Abstract:Background. Ovarian tissue cryopreservation (OTC) is important for fertility preservation and conservation. Delay in OTC can occur for transport or workflow management, yet little is understood about the effect of this on the tissue. Objective. To determine if a delay of 24-48 h to OTC affects primordial follicle (PF) health. Methods. Ovaries (n = 6 sheep) were processed immediately or after storage at 4°C (24 h, 48 h). Tissue was fixed fresh, after cryopreservation or 10-day xenotransplantation. Morphological… Show more
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