The effect of Cu2+ on interaction between flavonoids with different C-ring substituents and bovine serum albumin: Structure–affinity relationship aspect

Zhang, Yuping; Shi, Shuyun; Sun, Xiaorui; Xiong, Xiang; Peng, Mijun

doi:10.1016/j.jinorgbio.2011.08.007

Cited by 40 publications

(31 citation statements)

References 47 publications

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“…They have reported a qualitatively similar hypsochromic shift with respect to BSA. Zhang et al, 2011 13 have also reported a similar qualitative blue shift with respect to quercetin (0-10 µM) only. Shifts pertaining to luteolin were not shown.…”

Section: Discussionmentioning

confidence: 73%

“…12 Other reasons could be alterations in the associations of the subunits, denaturation of the protein or may be dependent on substrate binding-mediated events. 13 To the best of our knowledge, there are no reports wherein a systematic comparison has been made with respect to the reproducible, luteolin versus quercetinmediated UV-visible hypsochromic shifts, using a particular range of concentrations (3.33 -33 µM of luteolin versus quercetin) with a constant concentration of BSA (1.51 × 10 -5 M = 15.1 µM). Further, our report has specifically documented a hitherto unreported UV-visible spectroscopy-based 3 and 4.1 nm shifts for luteolin and quercetin respectively.…”

Section: Discussionmentioning

confidence: 99%

“…16 Fluorescence spectroscopy results seem to point towards a static quenching mechanism under our defined experimental conditions (Table 1a and 1b). 5,7,8,10,13,14,17 There have been very few reports wherein a comparison has been made between luteolin and quercetin with respect to fluorescence spectroscopy data. 13,17 Unlike the experimental flow in these reports, we have adopted an experimental design wherein thermodynamic and docking data have also been incorporated (please see below).…”

mentioning

confidence: 99%

“…5,7,8,10,13,14,17 There have been very few reports wherein a comparison has been made between luteolin and quercetin with respect to fluorescence spectroscopy data. 13,17 Unlike the experimental flow in these reports, we have adopted an experimental design wherein thermodynamic and docking data have also been incorporated (please see below). Further, the possibility of dynamic quenching also playing a role in this process cannot be ruled out since measurements taken following incubation for a shorter duration may have revealed the relative contribution of dynamic quenching 2,14 in this process.…”

mentioning

confidence: 99%

“…(Table 2a and Table 2b). 13 Despite other papers documenting a number higher than 1, raising the possibility of the existence of more than one binding site, 5,8,14,16 definitive evidence regarding this is lacking. The positive entropy change ΔS 0 is indicative of the presence of hydrophobic interactions.…”

mentioning

confidence: 99%

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A Correlative Multi-Spectroscopy and Docking Study for the Modeling of Drug (Luteolin and Quercetin) Binding to Bovine Serum Albumin– A Tool for the Determination of Binding Characteristics to Receptor Proteins

Krishnan¹,

Hiray²,

Vyas³

2018

IJPER

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The in vitro in silico experimental flow (multi-spectroscopy and docking) demonstrated the binding of Luteolin and Quercetin separately with Bovine Serum Albumin. For the first time, we are reporting the relative UV-visible spectroscopy-based hypsochromic shifts for both luteolin (3nm) and quercetin (4.1 nm) respectively. The drug-induced conformational change may lead to the possible shift in the tryptophan residue to a more hydrophobic environment. Our demonstration of an increased static quenching of the endogenous fluorophore in BSA validated the UV-visible spectroscopy data. However, detailed experiments will further delineate the possible relative contribution of dynamic quenching processes. The strong binding (binding constant values -10 5 L/mol) and the number of binding sites (1 for luteolin and quercetin) is consistent with published findings. Under our defined conditions, the hitherto unreported non-cooperative binding was demonstrated, based on the Hill's coefficient. Thermodynamic data qualitatively validated hydrophobicity (a positive entropy change ΔS 0 ); hydrogen bonding (a negative ΔH 0 ) and electrostatic interactions (a negative ΔH 0 and a positive ΔS 0 ). For the first time, the Infra-Red Spectroscopy (FT-IR) data showed ground state complex formation of the molecules with the model protein and may serve to corroborate our fluorescence (static quenching) data. Hydrogen bonds and hydrophobic interactions for both molecules (Ligplot Analysis) provide corroborative evidence for the molecular spectroscopy and thermodynamic data. This hitherto unreported, unique, combinatorial in vitro (multispectroscopy and thermodynamic measurements) in silico (docking and Ligplot-based analysis) experimental flow (specifically for luteolin and quercetin) provides a basis for extending such binding studies for novel receptors and/or ligands.

