The effect of conjugated linoleic acid on transepithelial calcium transport and mediators of paracellular permeability in human intestinal-like Caco-2 cells

Jewell, Christopher M.; Cusack, Siobhán; Cashman, Kevin D.

doi:10.1016/j.plefa.2004.10.015

Cited by 30 publications

(20 citation statements)

References 30 publications

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“…The concentrations of FAs used were much lower than those present in food and similar to the concentrations present in the lumen of the human small intestine [32,33]. We extend earlier work by not only comparing effects on acute CCK secretion, but also cellular CCK content, CCK mRNA, cellular DNA synthesis, cell viability and cell toxicity.…”

Section: Introductionsupporting

confidence: 52%

Acute and chronic effects of dietary fatty acids on cholecystokinin expression, storage and secretion in enteroendocrine STC‐1 cells

Hand

Bruen

O’Halloran

et al. 2010

Molecular Nutrition Food Res

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Cholecystokinin (CCK) is a peptide hormone secreted from the I-cells of the intestine and it has important physiological actions related to appetite regulation and satiety. In this study we used STC-1 cells to investigate the effects of common dietary-derived fatty acids (FAs) on I-cell secretory function and metabolism. We extend earlier studies by measuring the acute and chronic effects of 11 FAs on CCK secretion, cellular CCK content, CCK mRNA levels, cellular DNA synthesis, cellular viability and cytotoxicity. FAs were selected in order to assess the importance of chain length, degree of saturation, and double bond position and conformation. The results demonstrate that secretory responses elicited by dietary FAs are highly selective. For example, altering the conformation of a double bond from cis to trans (i.e. oleic acid versus elaidic acid) completely abolishes CCK secretion. Lauric acid appears to adversely affect I-cell metabolism and arachidonic acid suppresses DNA synthesis. Our studies reveal for the first time that conjugated linoleic acid isoforms are particularly potent CCK secretagogues, which also boost intracellular stores of CCK. These actions of conjugated linoleic acid may explain satiating actions observed in dietary intervention studies.

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Section: Introductionsupporting

confidence: 52%

Acute and chronic effects of dietary fatty acids on cholecystokinin expression, storage and secretion in enteroendocrine STC‐1 cells

Hand

Bruen

O’Halloran

et al. 2010

Molecular Nutrition Food Res

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“…Oleic acid dispersed in the medium was partially absorbed into Caco2 cells and stimulated corresponding triacylglycerol re-synthesis at the level of 25%. This result was consistent with the previous ones that only oleic acid stimulated lipoprotein production by increasing the synthesis and accumulation of cellular triacyglycerol [12,25]. The other lipolysis product, 2-monoolein dispersed in the medium also facilitated the triacylglycerol re-synthesis in the Caco2 cells (27%).…”

Section: Effects Of the Components Of The Mixed Micelle On The Re-estsupporting

confidence: 92%

“…In the previous works, lipid metabolites were dispersed or emulsified in the medium containing serum albumin before being supplied to the Caco2 cells [10][11][12]. In this study, the mixed micelles were formed by physiological surfactants, Tau and LysoPC to monitor the epithelial transport of the lipid metabolites into the Caco2 cells from mixed micelles.…”

Section: Potential Cytotoxic Effects Of Tau and Lysopc On Caco2 Cellsmentioning

confidence: 99%

“…In the previous studies, specific fatty acids, such as unsaturated fatty acids, 13-hydroxy octadecadienoic, were isotope-labeled and served in the Caco2 cells to monitor their behaviors [5][6][7][8]. Non isotope-labeled fatty acids were used in this cells model, only when their effects on transcellular permeation of other components, ions, proteins and so on, had been evaluated [9][10][11][12]. Therefore, little information has been available for the comparative study of transepithelial transport of various structured lipid metabolites in the Caco2 cells, because the kinds of isotope-labeled lipids metabolites used in the experiments were limited.…”

Section: Introductionmentioning

confidence: 99%

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Absorption Properties of Micellar Lipid Metabolites into Caco2 Cells

