The Effect of Cyclosporin A on Airway Cell Proinflammatory Signaling and Pneumonia

Abstract

(Received in original form January 5, 2005 and in final form April 11, 2005) This work was supported by an NIH grant (HL60293) to A.P. and by a Cystic Fibrosis Foundation Fellowship to V.W.

Mycobacterium bovisBacillus Calmette-Guérin Induces CCL5 Secretion via the Toll-Like Receptor 2-NF-κB and -Jun N-Terminal Kinase Signaling Pathways

“…Our data demonstrate that calcineurin plays an important role in the mediation of TLR2-stimulated CCL5 production by M. bovis BCG-infected cells, since we found that TLR2-mediated induction of CCL5 was greatly reduced by the calcineurin inhibitor FK-506. This finding is in excellent agreement with previous studies showing that another calcineurin inhibitor, cyclosporine, achieved significant inhibition of TLR2-mediated induction of CXCL8 secretion (38). It is interesting to consider the potential effects of calcineurin on several different transcription factors that could be involved in CCL5 production.…”

Calcineurin activates interleukin-6 transcription in mouse skeletal muscle in vivo and in C2C12myotubes in vitro

“…Finally, we chose to examine the role of two other transcription factor families, CREB and C/EBP, in basal and calcineurin-activated IL-6 promoter activity for three reasons: 1) binding sites for these transcription factors have been previously identified in the IL-6 promoter region, and they are known to regulate IL-6 promoter activity in other cell types (13,18,37,54); 2) their activity can be altered by calcineurin signaling (7,28,31,56,64); 3) repression of MEF-2 activity by MITR cotransfection was unable to eliminate all of the calcineurin induction of the IL-6 promoter, suggesting that other pathways contribute to this response. We found that, as has been shown previously for other cell types (13,18,37,54), mutation of the CREB or C/EBP binding sites alone significantly attenuated basal IL-6 promoter activity, and mutation of both together decreased basal IL-6 promoter activity to ϳ10% of wild type.…”

“…Finally, because CREB and the C/EBP family of transcription factors can also be activated by calcineurin signaling (7,31,57,64) and are known to regulate IL-6 transcription in other cell types (13, 18, 37, 54), we evaluated the effects of these transcription factors on basal and calcineurin-stimulated IL-6 promoter activity. Cotransfection with either a constitutively active CREB or a wild-type C/EBP-␦ construct was sufficient to significantly increase IL-6 promoter activity in C 2 C 12 myotubes (Fig.…”

Effect of Alcoholic Extract of Salvia officinalis Leaves on some Physiological Parameters Aspects in Acrylamide-Treated Rats

“…Patients with immune suppression therapy have been shown to have elevated level in the serum IL-6 (Tilg et al, 1992). The pro-inflammatory effect of Cys (Waters et al, 2005) and its effect on expression of IL-2 drives IL-6 secretion (Musso et al, 1992) was documented. In contrast to the current result long term treatment (more than one year) with cyclosporine caused significant decrease in serum IL-6 (Hanudle et al, 2016) and CRP (Madhok et al, 1991).…”