The effect of dapsone in testosterone enanthate-induced polycystic ovary syndrome in rat

Shirooie, Samira; Khaledi, Ehsan; Dehpour, Ahmad Reza; Noori, Tayebeh; Khazaei, Mozafar; Sadeghi, Faezeh; Sobarzo‐Sánchez, Eduardo

doi:10.1016/j.jsbmb.2021.105977

Cited by 8 publications

(5 citation statements)

References 21 publications

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“…Results of a previous study from our laboratory that had evaluated age-related effects of testosterone administration in gonadally-intact males revealed that the subcutaneous injection of testosterone was associated with weight gain . In this study also the administration of testosterone enantate to weaned female rats was associated with weight gain and metabolic derangement (data not included) which are consistent with PCOS as previously described by Shirooie et al (2021). In the healthy female rats administered clomiphene and/or letrozole a gradual increase in body weight was observed while the co-administration of clomiphene and letrozole was associated with lesser weight gain compared to clomiphene or letrozole alone.…”

Section: Discussionsupporting

confidence: 88%

“…Animals were grouped (Table 2.1) as normal control administered vehicle (normal saline) orally at 10 ml/kg, clomiphene (CLOM at 100 microgram/kg body weight), letrozole (LETR at 5mg/kg body weight) or combined clomiphene and letrozole groups. There were also four groups [Testosterone (TEST) control, TEST/CLOM, TEST/LETR or TEST/CLOM+LETR] of animals in which hyperandrogenism was induced with testosterone enantate administered subcutaneously at 1 mg/100 g body weight (Shirooie et al, 2021). Subcutaneous injection of testosterone or normal saline was administered daily from day 1-35, beginning on day 36, clomiphene, letrozole or saline was administered daily for 10 days.…”

Section: Experimental Methodologymentioning

confidence: 99%

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Induced-hyperandrogenism in female rats: assessing possible modulation of neurobehavioural and neurotransmitter changes following clomiphene/letrozole intervention

Aworinde

Olofinnade

Onaolapo

2023

Preprint

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Hyperandrogenism is the excessive production of androgenic hormones resulting in infertility in a number of women. While letrozole and clomiphene citrate have been used to increase chances of achieving pregnancy, their effects on the brain has been scarcely studied. This study examined the effects of clomiphene and letrozole alone or in combination on neurobehavioural and neurochemical changes in female rats exposed to testosterone. Weaned rats were assigned into eight groups of ten each. Animals were grouped as normal control administered vehicle (normal saline) orally at 10 ml/kg or subcutaneously at 2 ml/kg, three groups administered clomiphene (CLOM) at 100 microgramme/kg, letrozole (LETR) at 5 mg/kg and or a combination of clomiphene and letrozole (CLOM/LETR) orally and saline subcutaneously. There were also four groups Testosterone (Test), Test/CLOM, Test/LETR or Test/CLOM+LETR administered testosterone enantate subcutaneously at 1 mg/100 g. Testosterone or saline was administered from day 1-35, while beginning on day 36, clomiphene, letrozole or saline was administered daily for 10 days. At the end of the dosing period, animals were exposed to different behavioural paradigms. After the behavioural tests, animals were sacrificed, the cerebral cortex was homogenised for the assessment of biochemical assays. The result showed an increase in body weight, food intake, locomotor activity, rearing and self grooming with CLOM, LETR and CLOM/LETR in all treated groups. Decreased spatial working memory and anxiolysis was observed with letrozole and/or clomiphene. Increased oxidative stress, decreased total antioxidant capacity, altered inflammatory cytokines and brain neurotransmitter were observed with letrozole and /or clomiphene. In conclusion, the administration of clomiphene and/or letrozole was associated with significant alterations in brain function, oxidative stress, inflammatory markers and brain neurotransmitter levels.

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Section: Discussionsupporting

confidence: 88%

Section: Experimental Methodologymentioning

confidence: 99%

Induced-hyperandrogenism in female rats: assessing possible modulation of neurobehavioural and neurotransmitter changes following clomiphene/letrozole intervention

Aworinde

Olofinnade

Onaolapo

2023

Preprint

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“…(17) All 4 studies reported to have high risk of bias where it had low quality of studies. The study (59,62,64,66) showed high risk of bias in random sequence generation (selection bias) because no randomization of animal group was done, allocation concealment (selection bias) was high risk as the investigators can foresee the animal groups allocated for intervention, blinding of personnel/ care providers (performance bias) was also high as the animals were not housed randomly, blinding of outcome assessor (detection bias) was also high as the animals were not selected at random for outcome assessment.…”

Section: Risk Of Bias (Rob) Assessmentmentioning

confidence: 99%

The Efficacy of Metformin and Exenatide in Polycystic Ovary Syndrome (PCOS) Patients

