The effect of colchicine on alveolar bone loss in ligature-induced periodontitis

Abstract

Colchicine is widely used in the treatment of several inflammatory diseases due to its anti-inflammatory effect, but effects on bone metabolism are unclear. The aim of this study was to evaluate the effects of systemically-administered colchicine on healthy periodontium and experimentally-induced periodontitis. In total, 42 male Wistar rats were included in this study. A non-ligated group constituting the negative control group (Control, C, n = 6) and a ligature-only group forming the positive control group (LO, n = 12) were created separately. Twelve rats were treated with 0.4 mg/kg colchicine and another 12 with 1 mg/kg colchicine. In the colchicine-administered groups, right mandibles constituted the ligated groups (1 mgC-L or 0.4 mgC-L) and left mandibles formed the corresponding non-ligated controls (1mgC or 0.4mgC). Silk ligatures were placed at the gingival margin of the lower first molars. The animals were euthanized at different time-points of healing (11 or 30 days). Alveolar bone loss was clinically measured and TRAP+ osteoclasts, osteoblastic activity, and MMP-1 expression were examined histologically. There was no increase in alveolar bone loss with either colchicine dose in healthy periodontium (p > 0.05) and the highest level of alveolar bone loss, TRAP+ osteoclast number, and MMP-1 expression were measured in the LO group (p < 0.05). The 0.4 mgC-L group showed less alveolar bone loss at 11 days (p < 0.05), but greater loss at 30 days. The 1 mgC-L group showed higher osteoblast number than the other ligated groups (p < 0.05) at both time-points. In summary, colchicine did not increase alveolar bone loss in healthy periodontium and also may tend to reduce periodontitis progression. However, further extensive study is necessary to understand the mechanism of colchicine action on alveolar bone loss in periodontitis.

Ultrastructural Study of the Effect of Curcumin Nanoparticles Administration on the Alveolar Bone Structure in Rats With Induced Periodontitis

“…Induction of periodontitis in the current study was performed by using 4-0 silk ligature for 2 weeks. This is in accordance to Moraes et al (2020) (25) and other researchers (26,27) who used silk ligatures to induce experimental periodontitis because it is a simple, effective, cheap and an available method. In addition, Kurt et al (14) proved that tying silk ligatures around the experimental rats' mandibular first molars for 2 weeks was capable to create an experimental periodontitis model.…”

Anti‑gouty arthritis and anti‑hyperuricemia properties of celery seed extracts in rodent models

“…The working concentrations of FBX and COL were selected according to previous studies (39,40). Celery seed treatment doses were selected following preliminary experiments (data not shown).…”

Interleukin‐35 inhibits alveolar bone resorption by modulating the Th17/Treg imbalance during periodontitis

“…Several strategies have been assayed in order to modulate the Th17/Treg imbalance during periodontitis and arrest the alveolar bone loss, such as subcutaneous vaccination with Porphyromonas gingivalis , gastric feeding of colchicine, and oral administration of retinoic acid, tamibarotene or oleuropein, with promising results (Jin, Wang, Liu, & Lin, 2014; Taskan, Balci Yuce, Karatas, Gevrek, & Toker, 2019; Toker, Yuce, Yildirim, Tekin, & Gevrek, 2019; Wang, Guan, Jin, Lin, & Gao, 2015; Wang, Wang, Jin, Gao, & Lin, 2014). To the best of the authors' knowledge, this study provides the first evidence that IL‐35 administration suppresses periodontitis.…”