The effect of collagen on the cyclic AMP content of embryonic somites

Kosher, Robert A.; Savage, Mary P.

doi:10.1002/jez.1402080105

Cited by 10 publications

(1 citation statement)

References 23 publications

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“…A variety of agents that elevate intracellular cyclic AMP levels inhibit formation of the small amount of cartilage normally formed by somites in vitro in the absence of inducing tissues and inhibit the ability of somites to respond to the inductive influence of the notochord or collagen (Kosher, 1976). In addition, collagen-induced in vitro somite chondrogenesis is accompanied by reduction in the cyclic AMP content of the expiants (Kosher and Savage, 1979).…”

Section: E the Regulation Of Somitementioning

confidence: 98%

The Chondroblast and the Chondrocyte

Kosher¹

1983

Cartilage

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Section: E the Regulation Of Somitementioning

confidence: 98%

The Chondroblast and the Chondrocyte

Kosher¹

1983

Cartilage

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Cyclic AMP derivatives stimulate the chondrogenic differentiation of the mesoderm subjacent to the apical ectodermal ridge of the chick limb bud

Kosher¹,

Savage²,

Chan³

1979

J. Exp. Zool.

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Recent studies indicate that one of the major functions of the apical ectodermal ridge (AER) of the embryonic chick limb bud is to maintain mesenchymal cells directly subjacent to it (i.e., cells extending 0.4-0.5 mm from the AER) in a labile, undifferentiated condition. Furthermore, when mesenchymal cells are freed from the AER's influence, either artifically or as a result of normal polarized proximal-to-distal limb outgrowth, they are freed to commence cytodifferentiation. In a preliminary attempt to investigate at a molecular level the mechanism by which the AER exerts its "negative" effect on the cytodifferentiation of subridge mesenchymal cells, we have examined the effect of a variety of agents that elevate cyclic AMP levels on the chondrogenic differentiation of the unspecialized subridge mesoderm of the limb bud in an organ culture system. Dibutyryl- and 8-hydroxy-cyclic AMP elicit a dose-dependent increase in the rate and amount of cartilage matrix formation and a corresponding dose-dependent increase in sulfated glycosaminoglycan accumulation by subridge mesoderm explants. The stimulatory effect of suboptimal concentrations of cyclic AMP derivatives is potentiated by the addition of theophylline. The stimulatory effect is limited to cyclic AMP derivatives, since dibutyryl-cyclic GMP and 5'-AMP have no effect. Thus agents that elevate intracellular cyclic AMP levels stimulate the chondrogenic differentiation of the unspecialized subridge mesoderm of the embryonic chick limb bud.

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Immunohistochemical localization of cyclic AMP during normal and abnormal chick and mouse limb development

1981

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This paper describes the immunohistochemical localization of cAMP during limb chondrogenesis in talpid3 chick, brachypod mouse, and normal embryos. Comparisons were made between chick wing buds at Stages 22, 25, and 30, and mouse hind limb buds at Days 11, 12.5 and 14. At Stage 22, the normal mesenchyme in the chick displayed areas of bright fluorescence compared to a lesser intense and more evenly distributed fluorescence in talpid3. Sections of the central region from normal Stage 25 limb buds exhibited an intense fluorescence that was uniformly distributed, whereas, in talpid3 staining was more mosaic with some areas fluorescing brightly and others showing little fluorescence. At Stage 30 the staining pattern was similar between normal and talpid3, with the fluorescence being brighter in the cartilage tissue than in the surrounding soft tissue. Difference in the staining patterns of normal and brachypod limb tissue were not detectable. At Days 11 and 12.5, tissue from both genotypes displayed a very bright, uniform fluorescence. In the 14-day hind limb buds, the staining patterns were comparable to those observed in Stage 30 chick wing buds. However, under in vitro conditions conducive for the expression of the chondrogenic phenotype, differences in the intensity and extensiveness of fluorescent staining were detectable in cultures derived from 12-day normal and brachypod hind limb mesenchyme. Compared to the control, the uneven distribution of immunofluorescence in the talpid3 limb buds and the differences in intensity and extensiveness of fluorescence in the brachypod cultures support the hypothesis that cAMP is involved in limb cartilage differentiation.

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The effect of collagen on the cyclic AMP content of embryonic somites

Cited by 10 publications

References 23 publications

The Chondroblast and the Chondrocyte

The Chondroblast and the Chondrocyte

Cyclic AMP derivatives stimulate the chondrogenic differentiation of the mesoderm subjacent to the apical ectodermal ridge of the chick limb bud

Immunohistochemical localization of cyclic AMP during normal and abnormal chick and mouse limb development

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