The effect of conjugated linoleic acid isomers on fatty acid profiles of liver and adipose tissues and their conversion to isomers of 16∶2 and 18∶3 conjugated fatty acids in rats

Sébédio, Jean‐Louis; Angioni, Elisabetta; Chardigny, Jean‐Michel; Grégoire, Stéphane; Juanéda, Pierre; Berdeaux, Olivier

doi:10.1007/s11745-001-0759-8

Cited by 97 publications

(93 citation statements)

References 43 publications

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“…The preferential esterification of rumenic acid and its derivatives into polar lipids and oleic acid into neutral lipids confirmed previous results in the lamb liver showing that CLA and its conjugated derivatives such as C18:3, C20:3 and even C20:4 are mainly associated with polar lipids [18]. This preferential path of conjugated FA esterification appeared to be specific to ruminant animals since in the rat liver rumenic acid was mainly incorporated into neutral lipids [21].…”

Section: Discussionsupporting

confidence: 88%

“…The presence of these derivatives has been mentioned in the liver of different species such as the rat [16][17][18]21], lamb [19] and mouse [20]. Moreover, it has been recently demonstrated in the rat that the liver is able to convert rumenic acid into a conjugated derivative identified on the basis of its retention time as the cis-6, cis-9, trans-11 C18:3 [21].…”

Section: Discussionmentioning

confidence: 99%

“…In such situations, large amounts of FA, including CLA which are stored in adipose tissues, are released into the blood and taken up by the liver. The intensity of the different CLA metabolic pathways in the liver is still poorly documented except for the fact that in the liver of different species such as the rat [16][17][18], lamb [19] and mouse [20] given CLA-supplemented diets, CLA is converted into conjugated C18:3 by ∆6 desaturase activity [2,17]. Although these studies reported the presence of CLA derivatives in the liver of animals, only Gruffat et al [21] recently demonstrated, by using rat liver slices incubated with cis-9, trans-11 and trans-10, cis-12 CLA isomers, that CLA conversion into conjugated C18:3 actually occurs in this organ.…”

Section: Introductionmentioning

confidence: 99%

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In vitro metabolism of rumenic acid in bovine liver slices

et al. 2005

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-Ruminant products are the major source of CLA for humans. However, during periods of fat mobilization, the liver might play an important role in CLA metabolism which would limit the availability of the latter for muscles and milk. In this context, rumenic acid (cis-9, trans-11 CLA) metabolism in the bovine liver (n = 5) was compared to that of oleic acid (n = 3) by using the in vitro liver slice method. Liver slices were incubated for 17 h in a medium containing 0.75 mM of FA mixture and 55 µM of either [1-14 C] rumenic acid or [1-14 C] oleic acid at 37 °C under an atmosphere of 95% O 2 -5% CO 2 . Rumenic acid uptake by liver slices was twice (P = 0.009) that of oleic acid. Hepatic oxidation of both FA (> 50% of incorporated FA) led essentially to the production of acidsoluble products and to a lower extent to CO 2 production. Rumenic acid was partly converted (> 12% of incorporated rumenic acid) into conjugated C18:3. CLA and its conjugated derivatives were mainly esterified into polar lipids (71.7%), whereas oleic acid was preferentially esterified into neutral lipids (59.8%). Rumenic acid secretion as part of VLDL particles was very low and was onefourth lower than that of oleic acid. In conclusion, rumenic acid was highly metabolized by bovine hepatocytes, especially by the oxidation pathway and by its conversion into conjugated C18:3 for which the biological properties need to be elucidated. rumenic acid / oleic acid / metabolism / liver / bovine Abbreviations: ASP, acid-soluble products; BSA, bovine serum albumin; CLA, conjugated linoleic acid; FA, fatty acids; NL, neutral lipids; PL, polar lipids; VLDL, very-low density lipoproteins.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In vitro metabolism of rumenic acid in bovine liver slices

et al. 2005

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“…Phospholipids, cholesteryl esters, and TG were separated by TLC on silica plates (Merck, Darmstadt, Germany). Their constitutive FAs were methylated according to the procedure of Christie, Sebedio, and Juaneda (16) and analyzed by gas-liquid chromatography as described previously (17). For skeletal muscle and heart, aliquots of total lipid extracts were resuspended in a solution of Triton X-100 as described previously (18).…”

Section: Lipid Analysismentioning

confidence: 99%

Upregulation of liver VLDL receptor and FAT/CD36 expression in LDLR−/− apoB100/100 mice fed trans-10,cis-12 conjugated linoleic acid

