The effect of complex carbon and nitrogen, salt, Tween-80 and acetate on delta-endotoxin production by a Bacillus thuringiensis subsp kurstaki

Zouari, Nabil; Jaoua, Samir

doi:10.1038/sj.jim.2900756

Cited by 30 publications

(49 citation statements)

References 11 publications

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“…In this case, LC 50 values obtained with medium 05 were significantly lower than with medium 02. Results from medium 05 are consistent with previous studies indicating the importance of the nitrogen source in improving the synthesis of entomopathogenic crystals (IÇGEN et al, 2002b;KHUZHAMSHUKUROV et al, 2001;ÖZKAN et al, 2003;ZOUARI;JAOUA 1999). Table 3 -Effects of medium composition on the growth of B. thuringiensis var.…”

Section: Resultssupporting

confidence: 81%

Desenvolvimento de um meio de cultura de baixo custo para Bacillus thuringiensis subsp. israelensis

Angelo

Vilas-Bôas

Santos

et al. 2013

Semin. Cienc. Biol. Saude

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Section: Resultssupporting

confidence: 81%

Desenvolvimento de um meio de cultura de baixo custo para Bacillus thuringiensis subsp. israelensis

Angelo

Vilas-Bôas

Santos

et al. 2013

Semin. Cienc. Biol. Saude

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“…3 In the present work, we studied fermentation conditions in 2-and 20-dm 3 fermenters, for the lowcost production of bioinsecticides, using complex media based on gruel and ®sh meal. Several major parameters related to physical conditions and media composition were investigated.…”

Section: Introductionmentioning

confidence: 99%

Production of delta‐endotoxin by Bacillus thuringiensis subsp kurstaki and overcoming of catabolite repression by using highly concentrated gruel and fish meal media in 2‐ and 20‐dm³ fermenters

Zouari¹,

Achour²,

Jaoua³

2002

J of Chemical Tech & Biotech

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Delta-endotoxin production by a strain of Bacillus thuringiensis subsp kurstaki exhibiting larvicidal toxicity towards lepidoptera was investigated in 2-and 20-dm 3 fermenters, using gruel-and ®sh meal-based media. The results show clearly that in such complex media, aeration plays an important role in bioinsecticide production. Optimal aeration led to improvement of delta-endotoxin concentrations with decreases of ®nal spore count and proteolytic activity. Moreover, in order to use high gruel concentrations, a fermenter con®guration with an ef®cient aeration system should be used. In a 20-dm 3 Biola®te fermenter, 59 g dm À3 or 75 g dm À3 gruel was used to produce bioinsecticides with a signi®cant reduction of carbon catabolite repression of delta-endotoxin synthesis. This result is very interesting in order to produce high ®nal delta-endotoxin concentrations in the culture broth. It was also concluded, by considering the key role of oxidative pathways in delta-endotoxin synthesis, that oxygen supply must be adequate for bioinsecticide production at high substrate concentrations. Moreover, the role of sodium chloride in improving delta-endotoxin production is dependent not only on protease synthesis and its effect on crystal stability, but also on the aeration level of the production medium.

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“…Como fontes alternativas, vêm se destacando farinhas derivadas de proteína animal (farinha de peixe e de crisálida) e alguns cereais (cevada e trigo) (ALVES et al, 1997;DEVI;RAVINDER;JAIDEV, 2005;GHRIBI;JAOUA, 2005;MORRIS;DEAN, 1997;PRABAKARAN;BALAMARAN, 2006;JAOUA, 1999).…”

Section: Segundo Deunclassified

Bacillus thuringiensis: características gerais e fermentação

2010

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ResumoO controle de insetos é realizado, em sua maioria, por produtos químicos; cujos efeitos cumulativos ocasionam grandes prejuízos ambientais e à saúde humana, destacando-se ainda a rápida seleção de insetos resistentes. O controle biológico por entomopatógenos é uma alternativa eficiente, principalmente devido a sua alta especificidade, ausência de resistência nos insetos alvos e baixo efeito residual no ambiente. Bacillus thuringiensis é uma bactéria Gram-positiva esporulante, produtora de cristais protéicos com atividade inseticida. Apesar do amplo uso B. thuringiensis no controle biológico, há poucos trabalhos publicados quanto a sua multiplicação, esporulação e produção de cristais, visto que muitas informações são segredos industriais. Esta revisão teve por objetivo reunir informações gerais sobre B. thuringiensis, bem como quanto sua produção em meios de cultivo. Palavras-chave: Bacillus thuringiensis, entomopatógeno, bioinseticida, controle biológico, fermentação AbstractThe insect control is carried out mostly by chemical products, whose cumulative effects cause serious losses to environmental and human health, highlighting rapid selection of resistant insects. Biological control by entomopathogenic bacteria is an efficient alternative, mainly due to high specificity, absence of resistance in the target insects and low environment residual effect. Bacillus thuringiensis is a Grampositive spore-forming bacterium that produces a parasporal crystal protein toxic for many insect species. Despite the widespread use of B. thuringiensis based products in biological control of insects, there are little studies about its multiplication, sporulation and crystal production, as so many trade secrets information. This revision was aimed at gathering general information about B. thuringiensis, as well as its production in culture media.

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The effect of complex carbon and nitrogen, salt, Tween-80 and acetate on delta-endotoxin production by a Bacillus thuringiensis subsp kurstaki

Cited by 30 publications

References 11 publications

Desenvolvimento de um meio de cultura de baixo custo para Bacillus thuringiensis subsp. israelensis

Desenvolvimento de um meio de cultura de baixo custo para Bacillus thuringiensis subsp. israelensis

Production of delta‐endotoxin by Bacillus thuringiensis subsp kurstaki and overcoming of catabolite repression by using highly concentrated gruel and fish meal media in 2‐ and 20‐dm³ fermenters

Bacillus thuringiensis: características gerais e fermentação

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The effect of complex carbon and nitrogen, salt, Tween-80 and acetate on delta-endotoxin production by a Bacillus thuringiensis subsp kurstaki

Cited by 30 publications

References 11 publications

Desenvolvimento de um meio de cultura de baixo custo para Bacillus thuringiensis subsp. israelensis

Desenvolvimento de um meio de cultura de baixo custo para Bacillus thuringiensis subsp. israelensis

Production of delta‐endotoxin by Bacillus thuringiensis subsp kurstaki and overcoming of catabolite repression by using highly concentrated gruel and fish meal media in 2‐ and 20‐dm3 fermenters

Bacillus thuringiensis: características gerais e fermentação

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Production of delta‐endotoxin by Bacillus thuringiensis subsp kurstaki and overcoming of catabolite repression by using highly concentrated gruel and fish meal media in 2‐ and 20‐dm³ fermenters