The Effect of Cordycepin on Nuclear RNA Synthesis in Nerve and Glial Cells

Grahn, Birgitta; Løvtrup-Rein, Huguette

doi:10.1111/j.1748-1716.1971.tb04939.x

Cited by 12 publications

(3 citation statements)

References 18 publications

(6 reference statements)

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“…The insecticidal constituent of the C militaris fruiting body was identified as cordycepin. This is the first report on C militaris fruiting body‐derived cordycepin as a naturally occurring insecticidal compound against P xylostella larvae, although pharmacological or toxicological investigations have proved that it has antiviral,21 anti‐tumour,22 cytotoxic8 and anti‐protozoal23 activities as well as inhibiting RNA transcription24 and mRNA,25 and showing growth‐inhibiting activity against Clostridium perfringens 9 and Bacillus subtilis 26. Additionally, cordycepin has low toxicity to mammals 27…”

Section: Discussionmentioning

confidence: 93%

Larvicidal activity against Plutella xylostella of cordycepin from the fruiting body of Cordyceps militaris

Kim¹,

Yeon²,

Kim³

et al. 2002

Pest Management Science

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The insecticidal activities of methanol extracts of Cordyceps militaris Link (Ascomycotina: Clavicipitaceae) cultured on fresh pupae of Bombyx mori L against 3rd-instar larvae of Plutella xylostella L were examined using direct contact application. The larvicidal activity was more pronounced in an extract of C militaris fruiting body than in an extract of the pupae separated from the culture. The biologically active constituent of the Cordyceps fruiting body was characterized as cordycepin (3'-deoxyadenosine) by spectroscopic analysis. Responses varied according to dose, exposure time and application method. In a leaf-dipping test, cordycepin at 500 mg litre-1 caused no mortality at 1 DAT (day after treatment) but 78 and 100% mortality at 2 and 4 DAT, respectively, whereas 34 and 88% mortality at 3 and 5 DAT, respectively was observed at 300 mg litre-1. Cordycepin caused body colour change from pale green to dark brown and eventually body lysis. These results suggested that the larvicidal action may be attributable to a direct effect rather than an inhibitory action on chitin synthesis. There was a significant difference in insecticidal activity of cordycepin between leaf dipping (500 mg litre-1) with 100% mortality and topical application (10 micrograms per larva) with 0% mortality, suggesting that cordycepin has stomach action. Cordycepin merits further study as a potential P xylostella control agent or as a lead compound.

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Section: Discussionmentioning

confidence: 93%

Larvicidal activity against Plutella xylostella of cordycepin from the fruiting body of Cordyceps militaris

Kim¹,

Yeon²,

Kim³

et al. 2002

Pest Management Science

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“…However, tetracycline and chloramphenicol strongly inhibit growth of these lactic acid-producing bacteria, indicating alteration of intestinal microbiota which result in abnormal physiological state. This is the first report on C. militaris-derived cordycepin as a naturally occurring antibacterial compound toward these clostridia, although pharmacological investigations have proved that it has antiviral activity against HIV-1 in vitro (Mueller et al, 1991), antitumor activities (Penman et al, 1970), inhibition of RNA (Grahn and Loevtrup-Rein, 1971), and antiprotozoal activity (Trigg et al, 1971). Selective growth inhibition toward C. perfringens was also reported in oriental medicinal plant-derived materials such as polyphenols from Thea sinensis (Ahn et al, 1990b;Ahn et al, 1991) and Panax ginseng extract (Ahn et al, 1990a).…”

Section: Discussionmentioning

confidence: 96%

Cordycepin: Selective Growth Inhibitor Derived from Liquid Culture of Cordyceps militaris against Clostridium spp.

