The effect of culture media on the number and bioactivity of marine invertebrates associated fungi

Abstract

Abstract. Trianto A, Radjasa OK, Sibero MT, Sabdono A, Haryanti D, Zilullah WOM, Syanindyta AR, Bahry MS, Widiananto PA, Helmi M, Armono HD, Supriadi, Igarashi Y. 2020. The effect of culture media on the number and bioactivity of marine invertebrates associated fungi. Biodiversitas 21: 407-412. Marine ecosystem is rich with microorganisms such as bacteria and fungi either as free-living or in association with macro-organisms. Marine invertebrates provide suitable habitats for fungi by supplying space, food, and other chemicals stuff that in some cases is a reciprocal relationship or called mutualism symbiotic. Some marine invertebrates have interesting activities that are useful for human life such as anticancer, antifungal, and antibacterial. Many reports indicated that the fungal growth and their production of bioactive compounds were highly affected by the media or nutrition. In order to understand the effect of media on the number and bioactivity of the isolates, we collected the samples of marine invertebrates from two locations in Makassar. Invertebrate specimens were collected by hand during SCUBA diving at 3-10 m depths. The fungi were isolated by tapping method either on potato dextrose agar (PDA) or poor marine agar (PMA). The samples were collected from the Samalona water as much as 16 specimens that provided 30 and 18 fungal isolates on PDA and PMA, respectively, while, from the Barrang Cadi water, a total 14 specimens were collected to provide 12 and 3 isolates on PDA and PMA, respectively. All fungi from PMA inhibited the V. harveyi, V. vulnificus, and V. parahaemolyticus with weak, medium, and strong activities, while, the isolates from PDA were mostly not active against the Vibrios. Based on the molecular analyses, the active isolates were identified as Aspergillus flavus, A. oryzae, A. aculeatus, Talaromyces minioluteus, Hypocrea jecorina, Gliomastix murorum, Myrothecium inundatum, and Curvularia avinis. In conclusion, the isolates from PMA showed higher potential as source of antivibrio substances.

Bioprospecting of cow's ruminal microbiota from a slaughterhouse in Ambarawa, Central Java, Indonesia

“…and other actinobacteria, while PDA for fungi; however, other taxa may grow during the incubation (Kharel et al 2010;Nero et al 2006;Sibero et al 2018). In addition, the nutrient rich media was observed to gain more microorganisms rather than the poor nutrient media (Trianto et al 2020). MRSA plates were incubated in vacuumed conditions during the isolation treatment, while ISP4 and PDA plates were without vacuum.…”

Antibacterial activity of n-hexane and ethanol extracts of Polycarpa aurata against pathogenic bacteria of shrimp and fish

“…The extract had higher antibacterial activity against V. harveyi than V. parahaemolyticus. Antibacterial substances are excreted in an inhibitory zone seen in the test, which indicates that the metabolites of P. aurata extract can kill bacteria around these substances [21].…”

Potential of marine sponge-derived fungi in the aquaculture system

“…Fungal isolation was performed by tapping method according to Trianto et al (2020). Sponge samples were cleaned using sterile marine water to remove microbial contaminant on the surface of the sponge then cut into approximately 1x1cm and tapped into the sterile PDA plate (Merck, Germany) with three repetitions.…”

“…The anti-vibrio assay was conducted by the agar plug method (Sabdaningsih et al 2017;Trianto et al 2020). A total of 3 vibriosis causative (Vibrio harveyi, V. parahaemolyticus, and V. vulnificus), collection of Tropical Marine Biotechnology Undip Laboratory, Semarang were used for anti-vibrio screening.…”