The effect of dialysis membranes on lipid peroxidation in the erythrocytes of patients with terminal renal insufficiency

Abstract

The content of malonic dialdehyde is shown to be significantly increased in the erythrocytes of patients as compared to the control. During hemodialysis using a regenerated cellulose membrane, the level of malonic dialdehyde reliably increased after 30 min of treatment (p<0.05) and dropped to the initial level following 180 min of hemodialysis. The level of membrane malonic dialdehyde on the erythrocytes remained at the pre-dialysis level at the 30th and 180th min of hemodialysis when a polysulfone membrane was used. It may be assumed that the activation of lipid peroxidation is due to the interaction of cells, with the dialysis membrane and can be considered as one of the markers of biocompatibility. Key Words: terminal renal insufficiency; erythrocytes; dialysis membrane; lipid peroxidationAnemia is a severe complication of terminal renal insufficiency (TRI). The TRI-associated increase in erythrocyte damage is due to various factors, connected both with the changes of structural-functional properties of the erythrocyte membrane and with the uremia-related disturbance of the general metabolism [3,9]. The data obtained by us and many other researchers present evidence that one of the causes of structural alterations of the erythrocyte membrane and increased hemolysis in TRI patients treated with hemodialysis (HD) is an intensification of lipid peroxidation (LPO) in the erythrocyte membrane [1,7,15]. LPO activation in the erythrocyte membrane in uremic patients treated with HD appears to be related to the effect of oxygen free radicals [8] Department of General Pathology and Pathophysiology, and Department of Nephrology, Russian Medical Academy of Postgraduate Education, Moscow that can be generated in excess by direct contact of neutrophils with the membrane or via C3a and C5a complement fra~o-nents. Practically no increase of oxidative metabolism in the neutrophils was noted when polyacrylonitryl and polysulfone membranes were used during HD [11,13]. These membranes seem to differ from cuprophan membrane in that they absorb the blood proteins in their specific way and possess low complement-activating capacity.The goal of this work was a comparative study of different dialysis membranes regarding their effect on LPO in the erythrocyte membrane of TRI patients during H D.

The use of porous polysulfone as a new material for intraorbital implantation

“…Polysulfone has been used for the preparation of dialysis membranes [1,5] and to enlarge the crista of the alveolar process in animals [4]. We have been unable to find any published data on the use of this material for orbital implants.…”