The effect of CO donor hemin on the antioxidant and osmoprotective systems state in Arabidopsis of a wild-type and mutants defective in jasmonate signaling under salt stress

Abstract

The role of the gasotransmitter carbon monoxide (Co) in signaling and adaptive processes in plants has been studied insufficiently. There are indirect data indicating jasmonate signaling participation in realization of CO effects, but the possible connection between carbon monoxide and jasmonate signaling during plant adaptation to salt stress remains unclear. We studied the carbon monoxide donor hemin effect on the Arabidopsis of a wild-type (Col-0) and defective in jasmonate signaling coi1 and jin1 mutants response to the salt stress. Arabidopsis thaliana 4-week-old plants were grown on a modified Hoagland's medium. plants were incubated for 24 h in usual or 2 µM hemin containing culture medium, then transferred to 150 mM NaCl containing media and incubated for 24 h before the medium was replaced with the usual one. It was shown that salt stress caused water deficiency and superoxide dismutase and catalase activity decrease in the plants of all three genotypes. Treatment with 2 μM hemin stabilized the levels of catalase activity and photosynthetic pigments and increased guaiacol peroxidase activity in a wild-type, but not in coi1 and jin1 mutant plants after stress induction. Treated with hemin wild-type Arabidopsis plants accumulated more proline and sugars in response to stress than treated coi1 and jin1 mutants. It was concluded that jasmonate signaling can be involved in adaptive processes induced by exogenous carbon monoxide. K e y w o r d s: carbon monoxide, jasmonate signaling, salt resistance, wild type Arabidopsis thaliana, coi1 and jin1 mutants, antioxidant enzymes, pigments, proline.

Metabolic Adaptations in Rapeseed: Hemin-Induced Resilience to NaCl Stress by Enhancing Growth, Photosynthesis, and Cellular Defense Ability

“…Hemin soaking can effectively improve the soluble protein content of the two rape varieties and help the cells absorb water in the external environment, thus reducing the damage caused by NaCl stress. This confirms that hemin enhances the osmoprotector [ 59 ], thereby inhibiting oxidative-damage-induced growth by removing excess ROS and REDOX homeostasis from the electron transport chain.…”

Carbon Monoxide Alleviates Salt-Induced Oxidative Damage in Sorghum bicolor by Inducing the Expression of Proline Biosynthesis and Antioxidant Genes

“…A further increase in proline content was observed in the presence of CO (1 and 1.5 μM of CO) under salt stress compared to seedlings treated with salt only ( Figure 3 B). Pretreatment with hemin under salt stress conditions has been reported to enhance proline accumulation in wild-type Arabidopsis leaves [ 61 ]. The increase in proline content induced by exogenous application of CO is linked to the activation of pyrroline-5-carboxylate synthase (P5CS) and a decrease in proline dehydrogenase (ProDH) activity, thereby alleviating salt-induced damage [ 64 ].…”

Signal Mediators in the Implementation of Jasmonic Acid’s Protective Effect on Plants under Abiotic Stresses

“…At the same time, coi1 and jin1 mutants did not exhibit any of these effects under the influence of the CO donor. Thus, the results obtained indicate the involvement of components of jasmonate signaling in the stress-protective effects of exogenous carbon monoxide [ 184 ] ( Figure 4 ). To elucidate the mechanisms underlying the involvement of jasmonate signaling proteins in the implementation of the described CO effects, special studies are needed.…”

“…It is possible that the role of COI1 and JIN1/MYC2 proteins in the action of exogenous CO is due to the ability of this gasotransmitter to induce JA synthesis. Evidence has also been obtained regarding the role of jasmonate signaling in carbon monoxide-induced protective responses of Arabidopsis plants to salt stress [184]. Arabidopsis plants of the wild-type and jasmonate signaling mutants, coi1 and jin1, were used in this study.…”

“…Evidence has also been obtained regarding the role of jasmonate signaling in carbon monoxide-induced protective responses of Arabidopsis plants to salt stress [ 184 ]. Arabidopsis plants of the wild-type and jasmonate signaling mutants, coi1 and jin1 , were used in this study.…”