The effect of collagen-binding NGF-β on the promotion of sciatic nerve regeneration in a rat sciatic nerve crush injury model

Sun, Wenjie; Sun, Changkai; Lin, Hang; Zhao, Hui; Wang, Jingyu; Ma, Hui; Chen, Bing; Xiao, Zhifeng; Dai, Jianwu

doi:10.1016/j.biomaterials.2009.05.037

Cited by 117 publications

(80 citation statements)

References 37 publications

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“…However, the hydrophobicity and deficient biological activity of polyesters such as PCL are unfavorable for adhesion of cells and their proliferation [7]. Various biological activators, such as laminin and nerve growth factors (NGF), have been adopted to modify PCL to enhance its biological activity for various applications, including nerve guidance conduits [8,9]. Tirofiban (TF) is a non-peptide RGD-mimetic molecule [10] that is produced from the snake venom, echistatin and has a function that is analogous to that of arginine-glycine-aspartic acid (RGD), a wellknown minimal cell-recognizable peptide of numerous adhesive plasma and extracellular proteins such as fibronectin [7,10,11].…”

Section: Introductionmentioning

confidence: 99%

Promoting regeneration of peripheral nerves in-vivo using new PCL-NGF/Tirofiban nerve conduits

et al. 2011

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Section: Introductionmentioning

confidence: 99%

Promoting regeneration of peripheral nerves in-vivo using new PCL-NGF/Tirofiban nerve conduits

et al. 2011

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“…When combined with biomaterials and with controlled release, its effect can be strengthened (79)(80)(81) . The ability of the NGF to promote functional recovery after lesions was confirmed in experimental studies (65,81) . The endogenous brain-derived neurotrophic factor (BDNF) demonstrates an important role in the induction of the cell body response in injured rat neurons.…”

Section: Growth Factors In Nerve Repairmentioning

confidence: 64%

“…The complexity of nerve regeneration involves a range of elements that interact with one another, and are all essential to the process; among them, the growth factors (GF) aroused a great deal of interest in the scientific community (62) , due to their performance as important cell modulators (62)(63)(64)(65)(66)(67)(68)(69)(70)(71)(72)(73)(74)(75)(76)(77)(78) (Table 2). Degenerated peripheral nerves are an important source of these factors, as are the SCs.…”

Section: Growth Factors In Nerve Repairmentioning

confidence: 99%

Regeneração de nervos periféricos: terapia celular e fatores neurotróficos

Sebben¹,

Lichtenfels²,

Silva

2011

Rev. bras. ortop.

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Peripheral nerve trauma results in functional loss in the innervated organ, and recovery without surgical intervention is rare. Many surgical techniques can be used for nerve repair. Among these, the tubulization technique can be highlighted: this allows regenerative factors to be introduced into the chamber. Cell therapy and tissue engineering have arisen as an alternative for stimulating and aiding peripheral nerve regeneration. Therefore, the aim of this review was to provide a survey and analysis on the results from experimental and clinical studies that used cell therapy and tissue engineering as tools for optimizing the regeneration process. The articles used came from the LILACS, Medline and SciELO scientific databases. Articles on the use of stem cells, Schwann cells, growth factors, collagen, laminin and platelet-rich plasma for peripheral nerve repair were summarized over the course Rev Bras Ortop. 2011;46(6):643-49 of the review. Based on these studies, it could be concluded that the use of stem cells derived from different sources presents promising results relating to nerve regeneration, because these cells have a capacity for neuronal differentiation, thus demonstrating effective functional results. The use of tubes containing bioactive elements with controlled release also optimizes the nerve repair, thus promoting greater myelination and axonal growth of peripheral nerves. Another promising treatment is the use of platelet-rich plasma, which not only releases growth factors that are important in nerve repair, but also serves as a carrier for exogenous factors, thereby stimulating the proliferation of specific cells for peripheral nerve repair.

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“…It is vital for the development and survival of both sensory and sympathetic neurons; in its absence these undergo apoptosis [2]. In rats, the application of NGF has been shown to promote sciatic nerve regeneration after a crush injury [3]. NGF may act as an inflammatory mediator [4] and as a mediator in asthma.…”

Section: Introductionmentioning

confidence: 99%

Label‐Free Impedimetric Immunosensor for Nerve Growth Factor Protein Constructed Using an Automated Dispensing System

et al. 2013

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We describe the development, validation and optimisation of label-free immunosensors for Nerve Growth Factor (NGF) protein. Screen-printed carbon electrodes were electrochemically coated with polyaniline, and onto this antibodies to NGF were immobilised using electrostatic or neutravidin-biotin interactions. These were constructed manually or using an automated liquid dispensing system. QCM demonstrated deposition of individual layer stages and binding of NGF. Studies revealed clear increases in immunosensor impedance following antigen exposure. Automated methodologies produced sensors with lowered sample-to-sample variation. Affinity produced electrodes offered higher sensitivities than those produced utilising electrostatic interactions. NGF between 25-1000 pg mL À1 could be determined using the optimised sensor.

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The effect of collagen-binding NGF-β on the promotion of sciatic nerve regeneration in a rat sciatic nerve crush injury model

Cited by 117 publications

References 37 publications

Promoting regeneration of peripheral nerves in-vivo using new PCL-NGF/Tirofiban nerve conduits

Promoting regeneration of peripheral nerves in-vivo using new PCL-NGF/Tirofiban nerve conduits

Regeneração de nervos periféricos: terapia celular e fatores neurotróficos

Label‐Free Impedimetric Immunosensor for Nerve Growth Factor Protein Constructed Using an Automated Dispensing System

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