The Effect of Cryopreservation on Clonogenic Capacity and In Vitro Expansion Potential of Umbilical Cord Blood Progenitor Cells

Moezzi, Leili; Pourfathollah, Aliakbar; Soleimani, Masoud; Ardjmand, Alireza

doi:10.1016/j.transproceed.2005.10.107

Cited by 16 publications

(13 citation statements)

References 14 publications

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“…Many studies have been performed intended to prevent loss of cell viability after freezing as well as to decrease toxicity during PBPC infusion, by using diverse cryopreservation protocols and/or changing cell or DMSO concentration. Unfortunately, as occurred in our study, cell viability after thawing always decreases 2,4,5,21‐23 . Importantly in our study, cells to be infused cryopreserved in the bags showed similar or even better viability recovery than cells conserved in the cryotube in the NHL patients, Cy+CSF mobilization, and higher cell concentration groups.…”

Section: Discussionsupporting

confidence: 45%

Cryopreservation impact on blood progenitor cells: influence of diagnoses, mobilization treatments, and cell concentration

et al. 2010

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Section: Discussionsupporting

confidence: 45%

Cryopreservation impact on blood progenitor cells: influence of diagnoses, mobilization treatments, and cell concentration

et al. 2010

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“…The use of cryopreserved HSCs would present a convenient option for immunotherapy. However, different studies have reported that expansion of frozen HSC is often poor [17], with a decreased cell count and viability [18], while others have reported that frozen CBCD34 + can be used to generate NK cells [13] because of their high proliferative and clonogenic capacity [19]. In addition, with the rapid advancement in technology and new protocols supporting NK cell generation in vitro , the need to define which source of HSC, CBCD34 + or PBCD34 + , fresh or frozen, is better for this approach is critical.…”

Section: Introductionmentioning

confidence: 99%

Frozen Cord Blood Hematopoietic Stem Cells Differentiate into Higher Numbers of Functional Natural Killer Cells In Vitro than Mobilized Hematopoietic Stem Cells or Freshly Isolated Cord Blood Hematopoietic Stem Cells

Luevano

Domogala²,

Blundell³

et al. 2014

PLoS ONE

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Adoptive natural killer (NK) cell therapy relies on the acquisition of large numbers of NK cells that are cytotoxic but not exhausted. NK cell differentiation from hematopoietic stem cells (HSC) has become an alluring option for NK cell therapy, with umbilical cord blood (UCB) and mobilized peripheral blood (PBCD34+) being the most accessible HSC sources as collection procedures are less invasive. In this study we compared the capacity of frozen or freshly isolated UCB hematopoietic stem cells (CBCD34+) and frozen PBCD34+ to generate NK cells in vitro. By modifying a previously published protocol, we showed that frozen CBCD34+ cultures generated higher NK cell numbers without loss of function compared to fresh CBCD34+ cultures. NK cells generated from CBCD34+ and PBCD34+ expressed low levels of killer-cell immunoglobulin-like receptors but high levels of activating receptors and of the myeloid marker CD33. However, blocking studies showed that CD33 expression did not impact on the functions of the generated cells. CBCD34+-NK cells exhibited increased capacity to secrete IFN-γ and kill K562 in vitro and in vivo as compared to PBCD34+-NK cells. Moreover, K562 killing by the generated NK cells could be further enhanced by IL-12 stimulation. Our data indicate that the use of frozen CBCD34+ for the production of NK cells in vitro results in higher cell numbers than PBCD34+, without jeopardizing their functionality, rendering them suitable for NK cell immunotherapy. The results presented here provide an optimal strategy to generate NK cells in vitro for immunotherapy that exhibit enhanced effector function when compared to alternate sources of HSC.

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“…Recently, hUCB progenitor cells were shown to possess significant advantages over bone marrow in terms of proliferative capacity and immunologic reactivity (26). Therefore, hUCB may be considered as an attractive source of hematopoietic stem cells for both research and clinical applications.…”

Section: Introductionmentioning

confidence: 99%

Umbilical Cord Blood Stem Cell Mediated Downregulation of Fas Improves Functional Recovery of Rats after Spinal Cord Injury

Dasari

Spomar

et al. 2007

Neurochem Res

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Human umbilical cord blood stem cells (hUCB), due to their primitive nature and ability to develop into nonhematopoietic cells of various tissue lineages, represent a potentially useful source for cellbased therapies after spinal cord injury (SCI). To evaluate their therapeutic potential, hUCB were stereotactically transplanted into the injury epicenter, one week after SCI in rats. Our results show the presence of a substantial number of surviving hUCB in the injured spinal cord up to five weeks after transplantation. Three weeks after SCI, apoptotic cells were found especially in the dorsal white matter and gray matter, which are positive for both neuron and oligodendrocyte markers. Expression of Fas on both neurons and oligodendrocytes was efficiently downregulated by hUCB. This ultimately resulted in downregulation of caspase-3 extrinsic pathway proteins involving increased expression of FLIP, XIAP and inhibition of PARP cleavage. In hUCB-treated rats, the PI3K/Akt pathway was also involved in antiapoptotic actions. Further, structural integrity of the cytoskeletal proteins α-tubulin, MAP2A&2B and NF-200 has been preserved in hUCB treatments. The behavioral scores of hind limbs of hUCB-treated rats improved significantly than those of the injured group, showing functional recovery. Taken together, our results indicate that hUCB-mediated downregulation of Fas and caspases leads to functional recovery of hind limbs of rats after SCI.

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The Effect of Cryopreservation on Clonogenic Capacity and In Vitro Expansion Potential of Umbilical Cord Blood Progenitor Cells

Cited by 16 publications

References 14 publications

Cryopreservation impact on blood progenitor cells: influence of diagnoses, mobilization treatments, and cell concentration

Cryopreservation impact on blood progenitor cells: influence of diagnoses, mobilization treatments, and cell concentration

Frozen Cord Blood Hematopoietic Stem Cells Differentiate into Higher Numbers of Functional Natural Killer Cells In Vitro than Mobilized Hematopoietic Stem Cells or Freshly Isolated Cord Blood Hematopoietic Stem Cells

Umbilical Cord Blood Stem Cell Mediated Downregulation of Fas Improves Functional Recovery of Rats after Spinal Cord Injury

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