The Effect of Corn-Expressed Carbohydrase on Performance and Digesta Viscosity of Broilers Fed a High Non-Starch Polysaccharide Diet

Ayres, V.E.; Baldwin, H.L.; Li, X.; Xu, Hengyong; Raab, R. Michael; Boney, J.W.; Broomhead, J. N.; Moritz, J. S.

doi:10.3382/japr/pfy049

Cited by 7 publications

(4 citation statements)

References 28 publications

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“…AC1 activity was tested in crude extracts from three representative AC1 crops (Lot numbers AV_AC1_0070, AV_AC1_0075, and AV_AC1_0077), AC1 that had been column-purified from AC1-expressing grain , and a commercially available thermostable β-glucanase (Sigma, catalog #G8673). All AC1 samples and the commercial β-glucanase samples were diluted using AC1 extraction buffer ( Ayres et al., 2018 ). The relative glucanase activity in each of the AC1 product batches and AC1 purified from AC1-expressing grain at the different temperatures was determined and are presented in Figure 1 .…”

Section: Resultsmentioning

confidence: 99%

“…This raises the question of whether diets incorporating an alternative source of dietary fiber such as barley or rye might demonstrate greater sensitivity to the effects of a glucanase. A study ( Ayres et al., 2018 ) examining the effects of AC1 in broiler diets containing 10 % DDGS and found that AC1 decreased viscosity of the digesta. Poultry appear to be more sensitive to supplemental carbohydrases than swine ( Adeola and Cowieson 2014 ), which may in part be due to the typically higher water content of the digesta in swine than in poultry, which makes swine less sensitive to changes in viscosity.…”

Section: Discussionmentioning

confidence: 99%

“…AC1 activity in grain or feed samples was carried out as described previously ( Ayres et al., 2018 ; Jasek et al., 2018 ). In this context, one unit (U) of AC1 activity equals 1 μmol/min glucose reducing equivalents released from 1 % barley-β-glucan at 80 °C, pH 6.5.…”

Section: Methodsmentioning

confidence: 99%

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Properties of corn-expressed carbohydrase AC1 in swine diets and its effects on apparent ileal digestibility, performance, hematology, and serum chemistry

Lessard

Broomhead³

et al. 2021

Heliyon

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Carbohydrases are often incorporated into livestock feed as digestive aids to improve animal performance. AC1 is a thermostable carbohydrase with β-1,4-glucanase, endo-cellulase, and cellobiohydrolase activity. AC1 has been expressed in corn, where it accumulates in the grain for easy inclusion in animal diets. Incorporating the enzyme in high-fiber diets (corn-soy supplemented with distiller's dry grains with solubles) that were fed to 5-week-old pigs led to a trend of decreasing viscosity of the digesta as the dose of the enzyme increased (P ¼ 0.092). AC1 also tended to increase the apparent ileal digestibility (AID) of neutral detergent fiber (P ¼ 0.076). When fed diets containing 2126 U/kg AC1, pigs experienced no adverse effects in terms of performance metrics (body weights, average daily gain, average daily feed intake and gain-to-feed ratio), hematology, blood chemistry or general health when compared to pigs fed a control diet that lacked AC1.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Properties of corn-expressed carbohydrase AC1 in swine diets and its effects on apparent ileal digestibility, performance, hematology, and serum chemistry

Lessard

Broomhead³

et al. 2021

Heliyon

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“…The digestive tract was then removed in its entirety, and digesta was manually extracted into a 50-mL centrifuge tube. The method used to prepare the digesta sampling was carried out according to Ayres et al (2018). The determination of digesta viscosity methodology used was carried out by the procedure suggested by Lamp et al (2015).…”

Section: Microbial Population In Cecal Content and Determination Of V...mentioning

confidence: 99%

Effect of Natural Multi-Enzyme Supplementation on Growth Performance, Gut Microflora, Carcass Characteristics, and Meat Quality of Broilers Reared in Tropical Climates

Soisuwan¹,

Plaimast²,

Thongnum³

et al. 2022

JAHP

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This study aimed to investigate the effect of natural multiple-enzyme supplementation on growth performance, gut microflora, carcass characteristics, and the meat quality of broilers reared in tropical climates. This study employed a completely randomized design (CRD). One thousand Ross 308 ® one-day-old chicks were divided into two treatments, each with 10 replicates and 50 chicks (25 females and 25 males) per experimental unit. Dietary treatment consisted of commercial broilers diets (corn-soybean based diet) supplemented with 0 or 100 g/ton of natural multi-enzymes feed supplementation (top-up feeding). Birds were provided ad libitum access to water and experimental diets. The results showed that natural multi-enzyme supplementation had no significant effect on the growth performances of broilers as compared to the control (P > 0.05). However, supplementation of natural multi-enzymes increased the viability rate of broilers during the starter period (P < 0.05). In addition, natural multi-enzyme supplementation exhibited positive effects on the productivity index, salable bird return, net profits return per bird, return on investment, and feed cost per gain compared to the control group (P < 0.05). Likewise, supplementation of natural multiple enzyme had no effect on the viscosity of the duodenal content and cecal microbial population, of broilers (P > 0.05). In addition, dietary treatments had no significant changes on carcass composition and meat quality (P > 0.05). Inclusion of 100 g/ton feed of natural multi-enzymes supplementation (top up feeding) of broiler chicken significantly affected the economic return of broiler production.

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Optimized soluble expression of a novel endoglucanase from Burkholderia pyrrocinia in Escherichia coli

Fan

Liao

et al. 2020

3 Biotech

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The Effect of Corn-Expressed Carbohydrase on Performance and Digesta Viscosity of Broilers Fed a High Non-Starch Polysaccharide Diet

Cited by 7 publications

References 28 publications

Properties of corn-expressed carbohydrase AC1 in swine diets and its effects on apparent ileal digestibility, performance, hematology, and serum chemistry

Properties of corn-expressed carbohydrase AC1 in swine diets and its effects on apparent ileal digestibility, performance, hematology, and serum chemistry

Effect of Natural Multi-Enzyme Supplementation on Growth Performance, Gut Microflora, Carcass Characteristics, and Meat Quality of Broilers Reared in Tropical Climates

Optimized soluble expression of a novel endoglucanase from Burkholderia pyrrocinia in Escherichia coli

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