The Effect of Combining Post-Harvest Calcium Nanoparticles with a Salicylic Acid Treatment on Cucumber Tissue Breakdown via Enzyme Activity during Shelf Life

Abstract

In the present study, an experiment was carried out on the postharvest of cucumber fruit during a 14-day shelf life. The aim was to assess the impact of calcium nanoparticles (CaNPs) blended with different concentrations of salicylic acid (SA) on the shelf life of cucumbers during the seasons of 2018 and 2019. The investigation further monitored the influences of CaNPs-SA on some physical properties of cucumber, including the percentage weight loss, color, and fruit firmness. In addition, chemical properties, such as total soluble solids (SSC%), total acidity (TA%), total soluble sugars, and chlorophyll pigmentation of the fruit skin, were assessed during a 14-day shelf lifeCell wall degradation enzymes (CWEAs) such as polygalacturonase (PG), cel-lulase (CEL), xylanase (XYL), and pectinase (PT) were also researched. In addition, the generation rates of H2O2 and O2•− were calculated, as well as the reduction of DPPH. The lipid peroxidation (malondialdehyde, MDA) and cell membrane permeability (IL%) of cell wall composites were also determined. CaNPs-SA at 2 mM suppressed CWEAs, preserved fruit quality, reduced weight loss throughout the shelf-life period, and reduced the percent leakage value. At this concentration, we also found the lowest levels of MDA and the highest levels of DPPH.

Calcium Nanoparticles Intermixed with Salicylic Acid Affect Enzymes Activities and Postharvest Attributes of Cut Rose (Rosa hybrida Cv. Black Magic)

“…The current study showed that intermixed CaNPs at 2 mM with SA delayed cell wall degrading by modulating XYL, PG, CEL, and PT, and promoting the production of antioxidant enzyme activity (CAT) (Figure 9) which decreased the MDA relative to the control (Figure 10A) over the vase life period (Youwei and Yinzhe, 2013) and increase CAT activity could aid in the scavenging of H2O2 during the vase life period (Abdelkader et al, 2022). Additionally, the presence of Ca +2 causes the middle lamella to degrade by lowering the activity of the polygalacturonase enzyme (Wehr et al, 2004).…”