The effect of delmopinol on glucosyltransferase adsorbed on to saliva-coated hydroxyapatite

Steinberg, Doron; Beeman, Diane K.; Bowen, Willis H.

doi:10.1016/0003-9969(92)90150-7

Cited by 40 publications

(32 citation statements)

References 22 publications

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“…Anoth er explanation is that milk may bind to sucrose, rendering the substrate inaccessible to the GTF enzyme. Similar ef fects on adsorbed GTF activity have been shown in our lab oratory with the antiplaque agent delmopinol [Steinberg et al, 1992], The exact mechanism by which caseins may decrease the incidence of dental caries in rats has not been described. Previous studies in our laboratory and in other laboratories suggest that caseins modulate the adherence of S. mutans to sHA : Nceser et al, 1994VaccaSmith et al.…”

Section: Discussionsupporting

confidence: 56%

The Effect of Milk and kappa Casein on Streptococcal Glucosyltransferase

Vacca-Smith

Bowen

1995

Caries Res

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Many dietary components such as carbohydrates, lipids and proteins may be incorporated into the salivary pellicle and thus may affect glucosyltransferase (GTF) activity on pellicle surfaces. The effect of milk on streptococcal GTF activity was determined. Milk, when coated onto buffer-coated hydroxyapatite or saliva-coated hydroxyapatite (sHA), reduced the subsequent adsorption of GTF onto the surfaces. Milk also reduced the expression of enzymatic activity of GTF adsorbed onto sHA. χ-Casein, when present on the surface of sHA, reduced the adsorption of GTF activity onto sHA, resulting in reduced glucan formation. α-Casein had no effect on the adsorption of GTF onto sHA or on subsequent glucan formation. Both milk and χ-casein reduced activity of the enzyme in solution. The presence of milk and χ-casein fractions on the surface of sHA and in solution with GTF can clearly modulate glucan formation in vitro.

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Section: Discussionsupporting

confidence: 56%

The Effect of Milk and kappa Casein on Streptococcal Glucosyltransferase

Vacca-Smith

Bowen

1995

Caries Res

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“…These changes are due to transformation in the molecular conformation, and other alterations in the immediate microenvironment of the adsorbed enzyme. Also Steinberg et al [1992] and Vacca-Smith and Bowen [1996] have shown that GTFs demonstrate different susceptibility properties to antienzymatic agents in solution and on surfaces.…”

Section: Tested Agentmentioning

confidence: 99%

“…Stimulated whole saliva was collected on the morning of the experiment, as described by Steinberg et al [1992]. A healthy male donor, aged 29 years, was the single source of the salivary samples, in order to eliminate sample variations.…”

Section: Experimental Dental Plaque Modelmentioning

confidence: 99%

“…GTF was prepared as described by Schilling and Bowen [1988] and Steinberg et al [1992]; 200 µl of GTF solution was added to a test tube containing 200 µl of reaction solution consisting of 200 mM sucrose, 20 µM dextran 9000 (Sigma), and 0.075 µCi/reaction of [Uglucosyl 14 C]-sucrose (ARC). One hundred microliters of the active agent (NaF, AmF or AmCl at a concentration range of 0.0005-5 mM) was added to the test tubes.…”

Section: Effect Of the Tested Agents On The Activity Of Gtf Effect Ofmentioning

confidence: 99%

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The Anticariogenic Effect of Amine Fluorides on <i>Streptococcus sobrinus</i> and Glucosyltransferase in Biofilms

Shani

Friedman

Steinberg³

2000

Caries Res

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Dental caries is a chronic infectious disease caused by the accumulation of bacterial plaque (biofilm) on tooth surfaces. Antibacterial agents, in addition to other preventive measures, can control dental plaque accumulation. Amine fluorides (AmF) are known anticaries agents for over 30 years. The purpose of our study was to assess the adsorption and desorption of AmF to experimental dental biofilm and to evaluate the effect of AmF on Streptococcus sobrinus 6715 and glucosyltransferase (GTF) activity in experimental dental biofilms. The experimental plaque model used in this study consists of hydroxyapatite beads coated with human saliva (sHA), followed by adsorption of S. sobrinus and synthesis of in situ polysaccharides. Our results show that the viability of S. sobrinus in biofilm decreased as the concentration of AmF and chlorhexidine (CHX) increased. The concentration of AmF and CHX required to kill S. sobrinus adherent to sHA is about 100 times greater than the concentration required to kill the same amount of planktonic bacteria. Adsorption of AmF to surfaces was more than 90% and the desorption of AmF from our experimental model was limited. Pre–adsorption of AmF on the surface increased adhesion of S. sobrinus but also resulted in surface killing of the adsorbed bacteria. At low concentrations AmF increased GTF activity in solution by about 10%, but at concentrations above 0.1 mM it inhibited GTF activity. Inhibition of GTF on the surface required about 100 times more AmF than in solution. Our results show that AmF retains its anticariogenic effects in solution and in biofilm systems.

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“…[19,20] It appears to target dextrans in the extracellular matrix by blocking synthesis, [21] reducing viscosity [22] and also by selectively inhibiting dextrans-producing streptococci. [23] Aggregation and Coaggregation:-The interbacterial aggregation include protein-carbohydrate interactions such as the 38-kDa surface protein of S. sanguis with adhesin properties capable of mediating coaggregation with A. naeslundii.…”

Section: Strategies For Biofilm Dispersal:-mentioning

confidence: 99%