Degrace

Moindrot

Mohamed

et al. 2006

Journal of Lipid Research

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This study explores the mechanisms responsible for the fatty liver setup in mice fed trans-10,cis-12 conjugated linoleic acid (t10c12 CLA), hypothesizing that an induction of low density lipoprotein receptor (LDLR) expression is associated with lipid accumulation. To this end, the effects of t10c12 CLA treatment on lipid parameters, serum lipoproteins, and expression of liver lipid receptors were measured in LDLR 2/2 apoB 100/100 mice as a model of human familial hypercholesterolemia itself depleted of LDLR. Mice were fed t10c12 CLA over 2 or 4 weeks. We first observed that the treatment induced liver steatosis, even in the absence of LDLR. Mice treated for 2 weeks exhibited hypertriglyceridemia with high levels of VLDL and HDL, whereas a 4 week treatment inversely induced a reduction of serum triglycerides (TGs), essentially through a decrease in VLDL levels. In the absence of LDLR, the mRNA levels of other proteins, such as VLDL receptor, lipoprotein lipase, and fatty acid translocase, usually not expressed in the liver, were upregulated, suggesting their involvement in the steatosis setup and lipoprotein clearance. The data also suggest that the TG-lowering effect induced by t10c12 CLA treatment was attributable to both the reduction of circulating free fatty acids in response to the severe lipoatrophy and the high capacity of liver to clear off plasma lipids.-Degrace, P., B. Moindrot, I. Mohamed, J. Gresti, Z-Y. Du, J-M. Chardigny, J-L. Sébédio, and P. Clouet. Upregulation of liver VLDL receptor and FAT/ CD36 expression in LDLR 2/2 apoB 100/100 mice fed trans-10,cis-12 conjugated linoleic acid.

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“…Park et al (1997) observed a reduction in the degree of obesity in mice given CLA. Namely, CLA promotes anabolic and suppresses catabolic processes, facilitates the incorporation of protein into muscle tissue, and reduces body fat accumulation in several animal models (DeLany and West, 2000;Pariza et al, 2000;Sebedio, 2001;Badinga et al, 2001).…”

mentioning

confidence: 99%

In sacco studies of conjugated linoleic acid production from various oils in the rumen of sheep

Szöllőskei

Wagner²,

Németh³

et al. 2005

Acta Veterinaria Hungarica

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In this experiment sunflower oil, soybean oil and fish oil were incubated in rumen-fistulated adult ewes (n = 5) to study conjugated linoleic acid (CLA) production in the rumen. The individual oils were incubated in nylon bags in the rumen on perlite carrier (40% oil, 60% carrier) over a period of 2, 4, 6, 8, 10 and 12 h for all treatments. During the incubation of each oil primarily the formation of the cis-9, trans-11 isomer of CLA could be observed. Both sunflower and soybean oils showed similar changes in the rumen. After the incubation of these two vegetable oils the proportion of linoleic acid decreased significantly as the duration of incubation increased in the rumen. These changes were accompanied by a significant increase in the amount of cis-9, trans-11 CLA. However, in the case of sunflower oil the rate of formation of the cis-9, trans-11 CLA isomer was significantly higher after the different incubation times as compared to soybean oil. Much lower amounts of CLA were formed when fish oil was incubated in the rumen. The level of cis-9, trans-11 isomer produced during these treatments was 10% less than the amount obtained with the other two oils of vegetable origin. Besides the cis-9, trans-11 isomer, trans-10, cis-12 CLA could also be detected during the incubation of the different oils in the rumen. However, the level of this isomer was low and did not show consistent differences among the treatments. The results of this experiment indicate that the fatty acid composition of the oils and the duration of incubation collectively determine the amount of CLA produced in the first compartment of the forestomach of ruminants.

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The effect of conjugated linoleic acid isomers on fatty acid profiles of liver and adipose tissues and their conversion to isomers of 16∶2 and 18∶3 conjugated fatty acids in rats

Cited by 97 publications

References 43 publications

In vitro metabolism of rumenic acid in bovine liver slices

In vitro metabolism of rumenic acid in bovine liver slices

Upregulation of liver VLDL receptor and FAT/CD36 expression in LDLR−/− apoB100/100 mice fed trans-10,cis-12 conjugated linoleic acid

In sacco studies of conjugated linoleic acid production from various oils in the rumen of sheep

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