The Effect of Cucurbit[7]uril on the Antitumor and Immunomodulating Properties of Oxaliplatin and Carboplatin

Abstract

Cucurbit[7]uril (CB[7]) is a molecular container that may form host–guest complexes with platinum(II) anticancer drugs and modulate their efficacy and safety. In this paper, we report our studies of the effect of CB[7]–oxaliplatin complex and the mixture of CB[7] and carboplatin (1:1) on viability and proliferation of a primary cell culture (peripheral blood mononuclear cells), two tumor cell lines (B16 and K562) and their activity in the animal model of melanoma. At the same time, we studied the impact of platinum (II) drugs with CB[7] on T cells and B cells in vitro. Although the stable CB[7]–carboplatin complex was not formed, the presence of cucurbit[7]uril affected the biological properties of carboplatin. In vivo, CB[7] increased the antitumor effect of carboplatin, but, at the same time, increased its acute toxicity. Compared to free oxaliplatin, its complex with CB[7] shows a greater cytotoxic effect on tumor cell lines B16 and K562, while in vivo, the effects of the free drug and encapsulated drug were comparable. However, in vivo studies also demonstrated that the encapsulation of oxaliplatin in CB[7] lowered the toxicity of the drug.

Modulation of the Fibrillation Kinetics and Morphology of a Therapeutic Peptide by Cucurbit[7]uril

“…On the basis of these observations, we propose to further characterize different fibril morphologies obtained via the addition of CB[7] for ENF and, potentially, other peptides. This would be of particular interest since different fibril morphologies have been reported to exhibit distinct physicochemical properties and stabilities that have been exploited for drug delivery and biotechnological applications. , For therapeutic applications, we are not aware of detailed toxicological studies on CB[7], although we note that it has been used as an excipient for anticancer agents in animal models, without evidence of direct toxic effects . Further, its use in reducing the toxicity of paraquat and neurotoxic drugs has been reported, suggesting potential for in vivo compatibility.…”

Psoralidin–cucurbit[7]uril complex with improved solubility to tackle human colorectal cancer: experimental and computational study

“…For instance, CB7 improved the antitumor activity of carboplatin and oxaliplatin, platinumbased anticancer drugs. 45 The anticancer activity of Ps against HT-29 and HCT-116 colon cancer cells has been previously established. 46 Several mechanisms have been suggested.…”

Alginate supramolecular hydrogels based on viologen and cucurbit[8]uril: Host‐induced caveolae‐mediated endocytosis to white blood cancer cells

“…The role of CB8 in triggering cellular internalization to white blood cancer cells was investigated. While it is not uncommon to see such systems at work in small organic molecules that change their cellular uptake under the influence of CB macrocycles, 22 using such systems for extended networked structures such as the present CB8‐based alginate hydrogels (VGCB8ALG) is purely academic and should unfold more information.…”