote protein phosphorylation via a cAMP-dependent protein kinase in the gill tissue (Trausch et al, 1989) and stimulate Na flux (Bianchini and Gilles, 1990;Detaille et al, 1992;Mo et al, 1998).…”

mentioning

confidence: 99%

Regulation of urine reprocessing in the maintenance of sodium and water balance in the terrestrial Christmas Island red crabGecarcoidea natalisinvestigated under field conditions

Morris

Ahern

2003

Journal of Experimental Biology

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SUMMARYLand crabs produce isosmotic urine but reduce salt loss by reabsorbing salt via the gills to produce a dilute excretory fluid (P). This branchial salt reclamation is regulated in response to changes in dietary salt availability. The regulation of branchial Na reabsorption and osmotic status was investigated in the terrestrial crab Gecarcoidea natalis on Christmas Island. Confinement within field enclosures had no general effect on salt and water balance compared with crabs free in the rainforest but there were seasonal effects. Extracellular fluid volume was 27.9% body mass during the wet season but only 22.7% in the dry season. Urine production was 53 ml kg-1 day-1 in the dry season but 111 ml kg-1day-1 in the wet season, while water flux rates were 140 ml kg-1 day-1 and 280 ml kg-1 day-1,respectively. Serotonin but not dopamine increased urine production by at least 16% but only during the dry season when rates were seasonally lowered. Crabs acclimated to drinking 50% seawater increased haemolymph osmotic pressure and downregulated branchial reabsorption of salt. Net Na flux(Jnet) and unidirectional Na influx(Jin) were investigated in branchial perfusion experiments. In red crabs acclimated to drinking freshwater, Jin, Jnet and the activity of the Na+/K+-ATPase were increased by serotonin, indicating that the increase of sodium absorption was due to a stimulation of the ATPase. Red crabs drinking 50% seawater reduced Jnet primarily due to increased passive loss (Jout), since both Jin and Na+/K+-ATPase were unchanged. Serotonin and dopamine abolished the increased diffusive loss and re-established Jnet with no change in Jin. G. natalis exhibits different regulatory systems. Branchial salt uptake can be adjusted via the leak component when adequate salt is available but also by stimulated active uptake under diluting conditions. The gills are important sites of ion pumping in euryhaline aquatic crabs, and the upregulation of Jnet in red crabs is reminiscent of that in marine crabs. Serotonergic stimulation of branchial uptake, independent of cAMP, and hormonally modulated ion leakage are presently unique to terrestrial species.

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The effect of diburyryl cAMP on sodium uptake by isolated perfused gills of Callinectes sapidus

Cited by 44 publications

References 22 publications

Dopamine in crayfish and other crustaceans: Distribution in the central nervous system and physiological functions

Dopamine in crayfish and other crustaceans: Distribution in the central nervous system and physiological functions

Dopaminergic regulation of ion transport in gills of the euryhaline semiterrestrial crabChasmagnathus granulatus: interaction between D1- and D2-like receptors

Regulation of urine reprocessing in the maintenance of sodium and water balance in the terrestrial Christmas Island red crabGecarcoidea natalisinvestigated under field conditions

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