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Section: Discussionmentioning

confidence: 73%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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A Correlative Multi-Spectroscopy and Docking Study for the Modeling of Drug (Luteolin and Quercetin) Binding to Bovine Serum Albumin– A Tool for the Determination of Binding Characteristics to Receptor Proteins

Krishnan¹,

Hiray²,

Vyas³

2018

IJPER

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The effects of experimental conditions of fluorescence quenching on the binding parameters of apigenin to bovine serum albumin by response surface methods

2013

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A fluorescence quenching technique is often used to study interactions between small molecules and serum albumin. However, the results are quite different by using spectroscopic techniques on the same drug-protein interaction research and they may be affected by different conditions (e.g. working solution of pH and ionic strength). In this research, using apigenin as an example, the effect of experimental conditions of fluorescence quenching on the binding parameters of drug to bovine serum albumin was investigated using a response surface method (RSM). The effect of pH, the concentration of NaCl and the concentration Mg(2+) on the quenching constant (KSV), the apparent association constant (Ka) and the number of binding sites (n) was studied by single-factor experiments with pH, [NaCl] and [Mg(2+)] as independent variables and KSV, Ka and n as response values. Prediction models were fit to a quadratic polynomial regression equation and the results showed that both KSV and n displayed a second-order model, whereas Ka displayed linear relation dependence on pH, [NaCl] and [Mg(2+)]. Under these experimental conditions, [NaCl] was the most significant (p < 0.05) impact factor on KSV and Ka, whereas n was most affected by pH (p < 0.05).

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Effects of metals released in strong‐flavor baijiu on the evolution of aroma compounds during storage

Huang

Zeng

Liu³

et al. 2020

Food Science & Nutrition

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Storage is essential in improving the quality of strong-flavor baijiu (SFB). Here, we investigated the release behaviors of metals from containers into SFB and their effects on the evolution of aroma compounds during storage. Twenty-six metals were identified in SFB samples. The concentrations of Na, K, Ca, Mg, Al, and Fe obviously increased after storing in pottery jar, whereas those of Fe and Cu greatly increased after storing in stainless-steel vessel. The volatility of most esters, alcohols, ketone, furan, and aldehyde decreased, whereas that of most acids increased after adding the metal ions into fresh SFB. The fluorescence intensity of SFB decreased with increased aging time in pottery jar, whereas the fluorescence intensity of acids was quenched with adding Fe 3+ and Cu 2+ . All these results suggested that some metals released from containers had binding affinities with acids, thereby reducing SFB organoleptic stimulation by forming metal-aroma compound complexes during storage. K E Y W O R D S aroma compounds, metals, storage, strong-flavor baijiu | 1905 HUANG et Al.affect the taste and smell of baijiu. Xiong, Xiang, and Zhao (2013) reported that Ca, Fe, Mg, and Cu released from pottery jar into baijiu improved the soft mellow and harmonious mouthfeel. However, the relationship between metals and flavor compounds of SFB, which is closely related to SFB quality, remains unclear because the flavor compounds are too complex. Metals play an important role in modulating sensory attributes S U PP O RTI N G I N FO R M ATI O NAdditional supporting information may be found online in the Supporting Information section. Effects of metals released in strong-flavor baijiu on the evolution of aroma compounds during storage. Food Sci

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The effect of Cu2+ on interaction between flavonoids with different C-ring substituents and bovine serum albumin: Structure–affinity relationship aspect

Cited by 40 publications

References 47 publications

A Correlative Multi-Spectroscopy and Docking Study for the Modeling of Drug (Luteolin and Quercetin) Binding to Bovine Serum Albumin– A Tool for the Determination of Binding Characteristics to Receptor Proteins

A Correlative Multi-Spectroscopy and Docking Study for the Modeling of Drug (Luteolin and Quercetin) Binding to Bovine Serum Albumin– A Tool for the Determination of Binding Characteristics to Receptor Proteins

The effects of experimental conditions of fluorescence quenching on the binding parameters of apigenin to bovine serum albumin by response surface methods

Effects of metals released in strong‐flavor baijiu on the evolution of aroma compounds during storage

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