Tsuzuki¹

2007

Lipids

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To elucidate the absorption characteristics of dietary lipids in the human intestine, we investigated the cellular uptake of lipid metabolites using a differential monolayer of the Caco2 cells. As lipid metabolites, several free fatty acids and 2-monoacylglycerols, were formed a mixed micelle by bile salts and lysophospholipids and they were supplied to the Caco2 cells. To estimate the effect of the mixed micelles on the permeability of cells' membranes during incubation with the mixed micelles, the transepitherial electrical resistance (TEER) value was monitored, and no pronounced changes of TEER was detected. This suggested that mixed micelles did not affect their cellular properties of the barrier measured by TEER. The lipid metabolites transferred from the mixed micelle into the Caco2 cells were determined quantitatively by an enzymatic colorimetric method and were done by thin layer chromatography (TLC) for a species of acylglycerols. These highly sensitive methods enabled us to monitor the transepithelial transports of various kinds of non-isotope-labeled various lipid metabolites. Newly re-synthesized triacylglycerols were accumulated in Caco2 cells after 30 min incubation with the mixed micelles, and their amounts increased gradually for 4 h. The secretion of re-esterified triacylglycerols into a basolateral medium from the Caco2 cells began at 2 h after the mixed micelles were added to the apical medium. The intake of external lipid metabolites by the Caco2 cells were evaluated by an initial 2-h incubation with the mixed micelles. For example, 2-monomyristin and 2-monopalmitin were more rapidly transferred into the Caco2 cells from the mixed micelles than 2-monocaprin was. On the other hand, the absorption rates of capric acid, lauric acid and myristic acid by the cells were larger than those of stearic acid and oleic acid. It revealed that the side-chain structure of these lipid metabolites affected their absorption by the Caco2 cells. The results of this study suggested that the Caco2 cell monolayer could be a useful model for investigating the involvement of dietary lipids in the transepithelial absorption in the human intestine.

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“…Overall Ca transport and transepithelial electrical resistance in Caco-2 cells were unaffected by exposure to any of the fatty acids for 24 h or 22 days, whereas paracellular (but not total transepithelial and transcellular) Ca 2+ transport across Caco-2 cells was significantly increased after 22 days [93]. Later, the same authors reported that both isomers of CLA increased overall transepithelial Ca 2+ transport as well as transcellular and paracellular Ca 2+ transport in monolayers of Caco-2 cells, an effect that could be related to changes in the zona occludens-1 (a tight junction protein) [94]. Recently, Murphy et al [95] investigated the molecular mechanisms underlying CLA-induced stimulation of Ca transport using microarray data together with quantitative reverse transcriptase-PCR analysis.…”

Section: Regulation By Nutrientsmentioning

confidence: 99%

Minireview on Regulation of Intestinal Calcium Absorption

Pérez¹,

Picotto²,

Carpentieri

et al. 2008

Digestion

130

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An overview of current information on the mechanisms by which intestinal calcium absorption occurs is described in this article. Both paracellular and transcellular pathways are analyzed. Special emphasis focuses on molecules participating in the latter pathway, such as TRPV5 and TRPV6 channels, located in the apical region of the enterocytes, CB_9k and CB_28k, presumably involved in the cation movement from the apical to the basolateral pole of the cell, and PMCA_1b and Na⁺/Ca²⁺ exchanger, proteins that extrude Ca²⁺ from the cells. Current concepts on the relative importance of paracellular and transcellular calcium transport and the vitamin D dependence of each pathway are referred and analyzed showing the contrasting views on this issue. More detailed information is given regarding the stimulatory effect of vitamin D on intestinal Ca²⁺ absorption either in animal models or in the human intestine. The possible mechanisms triggered by hormones such as PTH, calcitonin, estrogen, thyroid hormone, glucocorticoids and different nutritional factors on intestinal calcium absorption are also reviewed. Finally, the influence of physiological conditions such as growth, pregnancy, lactation and aging on intestinal calcium absorption are discussed.

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The effect of conjugated linoleic acid on transepithelial calcium transport and mediators of paracellular permeability in human intestinal-like Caco-2 cells

Cited by 30 publications

References 30 publications

Acute and chronic effects of dietary fatty acids on cholecystokinin expression, storage and secretion in enteroendocrine STC‐1 cells

Acute and chronic effects of dietary fatty acids on cholecystokinin expression, storage and secretion in enteroendocrine STC‐1 cells

Absorption Properties of Micellar Lipid Metabolites into Caco2 Cells

Minireview on Regulation of Intestinal Calcium Absorption

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