The effect of dextrin–rhEGF on the healing of full-thickness, excisional wounds in the (db/db) diabetic mouse

Hardwicke, Joseph; Hart, Jeff; Bell, Andrea; Duncan, Ruth; Thomas, David W.; Moseley, Ryan

doi:10.1016/j.jconrel.2011.03.016

Cited by 82 publications

(60 citation statements)

References 40 publications

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“…These findings confirm the following: (i) microencapsulation into a PLGA-Alginate MS protects the growth factor from the wound environment, making it less vulnerable to protease inactivation and oxidative stress present in the wound area (Chen, et al 1997;Hardwicke, et al 2011). Moreover, it is likely that rhEGF released from EGF MS is still active 11 days after wounding.…”

Section: Discussionsupporting

confidence: 78%

rhEGF-loaded PLGA-Alginate microspheres enhance the healing of full-thickness excisional wounds in diabetised Wistar rats

Gainza

Aguirre

Pedraz

et al. 2013

European Journal of Pharmaceutical Sciences

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Diabetic foot ulcers (DFUs) represent a major clinical challenge in the ageing population. To address this problem, rhEGF-loaded Poly-Lactic-co-Glycolic-Acid (PLGA)-Alginate microspheres (MS) were prepared by a modified w/o/w-doubleemulsion/solvent evaporation method. Different formulations were evaluated with the aim of optimising MSs properties by adding NaCl to the surfactant solution and/or the solvent removal phase and adding alginate as a second polymer. The characterization of the developed MS showed that alginate incorporation increased the encapsulation efficiency (EE) and NaCl besides increasing the EE also became the particle surface smooth and regular. Once the MS were optimised, the target loading of rhEGF was increased to 1% (PLGA-Alginate MS), and particles were sterilised by gamma radiation to provide the correct dosage for in vivo studies. In vitro cell culture assays demonstrated that neither the microencapsulation nor the sterilisation process affected rhEGF bioactivity or rhEGF wound contraction. Finally, the MS were evaluated in vivo for treatment of the full-thickness wound model in diabetised Wistar rats. rhEGF MS treated animals showed a statistically significant decrease of the wound area by days 7 and 11, a complete re-epithelisation by day 11 and an earlier resolution of the inflammatory process. Overall, these findings demonstrate the promising potential of rhEGF-loaded MS (PLGA-Alginate MS) to promote faster and more effective wound healing, and suggest its possible application in DFU treatment.

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Section: Discussionsupporting

confidence: 78%

rhEGF-loaded PLGA-Alginate microspheres enhance the healing of full-thickness excisional wounds in diabetised Wistar rats

Gainza

Aguirre

Pedraz

et al. 2013

European Journal of Pharmaceutical Sciences

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“…MMP-9 was selected since significantly elevated levels in MMP-9 have been reported in chronic compared with acute wound fluids, suggesting a direct correlation with the clinical wound state. 42 Further to the study in vitro, full thickness wounds were created in db/db diabetic mice as well-accepted animal models in wound healing research, 43,44 and treated with either AC hydrogel or abovementioned wound dressing gold standards.…”

mentioning

confidence: 99%

Protease-sensitive atelocollagen hydrogels promote healing in a diabetic wound model

et al. 2016

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“…Histological scorings of granulation tissue formation and wound maturity were performed using a 5-point visual scoring scale by two independent investigators in a blinded fashion as per the previously reported method. 26 The extent of collagen deposition in the wound tissue was studied by Masson's trichrome (MT) stain. And semi-quantitative estimation of collagen was carried out by measuring the blue area percentage of the wound tissue by using Image Pro Plus 6.0 software (Media Cybernetics, Rockville, MD, USA).…”

Section: Histopathological and Immunofluorescence Analysismentioning

confidence: 99%

EGF and curcumin co-encapsulated nanoparticle/hydrogel system as potent skin regeneration agent

et al. 2016

IJN

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Wound healing is a complex multifactorial process that relies on coordinated signaling molecules to succeed. Epidermal growth factor (EGF) is a mitogenic polypeptide that stimulates wound repair; however, precise control over its application is necessary to reduce the side effects and achieve desired therapeutic benefits. Moreover, the extensive oxidative stress during the wound healing process generally inhibits repair of the injured tissues. Topical applications of antioxidants like curcumin (Cur) could protect tissues from oxidative damage and significantly improve tissue remodeling. To achieve much accelerated wound healing effects, we designed a novel dual drug co-loaded in situ gel-forming nanoparticle/hydrogel system (EGF-Cur-NP/H) which acted not only as a supportive matrix for the regenerative tissue, but also as a sustained drug depot for EGF and Cur. In the established excisional full-thickness wound model, EGF-Cur-NP/H treatment significantly enhanced wound closure through increasing granulation tissue formation, collagen deposition, and angiogenesis, relative to normal saline, nanoparticle/hydrogel (NP/H), Cur-NP/H, and EGF-NP/H treated groups. In conclusion, this study provides a biocompatible in situ gel-forming system for efficient topical application of EGF and Cur in the landscape of tissue repair.

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The effect of dextrin–rhEGF on the healing of full-thickness, excisional wounds in the (db/db) diabetic mouse

Cited by 82 publications

References 40 publications

rhEGF-loaded PLGA-Alginate microspheres enhance the healing of full-thickness excisional wounds in diabetised Wistar rats

rhEGF-loaded PLGA-Alginate microspheres enhance the healing of full-thickness excisional wounds in diabetised Wistar rats

Protease-sensitive atelocollagen hydrogels promote healing in a diabetic wound model

EGF and curcumin co-encapsulated nanoparticle/hydrogel system as potent skin regeneration agent

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