The Effect of Depotocin inj. Spofa on Concentrations of Cholesterol, Total Lipids, Progesterone and on the Conception Rate in Postparturient Ewes

Abstract

The effect of Depotocin inj. Spofa on concentrations of cholesterol, total lipids and progesterone with subsequent synchronization effect on the conception of ewes was studied. A total of 19 ewes were included in the experiment; 10 animals served as a control, and 9 sheep were treated with Depotocin inj. Spofa 2 -0 -methylthyrosin (deamino -1 -carba) oxytocinum at a dose of 2 x 0.07 mg, 24 and 72 h after lambing, respectively. Blood samples were obtained from v.jugularis at 36 h post partum, and on days 4, 7, 14, 17,21,25,34,42 and 51 after lambing. Agelin vaginal tampons were inserted to sheep of both groups after day 51 (20 mg of chlorsuperlutine). Ten days later, the tampons were removed and the animals were injected with 500 I. U. of PMSG. The expected date of parturition was calculated from the date of mating. Cholesterol and total lipids were determined using BIO-LA tests (LACHEMA, Bmo) and the concentrations of progesterone were determined by the radioimunological method, using kits from 0RJvr, Ko §ice. Cholesterol concentrations showed no significant differences between the control and experimental groups (P > 0.05). The concentrations of total lipids oscillated in the experimental group from day 25 post partum at non-significantly higher values in comparison with the control group by the end of the period studied (1.78 ± 0.48 to 2.45 ± 0.35 g . 1. 1 ). In the experimental group, the levels of progesterone increased slowly on days 34 and 51 (0.5 ± 0.8 to 0.8 ± 1.3 nmol.l· I ). In other sampling days, its values were under the level of sensitivity of the trial. In conclusion, lambing after the oestrus induction on day 51 post partum showed that a 100% lambing with birth of 16 lambs was achieved in the experimental group whereas in the control group only 5 out of 10 mated animals lambed. , ewe, Depotocin, total lipids, cholesterol, progesterone Puerperium length and course in ewes are extremely important from the point of view of lamb meat and lamb broilers production. In our previous studies (KrajniUlkova 1990;Krajnicakova et al. 1991Krajnicakova et al. , 1992, the attention was paid to the physiological course of puerperium and medicamentous influencing of postpartal reparative processes on the reproductive apparatus of sheep following repeated administration of Depotocin inj. Spofa in early puerperium; the authors recorded a 64.3% decrease in the mass of uterine body and uterine horns as early as on day 7 of the period under study. The effect of repeated administration of Depotocin inj. Spofa on the dynamics of concentration changes of thyroxine, triiodothyronine and ovarian steroids was studied by Be k e 0 v a et al. (1992). The authors revealed that the administered carbetocin induces changes in the synthesis and production of both thyroid and steroid hormones through a direct or indirect effect. PuerperiumOxytocin, in addition to its uterotonic and luteolytic effect, acts as an ovarian factor in the local interfollicular regulation of steroidogenesis and as a modulator of uterine secretion of pro...