The effect of defatted black soldier fly meal (Hermetia illucens) inclusion in the formulated diet on the growth, gene expression, and histopathology of juvenile lobster (Panulirus ornatus Fabricius, 1798)
Abstract:The present study investigated the potential use of defatted black soldier fly (Hermetia illucens) meal as a protein source in the formulated feed for juvenile lobsters, Panulirus ornatus. A total of 160 heads of juvenile lobsters with initial weight of 0.24 ± 0.01 g and initial length of 21.77 ± 0.32 mm were distributed evenly into 16 rectangular fiberglass aquaria and kept individually for 8 weeks of the feeding experiment. Four formulated feeds containing defatted BSF meal as fishmeal replacements at 0%, 25… Show more
“…Copper and zinc were the minerals that have a noticeable difference compared to others. Saputra and Fotedar (2023). *% differences represent the magnitude of amino acids concentration differences between the ingredients and was calculated as (AADFBSFL−AAFFBSFL) / AAFFBSFL *100, where AAi is the amino acids concentration in ingredient i as described by Cummins et al (2017)…”
Abstract. Saputra I, Lee YN. 2023. Nutrition composition of commercial full-fat and defatted black soldier fly larvae meal (Hermetia illucens) as a potential protein resource for aquafeeds. Biodiversitas 24: 4877-4884. Black Soldier Fly or BSF (Hermetia illucens) is one of the most researched insect species for its potential as a protein source in aquafeeds. In the present study, two commercially available full-fat and defatted BSF larvae were analyzed in terms of nutrition profiles including proximate composition, amino acids, fatty acids, and mineral contents. The defatted BSF was in the form of a meal and the full-fat BSF was in the form of overdried. The full-fat BSF larvae were ground using a food-grade milling machine and strained to obtain small particles. BSFL meals were then sent for laboratory analysis. Results indicated that the defatted BSFL have higher crude protein content than full-fat BSF, with crude protein value of 47.70% and 30.72%, respectively. In contrast, the crude lipid in the full-fat BSFL was 36.20%, higher than in defatted BSFL, with only 8.11% lipid content. The amino acids analysis indicated that only histidine was the amino acid that was higher in defatted BSF than in full-fat BSF. The availability of fatty acids in defatted BSFL was lower than in full-fat BSF. On the contrary, the Ca, Na, mg, Zn, Fe, Mn, and Cu in defatted BSF were higher than in the full-fat BSFL. In conclusion, full-fat and defatted BSF have comparable nutrition value to fishmeal sourced from bycatch fishes and the defatting process of BSFL improves the nutritional composition of BSFL including protein and amino acids. Therefore, it can be used as information for the correct choice of BSFL used in aquafeeds.
“…Copper and zinc were the minerals that have a noticeable difference compared to others. Saputra and Fotedar (2023). *% differences represent the magnitude of amino acids concentration differences between the ingredients and was calculated as (AADFBSFL−AAFFBSFL) / AAFFBSFL *100, where AAi is the amino acids concentration in ingredient i as described by Cummins et al (2017)…”
Abstract. Saputra I, Lee YN. 2023. Nutrition composition of commercial full-fat and defatted black soldier fly larvae meal (Hermetia illucens) as a potential protein resource for aquafeeds. Biodiversitas 24: 4877-4884. Black Soldier Fly or BSF (Hermetia illucens) is one of the most researched insect species for its potential as a protein source in aquafeeds. In the present study, two commercially available full-fat and defatted BSF larvae were analyzed in terms of nutrition profiles including proximate composition, amino acids, fatty acids, and mineral contents. The defatted BSF was in the form of a meal and the full-fat BSF was in the form of overdried. The full-fat BSF larvae were ground using a food-grade milling machine and strained to obtain small particles. BSFL meals were then sent for laboratory analysis. Results indicated that the defatted BSFL have higher crude protein content than full-fat BSF, with crude protein value of 47.70% and 30.72%, respectively. In contrast, the crude lipid in the full-fat BSFL was 36.20%, higher than in defatted BSFL, with only 8.11% lipid content. The amino acids analysis indicated that only histidine was the amino acid that was higher in defatted BSF than in full-fat BSF. The availability of fatty acids in defatted BSFL was lower than in full-fat BSF. On the contrary, the Ca, Na, mg, Zn, Fe, Mn, and Cu in defatted BSF were higher than in the full-fat BSFL. In conclusion, full-fat and defatted BSF have comparable nutrition value to fishmeal sourced from bycatch fishes and the defatting process of BSFL improves the nutritional composition of BSFL including protein and amino acids. Therefore, it can be used as information for the correct choice of BSFL used in aquafeeds.
The present study aims to evaluate the effect of liquid fish protein hydrolysate (FPH) following fishmeal substitution with full‐fat and defatted BSF (black soldier fly, Hermetia illucens) meal in the feeds of juvenile ornate spiny lobster, Panulirus ornatus. The physiological aspects of juvenile lobsters including growth, fatty acids profile, and histopathology were observed. Six isoenergetic experimental feeds having a protein‐to‐energy ratio of 26 CP mg kJ−1 were formulated with the substitution of fishmeal at 25% using liquid FPH, full‐fat BSF (FBSF), defatted BSF (DBSF), and their combination. The specific growth rate, final body weight, final total length, and length increment of juvenile lobsters (initial weight was 0.21 ± 0.01 g and total length was 20.53 ± 0.12 mm) were significantly affected by the fishmeal substitution (P < 0.05) and improved with the addition of liquid FPH in the feeds containing FBSF and DBSF. The whole body proximate analysis showed that the liquid FPH to the feeds containing DBSF increased the ash and protein content significantly (P < 0.05). The total monounsaturated fatty acids (∑MUFA), saturated fatty acids (∑SFA), and omega 9 fatty acids (∑n−9 FA) of juvenile lobsters’ whole bodies fed with dietary DBSF and FPH supplementation were significantly higher than those of others (P < 0.05). The histopathological analysis indicated that the villus size and the muscle thickness in the intestine were not significantly affected by FPH supplementation. However, the hepatopancreas histopathology indicated the presence of B‐cells and R‐cells in the juvenile lobsters fed with FPH‐supplemented feeds. The present results suggested the supplementation of liquid FPH to the formulated feed with FBSF and DBSF for juvenile lobsters can improve the lobsters’ growth and fatty acids availability.
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