The effect of DGAT1 polymorphism on milk production traits in dairycows depending on environmental temperature

Komisarek, Jolanta; Kolenda, Mateusz

doi:10.3906/vet-1508-7

Cited by 7 publications

(5 citation statements)

References 15 publications

(22 reference statements)

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“…The high proportion of the lysine variant K232 observed in the current study might contribute to the higher fat content in native cattle in Ethiopia and other Bos indicus breeds than in HF cross cattle. The frequency of the lysine variant K232 in the investigated Boran * HF cross cattle is similar to that found in studies of other HF populations or their crosses, which have reported a range from 0.21 to 0.63 (Ahani et al, 2015;Akyüz et al, 2015;Komisarek and Kolenda, 2016;Bhat et al, 2017;Li et al, 2020;Krovvidi et al, 2021). The differences in the frequency of the lysine variant K232 among breeds might result from different breeding goals and the considerable Holstein contribution to the blood level of Boran * HF cross cattle.…”

Section: Allele and Genotype Frequencies Of The K232a Protein Variantsupporting

confidence: 86%

Effect of the DGAT1 K232A mutation and breed on milk traits in cattle populations of Ethiopia

2023

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Non-conservative K232A substitution in the diacylglycerol acyl-CoA acyltransferase 1 (DGAT1) gene has been reported to explain variation in milk traits in cattle. The objective of this study was to estimate allele and genotype frequencies and to assess associations between K232A variants and milk yield and composition in cattle populations of Ethiopia. Blood samples for genomic DNA extraction and milk samples for analysis of milk components were collected from 92 randomly selected cattle of five Ethiopian breeds. Gene-specific primers were used to amplify 278 bp of the exon 8 region of DGAT1. Allele and genotype frequencies were calculated using Power Marker, and the GLM function of SAS software was used to assess the associations of detected genetic variation with milk traits. Boran * Holstein Friesian (HF) crosses produced a higher daily milk yield than the other breeds (p< 0.05), whereas the Boran and Begait breeds produced milk with higher fat and protein contents (p< 0.05), and Horro produced milk with a higher content of lactose, than the other breeds studied (p< 0.05). Alleles K and A and genotypes AA, KA, and KK were detected. The frequency of K232 ranged from 0.50 in Boran * HF crosses to 0.97 in the Horro population. The frequency of the KK and KA genotypes in the zebu population ranged from 0.50 to 0.94 and from 0.03 to 0.50, respectively. The AA genotype was associated with higher milk yield in Boran * HF crosses, whereas the KA genotype was associated with higher milk yield in the zebu populations (p< 0.05). The fat and lactose contents of milk produced with the KA genotype were lower than those of milk produced with the KK genotype in all genetic groups (p< 0.05). Substitution of one copy of the K allele led to a significant (p< 0.05) increase in fat content, of up to 0.81%, a decrease in daily milk yield of up to 3 L, and a decrease in lactose content of 0.58% in the sampled populations. The association study confirmed that the DGAT1 K232A marker had significant effects on daily milk yield, milk fat and lactose contents in the investigated cattle. These results suggested that the DGAT1 K232A marker may be utilized to accelerate future molecular breeding of dairy cattle, tropical zebu, their crossbreeds, after validation in a larger population.

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Section: Allele and Genotype Frequencies Of The K232a Protein Variantsupporting

confidence: 86%

Effect of the DGAT1 K232A mutation and breed on milk traits in cattle populations of Ethiopia

2023

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“…However, some of the milk production traits (milk protein yield and milk yield) were not constant throughout the lactation and slightly decreased with change in environmental temperature (20-28 • C). The more prominent effect of DGAT K232A in Polish Holstein-Friesian was not observed because the temperature was moderate (5 • C−28 • C) in this study (75). Shahzad et al noticed the low hepatic expression of DGAT1 in periparturient dairy cattle during summer compared with spring (76).…”

Section: Dgat1 K232a Association With Milk Production In Cattlementioning

confidence: 49%

Association of DGAT1 With Cattle, Buffalo, Goat, and Sheep Milk and Meat Production Traits

Khan

et al. 2021

Front. Vet. Sci.

