The micromechanics of isolated tomato fruit cells were investigated by microcompression-holding. Covering the cells with deionized water after isolation caused no significant volume changes, suggesting that cells suspended in water for compression testing were representative of those in the original tissue. The viscoelastic-plastic behavior of such cells was characterized by compression at 4900 ± 200 μm s-1 , then holding. Although the cells were generally not spherical initially and some cell deformation appeared to be local, the force-time data were fitted by the Hertz-Maxwell model for relaxation of viscoelastic spheres. The force at 15% deformation, instantaneous and equilibrium elastic moduli, yield strength, and first and second relaxation times were 2.5 ± 0.6 mN, 0.6 ± 0.3 MPa, 0.22 ± 0.08 MPa, 0.03 ± 0.01 MPa, 0.48 ± 0.05 s, 0.033 ± 0.004 s, respectively. These parameters showed little sensitivity to several reasonable definitions of cell size nor to changes in the (assumed) Poisson's ratio. Industrial Relevance: Fresh fruit is very susceptible to damage during industrial handling (e.g. mechanical harvesting, packaging and transport). Mechanical damage to fruit, manifested at the macro scale, is caused ultimately by failure of cells at the micro scale. Viscoelastic-plastic characterization of single cells isolated from tissue is vital to macro-scale modelling, simulation and prediction of mechanical damage to fruits. The method might be extended to single cells of other fruits.