Ahn

Park

Lee

et al. 2000

J. Agric. Food Chem.

169

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The growth responses of nine human intestinal bacteria to liquid culture of Cordyceps militaris Link. Pt. (Ascomycotina: Clavicipitaceae) collected from a pupa of Bombyx mori L. (Lepidoptera: Bombycidae) were examined using spectrophotometric and impregnated paper disk methods and compared to those of tetracycline and chloramphenicol, as well as those of Coptis japonica root-derived berberine chloride. The biologically active constituent of the cultures was characterized as cordycepin (3'-deoxyadenosine) by spectroscopic analysis. This compound revealed potent growth-inhibiting activity toward Clostridium paraputrificum and Clostridium perfringens at 10 microgram/disk without adverse effects on the growth of Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium longum, Bifidobacterium adolescentis, Lactobacillus acidophilus, and Lactobacillus casei, whereas tetracycline and chloramphenicol inhibited the growth of these lactic acid-producing bacteria, clostridia and Escherichia coli. However, C. militaris-derived materials revealed no growth stimulation on the bifidobacteria and lactobacilli. These results may be an indication of at least one of the pharmacological actions of C. militaris. As a naturally occurring antibacterial agent, cordycepin could be useful as a new preventive agent against various diseases caused by clostridia.

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“…8 Submerged cultivated C. militaris is found to secrete cordycepin into the environment. 20 Cordycepin is found to possess antitumor activity, 21 inhibition of nuclear RNA synthesis in nerve and glial cells, 22 and antiviral activity towards HIV-1 in vitro, 23 as well as antiprotozoal effect 24 and insecticidal activity against Plutella xylostella (L.). 25 Structure-growth inhibition relationships in intestinal organisms have been studied.…”

Section: Discussionmentioning

confidence: 99%

Comparison of growth‐inhibiting activities of Cordyceps militaris and Paecilomyces japonica cultured on Bombyx mori pupae towards human gastrointestinal bacteria

2006

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The growth-inhibiting activities of materials derived from the fruiting body of Cordyceps militaris cultured on Bombyx mori pupae (CM-1) and pupae separated from the culture (CM-2), and the fruiting body of Paecilomyces japonica cultured on B. mori pupae (PJ-1) and pupae separated from the culture (PJ-2), fresh B. mori larvae (BML), fresh B. mori female pupae (BMP), and Morus alba leaves (MAL) towards eight lactic acid-producing bacteria and 11 harmful intestinal bacteria were examined using an impregnated paper disc bioassay. At 10 mg per disc, methanolic extracts from CM-1 and CM-2 strongly inhibited growth of Clostridium difficile ATCC 9689, C. paraputrificum ATCC 25780, and C. perfringens ATCC 13124 without adverse effects on the growth of eight lactic acid-producing bacteria and the other eight harmful bacteria. The methanolic extracts from PJ-1, PJ-2, BML, BMP, and MAL did not affect growth of all test bacteria. The growth-inhibiting principle of CM-1 and CM-2 towards test clostridia was characterized as cordycepin by spectroscopic analysis. The contents of cordycepin in dried CM-1 and CM-2 were 0.69% and 0.54%, respectively. These results suggest that cordycepin may be produced from the fruiting body of C. militaris cultured on B. mori pupae and then translocated to its host insect and accumulated. Structure-growth inhibition relationships of cordycepin and its eight derivatives against C. perfringens ATCC 13124 indicate that a deoxy form at either 3 or 2 position appears critical for the inhibitory activity. Natural cordycepin and its two analogues, 2 -deoxyadenosine and tubercidin, merit further study as a potential antibacterial agent against various diseases caused by harmful intestinal bacteria such as clostridia.

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The Effect of Cordycepin on Nuclear RNA Synthesis in Nerve and Glial Cells

Cited by 12 publications

References 18 publications

Larvicidal activity against Plutella xylostella of cordycepin from the fruiting body of Cordyceps militaris

Larvicidal activity against Plutella xylostella of cordycepin from the fruiting body of Cordyceps militaris

Cordycepin: Selective Growth Inhibitor Derived from Liquid Culture of Cordyceps militaris against Clostridium spp.

Comparison of growth‐inhibiting activities of Cordyceps militaris and Paecilomyces japonica cultured on Bombyx mori pupae towards human gastrointestinal bacteria

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