Kesavan¹,

Chaudhry²,

Akim³

et al. 2023

Ars Pharm

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Introduction: Polycystic Ovary Syndrome (PCOS) is a hormonal disorder that affects 5-10% of women who are their reproductive age. This meta-analysis aims to evaluate the efficacy of metformin and exenatide, respectively, and to compare the efficacy of both drugs using Body Mass Index (BMI), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and testosterone level. Method: Scopus, Science Direct, Oxford Journal, Wiley Online Library, and Medline (through the PubMed search engine) were used in this study. Statistical analysis of the included studies was done using the RevMan 5.4 software. Results: There were 6 studies included in the analysis of the study. There was a significant reduction in BMI of PCOS patients with exenatide versus metformin (mean difference = 0.51; 95% confidence interval (CI)= 0.07, 0.96, I 2= 52%; p=0.02). There was also a significant reduction in the testosterone level of PCOS patients with exenatide versus metformin (mean difference = 0.15; 95% confidence interval (CI)= 0.07, 0.22, I 2= 0%; p=0.0002). There was no effect on the mean of LDL-C and of HDL-C when compared between metformin and exenatide This meta-analysis shows that exenatide is effective in reducing BMI and testosterone levels in PCOS patients. Conclusions: There were a significant reduction in BMI and testosterone levels of PCOS patients when exenatide was used as compared to metformin. However, there was no effect on the mean of the LDL-C and HDL-C levels of the PCOS patients.

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“…The stained slides were assessed under a light microscope (Olympus BX51, at X4 magnification) equipped with camera, by two blind students. Finally, the mean of antral and primary follicles was evaluated (8,35,36).…”

Section: Biochemical Hormonal and Histological Evaluationmentioning

confidence: 99%

“…PCOS causes histopathological morphology changes in the ovary and also affects the lipid profile and blood glucose levels (8). Obesity which is common in PCOS patients may be the reason for insulin resistance and dyslipidemia (9).…”

Section: Introductionmentioning

confidence: 99%

The Effects of Coenzyme Q10 on Hormonal and Histopathological Changes in Female Wistar Rats with Polycystic Ovary Syndrome

Pak-Hashemi

Taghipour

Fatemi

et al. 2023

Journal of Kerman University of Medical Sciences

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Background: Polycystic ovary syndrome (PCOS) is one the most common diseases in women in the fertility age. PCOS alters ovarian follicles and affects blood lipid profiles, liver enzymes and hormones. In this study, we tried to investigate the effects of coenzyme Q10 (CoQ10) on PCOS pathological conditions. Methods: Twenty-four female rats were randomly divided into the three groups; 1: control group, 2: PCOS group (received 1 mg/ kg letrozole daily for 21 days, PO) and 3: PCOS+CoQ10 group (received 1 mg/kg letrozole with 10 mg/kg of CoQ10 for 21 days orally). Blood lipid profiles, liver enzymes, blood glucose and testosterone levels were measured and ovarian histopathology was evaluated. Results: Histological examination showed reduced number of antral follicles in PCOS+CoQ10 group as compared with PCOS group (P<0.001). PCOS increased the alanine aminotransferase (ALT) enzyme by acting on the liver (P<0.01). Administration of CoQ10 during PCOS induction was able to reduce ALT levels (P<0.01). PCOS increased the cholesterol (P<0.05), fasting blood sugar (FBS) (P<0.05), triglyceride (P<0.001), low-density lipoprotein (LDL) (P<0.05) and testosterone levels (P<0.001) compared to the control group. However, CoQ10 treatment significantly reduced the above-mentioned parameters compared to the PCOS group. Conclusion: The results of the present study confirm that CoQ10 has a therapeutic potential for PCOS-induced lipid and hormonal changes and ovarian tissue damages. CoQ10 supplementation and its concomitant use with letrozole could inhibit the development of PCOS in rats. Testosterone reduction could be an important mechanism for CoQ10 beneficial effects.

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The effect of dapsone in testosterone enanthate-induced polycystic ovary syndrome in rat

Cited by 8 publications

References 21 publications

Induced-hyperandrogenism in female rats: assessing possible modulation of neurobehavioural and neurotransmitter changes following clomiphene/letrozole intervention

Induced-hyperandrogenism in female rats: assessing possible modulation of neurobehavioural and neurotransmitter changes following clomiphene/letrozole intervention

The Efficacy of Metformin and Exenatide in Polycystic Ovary Syndrome (PCOS) Patients

The Effects of Coenzyme Q10 on Hormonal and Histopathological Changes in Female Wistar Rats with Polycystic Ovary Syndrome

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