The Effect of d-Aspartate on Spermatogenesis in Mouse Testis1

Tomita, Kengo; Tanaka, Hiroyuki; Kageyama, Susumu; Nagasawa, Mitsuru; Wada, Akinori; Murai, Ryosuke; Kobayashi, Kenichi; Hanada, Eiki; Agata, Yasutoshi; Kawauchi, Akihiro

doi:10.1095/biolreprod.115.134692

Cited by 19 publications

(19 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although all DA treatments resulted in significant improvement in fertility, motility and plasma membrane integrity compared with control, the moderate level of treatment (DA200) was significantly better when compared with the other DA treatments. The diminished return from increasing the dose of DA can be explained by the fact that the DA concentration is controlled by DA oxidase in Sertoli cells, which catalyses DA degradation to oxaloacetate, ammonium and hydrogen peroxide (Tomita et al 2016). The resulting increase in hydrogen peroxide production in the group treated with the higher dose (300 mg kg À1 day À1 ) in the present study may have attenuated some of the positive effects of DA on the investigated traits.…”

Section: Discussionmentioning

confidence: 56%

D-Aspartate amends reproductive performance of aged roosters by changing gene expression and testicular histology

Ansari

Zhandi

Kohram

et al. 2018

Reprod. Fertil. Dev.

View full text Add to dashboard Cite

Male broiler breeders (n = 32) of 55 weeks of age were administered four different doses of capsulated d-aspartate (DA; 0, 100, 200 or 300 mg kg−1 day−1, p.o. (DA0, DA100, DA200 and DA300 respectively)) for 12 successive weeks to assess reproductive performance, blood testosterone, testicular histology and transcript levels of steroidogenic acute regulatory protein (StAR), cholesterol side-chain cleavage enzyme (P450scc), androgen receptor (AR), LH receptor (LHR), 3β-hydroxysteroid dehydrogenase (3BHSD), proliferating cell nuclear antigen (PCNA), glutamate ionotropic receptor NMDA type subunit 1 (GRIN1) and glutamate ionotropic receptor NMDA type subunit 2B (GRIN2B). Blood samples and ejaculates were collected, and bodyweight was recorded weekly for 10 weeks. AI was performed weekly for the last 2 weeks to determine the number of sperm penetration holes in the perivitelline layer, fertility and hatchability. Testes histology and transcript levels were evaluated in the 12th week. Bodyweight, numbers of Leydig cells and blood vessels, testis index and levels of sperm abnormalities were not affected (P > 0.05) by the treatment. However, sperm total and forward motility, plasma membrane integrity and functionality of sperm, ejaculate volume, testosterone concentration and fertility were higher (P < 0.05) in both the DA200 and DA300 groups compared with the other groups. In the DA100 and DA200 groups, sperm concentration, number of spermatogonia, thickness of the seminiferous epithelium and the diameter of tubules were significantly higher (P < 0.05) than the other DA-treated groups. The number of penetration holes, hatchability and malondialdehyde concentration were higher in the DA200, all DA-treated and DA300 groups respectively compared with the control and other treatment groups. Except for P450scc, AR, LHR and PCNA transcript levels in the DA300 groups, the relative expression of the genes evaluated improved significantly in the other DA-treated groups. Based on these experimental findings, it is concluded that DA improves reproductive performance of aged roosters.

show abstract

Section: Discussionmentioning

confidence: 56%

D-Aspartate amends reproductive performance of aged roosters by changing gene expression and testicular histology

Ansari

Zhandi

Kohram

et al. 2018

Reprod. Fertil. Dev.

