The effect of conjugation to gold nanoparticles on the ability of low molecular weight chitosan to transfer DNA vaccine

Zhou, Xianfeng; Zhang, Xizhen; Yu, Xianghui; Zha, Xiao; Fu, Qiuan; Liu, Bin; Wang, Xueyun; Chen, Yan; Chen, Yue; Shan, Yaming; Wu, Yongge; Kong, Wei; Shen, Jiacong

doi:10.1016/j.biomaterials.2007.09.007

Cited by 113 publications

(62 citation statements)

References 23 publications

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“…Moreover, GNPs are bioinert and nontoxic (30). Various GNPs functionalized with cationic quaternary ammonium (20), cationic lipid (21), chitosans (22), amino acids (23), and polyethylenimine (24) were demonstrated to be excellent vehicles for the delivery of genetic material into cells. Thomas and Klibanov covalently conjugated branched PEI of 2 kDa to GNPs and found that conjugation to GNPs greatly enhances PEI's transfer of plasmid DNA into mammalian cells (24).…”

Section: Introductionmentioning

confidence: 99%

Low Molecular Weight Polyethylenimine Conjugated Gold Nanoparticles as Efficient Gene Vectors

et al. 2010

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Gold nanoparticles (GNPs) conjugated with low molecular weight polyethylenimine (PEI 800 Da) were synthesized, and their characteristics as gene transfection vectors were investigated. The polyethylenimine conjugated GNPs (GNP-PEI800s) can retard plasmid DNA completely at N/P ratios above 4 in electrophoresis on agarose gel, and they also render effective protection of DNA from attack by DNase. TEM imaging revealed that GNP-PEI800s with higher PEI grafting density resulted in more compact and smaller complexes with plasmid DNA, compared to those obtained with lower grafting density ones. These complexes showed high efficiency in gene delivery in monkey kidney cells in vitro. In the absence of serum, GNP-PEI800s can transfect pGL-3 to COS-7 cells 3 to 4 orders more efficient than unmodified PEI800, reaching almost the same magnitude of PEI 25 kDa. More importantly, in contrast to the dramatically lowered efficiency of high molecular weight PEIs such as PEI 25 kDa in the presence of serum, the efficiency of GNP-PEI800s can be retained or even enhanced in serum-containing media. GNP-PEI800 1.3 exhibited transfection efficiency exceeding 60-fold that of PEI 25 kDa in 10% serum medium. All GNP-PEI800s exhibit mild cytotoxicity in comparison with that of PEI 25 kDa.

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Section: Introductionmentioning

confidence: 99%

Low Molecular Weight Polyethylenimine Conjugated Gold Nanoparticles as Efficient Gene Vectors

et al. 2010

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“…The promising properties of gold particle filled Cs matrix has also encouraged some research works to fabricate and investigate the Cs/Au composites as potential materials for drug delivery application [81]. The stabilization that was induced by Au nano particles towards Cs matrix produced a better drug loading and stability of Cs composites [92,93]. Meanwhile, the presence of Au nano particles in Cs matrix improved the radiotherapy, whereby the drug can be delivered locally to the targeted tumour sites [94,95].…”

Section: Cs/metal Biocompositesmentioning

confidence: 97%

Preparation, Properties and Applications of Chitosan-Based Biocomposites/Blend Materials: A Review

Julkapli

Akil

Ahmad

2011

Composite Interfaces

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“…Zhou et al (2008) demonstrated that gold nanoparticles conjugated with low molecular weight chitosan induce an enhanced serum antibody response ten-times more potent than naked DNA vaccine. Park et al (2013) reviewed the role of nanotechnology in the field of immunotherapy.…”

Section: Gold Nanoparticlesmentioning

confidence: 98%

Fungi as an efficient mycosystem for the synthesis of metal nanoparticles: progress and key aspects of research

et al. 2015

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Nanotechnology is an emerging cutting-edge technology, which involves interdisciplinary subjects, such as physics, chemistry, biology, material science and medicine. Different methods for the synthesis of nanoparticles have been discussed here. Although physical and chemical methods have been successfully used to synthesize nanoparticles, the use of hazardous chemicals and synthesis at high temperature is a matter of concern. Hence, there is a necessity to develop eco-friendly techniques for the synthesis of nanoparticles. Biosynthesis of nanoparticles by fungi, bacteria, actinomycetes, lichen and viruses have been reported eco-friendly. Moreover, the fungal system has emerged as an efficient system for nanoparticle synthesis as fungi possess distinctive characters including high wall binding capacity, easy to culture and simpler biomass handling, etc. In this review, we have discussed fungi as an important tool for the fabrication of nanoparticles. In addition, methods and mechanism for synthesis of nanoparticles and its potential applications have also been discussed.

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The effect of conjugation to gold nanoparticles on the ability of low molecular weight chitosan to transfer DNA vaccine

Cited by 113 publications

References 23 publications

Low Molecular Weight Polyethylenimine Conjugated Gold Nanoparticles as Efficient Gene Vectors

Low Molecular Weight Polyethylenimine Conjugated Gold Nanoparticles as Efficient Gene Vectors

Preparation, Properties and Applications of Chitosan-Based Biocomposites/Blend Materials: A Review

Fungi as an efficient mycosystem for the synthesis of metal nanoparticles: progress and key aspects of research

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