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Milk fatty acids are essential for many dairy product productions, while intramuscular fat (IMF) is associated with the quality of meat. The triacylglycerols (TAGs) are the major components of IMF and milk fat. Therefore, understanding the polymorphisms and genes linked to fat synthesis is important for animal production. Identifying quantitative trait loci (QTLs) and genes associated with milk and meat production traits has been the objective of various mapping studies in the last decade. Consistently, the QTLs on chromosomes 14, 15, and 9 have been found to be associated with milk and meat production traits in cattle, goat, and buffalo and sheep, respectively. Diacylglycerol O-acyltransferase 1 (DGAT1) gene has been reported on chromosomes 14, 15, and 9 in cattle, goat, and buffalo and sheep, respectively. Being a key role in fat metabolism and TAG synthesis, the DGAT1 has obtained considerable attention especially in animal milk production. In addition to milk production, DGAT1 has also been a subject of interest in animal meat production. Several polymorphisms have been documented in DGAT1 in various animal species including cattle, buffalo, goat, and sheep for their association with milk production traits. In addition, the DGAT1 has also been studied for their role in meat production traits in cattle, sheep, and goat. However, very limited studies have been conducted in cattle for association of DGAT1 with meat production traits in cattle. Moreover, not a single study reported the association of DGAT1 with meat production traits in buffalo; thus, further studies are warranted to fulfill this huge gap. Keeping in view the important role of DGAT1 in animal production, the current review article was designed to highlight the major development and new insights on DGAT1 effect on milk and meat production traits in cattle, buffalo, sheep, and goat. Moreover, we have also highlighted the possible future contributions of DGAT1 for the studied species.

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“…Environmental conditions, such as season, nutrition, and management, are also variables that can modify gene expression of most of the traits of interest. Recently, studies revealed that polymorphisms in diacylglycerol O-acyltransferase 1 (DGAT1) and stearoyl-CoA desaturase 1 (SCD1) genes showed different effects on milk production traits and fatty acid (FA) composition according to the season and temperature (Duchemin et al, 2013;Komisarek and Kolenda, 2016). In addition, a joint GWAS of the Chinese and Danish Holstein populations showed potential genotype by environment Genome-wide association study for milk production traits in a Brazilian Holstein population interaction for milk FA traits (Li et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Genome-wide association study for milk production traits in a Brazilian Holstein population

Iung

Petrini

Ramírez-Díaz

et al. 2019

Journal of Dairy Science

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Advances in the molecular area of selection have expanded knowledge of the genetic architecture of complex traits through genome-wide association studies (GWAS). Several GWAS have been performed so far, but confirming these results is not always possible due to several factors, including environmental conditions. Thus, our objective was to identify genomic regions associated with traditional milk production traits, including milk yield, somatic cell score, fat, protein and lactose percentages, and fatty acid composition in a Holstein cattle population producing under tropical conditions. For this, 75,228 phenotypic records from 5,981 cows and genotypic data of 56,256 SNP from 1,067 cows were used in a weighted single-step GWAS. A total of 46 windows of 10 SNP explaining more than 1% of the genetic variance across 10 Bos taurus autosomes (BTA) harbored well-known and novel genes. The MGST1 (BTA5), ABCG2 (BTA6), DGAT1 (BTA14), and PAEP (BTA11) genes were confirmed within some of the regions identified in our study. Potential novel genes involved in tissue damage and repair of the mammary gland (COL18A1), immune response (LTTC19), glucose homeostasis (SLC37A1), synthesis of unsaturated fatty acids (LTBP1), and sugar transport (SLC37A1 and MFSD4A) were found for milk yield, somatic cell score, fat percentage, and fatty acid composition. Our findings may assist genomic selection by using these regions to design a customized SNP array to improve milk production traits on farms with similar environmental conditions.

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The effect of DGAT1 polymorphism on milk production traits in dairycows depending on environmental temperature

Cited by 7 publications

References 15 publications

Effect of the DGAT1 K232A mutation and breed on milk traits in cattle populations of Ethiopia

Effect of the DGAT1 K232A mutation and breed on milk traits in cattle populations of Ethiopia

Association of DGAT1 With Cattle, Buffalo, Goat, and Sheep Milk and Meat Production Traits

Genome-wide association study for milk production traits in a Brazilian Holstein population

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