View full text Add to dashboard Cite

show abstract

“…It is well known that stem cell factor/Kitlg (more commonly known as c-kit) signal induces spermatogonial proliferation. In contrast, Tomita and co-workers [ 54 ] found a negative effect of d -Asp on the differentiation of premeiotic germ cells.…”

Section: D -Asp and Spermatogonial Proliferatiomentioning

confidence: 88%

“…Recently, Tomita and coworkers [ 54 ] have demonstrated that in mouse endogenous d -Asp preferentially accumulates in differentiated spermatids, indicating either transport of d -Asp from other cell type(s) or de novo synthesis in these cells.…”

Section: D -Asp and Spermatozoa Maturationmentioning

confidence: 99%

Molecular Mechanisms Elicited by d-Aspartate in Leydig Cells and Spermatogonia

Fiore¹,

Santillo²,

Falvo³

et al. 2016

IJMS

View full text Add to dashboard Cite

A bulk of evidence suggests that d-aspartate (d-Asp) regulates steroidogenesis and spermatogenesis in vertebrate testes. This review article focuses on intracellular signaling mechanisms elicited by d-Asp possibly via binding to the N-methyl-d-aspartate receptor (NMDAR) in both Leydig cells, and spermatogonia. In Leydig cells, the amino acid upregulates androgen production by eliciting the adenylate cyclase-cAMP and/or mitogen-activated protein kinase (MAPK) pathways. d-Asp treatment enhances gene and protein expression of enzymes involved in the steroidogenic cascade. d-Asp also directly affects spermatogonial mitotic activity. In spermatogonial GC-1 cells, d-Asp induces phosphorylation of MAPK and AKT serine-threonine kinase proteins, and stimulates expression of proliferating cell nuclear antigen (PCNA) and aurora kinase B (AURKB). Further stimulation of spermatogonial GC-1 cell proliferation might come from estradiol/estrogen receptor β (ESR2) interaction. d-Asp modulates androgen and estrogen levels as well as the expression of their receptors in the rat epididymis by acting on mRNA levels of Srd5a1 and Cyp19a1 enzymes, hence suggesting involvement in spermatozoa maturation.

show abstract

“…The increase of L-Asp could be explained by the increase in oxaloacetate level which, via glutammic-oxaloacetic transaminase [ 35 ], can be converted into L-Asp in an almost equilibrium reaction. On the other hand, D-Asp levels were shown to increase in mice testis during development [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Age-dependent changes in metabolic profile of turkey spermatozoa as assessed by NMR analysis

et al. 2018

View full text Add to dashboard Cite

Metabolic profile of fresh turkey spermatozoa at three different reproductive period ages, namely 32, 44 and 56 weeks, was monitored by Nuclear Magnetic Resonance (NMR) spectroscopy and correlated to sperm quality parameters. The age-related decrease in sperm quality as indicated by reduction of sperm concentration, sperm mobility and osmotic tolerance was associated to variation in the level of specific water-soluble and liposoluble metabolites. In particular, the highest levels of isoleucine, phenylalanine, leucine, tyrosine and valine were found at 32 weeks of age, whereas aspartate, lactate, creatine, carnitine, acetylcarnitine levels increased during the ageing. Lipid composition also changed during the ageing: diunsaturated fatty acids level increased from 32 to 56 weeks of age, whereas a reduction of polyunsaturated fatty acids content was observed at 56 weeks. The untargeted approach attempts to give a wider picture of metabolic changes occurring in ageing suggesting that the reduction of sperm quality could be due to a progressive deficiency in mitochondrial energy producing systems, as also prompted by the negative correlation found between sperm mobility and the increase in certain mitochondrial metabolites.

show abstract

The Effect of d-Aspartate on Spermatogenesis in Mouse Testis1

Cited by 19 publications

References 22 publications

D-Aspartate amends reproductive performance of aged roosters by changing gene expression and testicular histology

D-Aspartate amends reproductive performance of aged roosters by changing gene expression and testicular histology

Molecular Mechanisms Elicited by d-Aspartate in Leydig Cells and Spermatogonia

Age-dependent changes in metabolic profile of turkey spermatozoa as assessed by NMR analysis

Contact Info

Product

Resources

About