The effect of CVVHD and endotoxin  on the oxidative burst, adhesion molecules and distribution in tissues of granulocytes

Toft, Palle; Krog, Jan; Christensen, Vibeke Brix; Beck, James M.; Dagnæs‐Hansen, Frederik; Obel, Niels; Bendix-Hansen, Knud; Stødkilde-Jørgensen, Hans

doi:10.1007/s001340051245

Cited by 5 publications

(15 citation statements)

References 20 publications

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“…The observed granulocytopenia following endotoxin infusion has been described in other investigations [10] . In the present study, plasmapheresis had no effect on the number of the neutrophils in peripheral blood.…”

Section: Discussionmentioning

confidence: 61%

“…Most studies in septic patients have reported an increased expression of CD18 and a decreased expression of CD62L on neutrophils in septic patients, and following infusion of endotoxin [10,13,14] . This is in accordance with the present study.…”

Section: Discussionmentioning

confidence: 99%

“…The following antibodies were used: anti-CD18-FITC, anti-CD44-FITC, and anti-CD62L-FITC (BD Biosciences, Brøndby, Denmark). The method has been described in detail elsewhere [10] .…”

Section: Adhesion/activation Molecules On Neutrophilsmentioning

confidence: 99%

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Effect of Plasmapheresis on the Immune System in Endotoxin-Induced Sepsis

Toft

Schmidt²,

Broechner³

et al. 2008

Blood Purif

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Background: It has been proposed that plasmapheresis is most effective when applied early in Gram-negative sepsis. We therefore studied the effect of early plasmapheresis on immunity in experimental Escherichia coli endotoxin-induced sepsis. Methods: 20 pigs received 30 µg/kg of E. coli endotoxin. 40 min later, half of the pigs were treated with plasmapheresis which lasted 4 h. The adhesion molecules, the oxidative burst, the number of neutrophils in blood and lungs, and cytokines were measured. Results: Infusion of endotoxin was associated with activation of adhesion molecules increased oxidative burst, increased concentration of cytokine, and accumulation of granulocytes in lung tissue. Plasmapheresis reduced the oxidative burst, and there was a tendency towards a reduced accumulation of granulocytes in the lung. Conclusion: Though plasmapheresis was initiated early after the endotoxin infusion, it only temporarily attenuated a part of the activated cell-mediated immunity.

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Section: Discussionmentioning

confidence: 61%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Plasmapheresis on the Immune System in Endotoxin-Induced Sepsis

Toft

Schmidt²,

Broechner³

et al. 2008

Blood Purif

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show abstract

“…In the literature, the cellular receptors, inflammatory mediators, and cellular functions as described in the present manuscript were used as relevant biomarkers to test the biocompatibility of catheters and vascular grafts, e.g., in cardiopulmonary surgery and dialysis [7][8][9]. However, until today, there are no data about the biocompatibility of silvercoated polyester vascular grafts available.…”

Section: Discussionmentioning

confidence: 98%

“…Thus, activation of selectins and integrins can be used as a biomarker for interaction with the vascular graft [3,7,9,[11][12][13][14]. In particular, shedding of the Lselectin (CD62L) followed by increased expression of the β 2 integrin CD11b/CD18 at the neutrophil surface is one of the main mechanisms underlying transendothelial cellular migration.…”

Section: Discussionmentioning

confidence: 99%