The effect of crude drugs on the angiogenic property and dynamic viscoelasticity of PEMA-based soft polymer materials

Wang, Weiqi; Hong, Guang; Han, Jian; Murata, Hiroshi; Sasaki, Keiichi

doi:10.4012/dmj.2016-387

Cited by 1 publication

(1 citation statement)

References 31 publications

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“…2 Crude astragalus released from a soft polymer was reported to promote tube formation of the cultured endothelial cells. 3 Radix puerariae (Gegen) is another botanic drug widely used in TCM to treat a variety of symptoms including fever and diarrhea. Studies found that puerarin can improve osteogenesis and angiogenesis 4 and enhance the function of infarcted myocardium in rats by regulating angiogenesis.…”

Section: Introductionmentioning

confidence: 99%

Proanthocyanidins Promote Endothelial Cell Viability and Angiogenesis

Wang

Mighri

et al. 2022

Journal of Cardiovascular Pharmacology

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Botanic drugs are reportedly effective in treating ischemic conditions by improving vascular circulation. However, it has been very rare for biomaterial researchers to look into the possibility of using such products in the context of tissue regeneration. This work studied 4 botanic drugs to explore their effects on vascular endothelial cell growth. Human umbilical endothelial cells were cultured in the presence of different doses of astragalus powder extract, astragalus injection, puerarin injection, and proanthocyanidin (PAC). Among the 4 drugs, PAC showed a potent effect on cell viability and stimulated cell growth in a dose-dependent manner. In particular, the PAC under test was able to maintain a high level of cell viability/proliferation comparable with the cells supplemented with the endothelial cell growth medium, at both low and normal serum conditions. Blocking either endothelial cell growth factor receptors or epithelial cell growth factor receptors was ineffective in reducing the stimulatory effect. The PAC released from polyvinyl alcohol cryogels stimulated HUVECs proliferation. The chick embryo chorioallantoic membrane model was further used to test the angiogenicity of PAC, showing that this botanic drug was potent in stimulating vasculature development. This work therefore demonstrates for the first time that PAC is capable of upregulating endothelial cell activity and growth in vitro in the absence of growth factors and that PAC can be loaded and released from drug carriers and can stimulate angiogenesis. These findings suggest the application of PAC in angiogenesis and tissue regeneration.

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Section: Introductionmentioning

confidence: 99%