The Effect of Chronic Infection With Aspergillus fumigatus on Lung Function and Hospitalization in Patients With Cystic Fibrosis

Amin, Reshma; Dupuis, Annie; Aaron, Shawn D.; Ratjen, Félix

doi:10.1378/chest.09-1103

Cited by 326 publications

(316 citation statements)

References 22 publications

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“…In contrast, it seems more limited in CF, with a unique case of respiratory infections caused by this species complex having been reported for this genetically inherited disease (14). Nevertheless, the present identification of some genotypes chronically colonizing the lower airways of patients with CF demonstrates the persisPatient P17 -sampling date 1 9 9 6 1 9 9 7 / 0 9 / 2 5 2 0 0 1 1 9 9 8 / 0 1 / 2 1 1 9 9 7 / 1 2 / 1 1 1 9 9 7 / 1 0 / 2 7 tence of these usually saprophytic fungi in the respiratory tract and their multiplication, suggesting their contribution to the inflammatory reaction which progressively leads to clinical and functional deterioration, as demonstrated for long-term colonization of the airways by Aspergillus fumigatus or Candida albicans (32,33,34). Among the 116 isolates studied here, 29 genotypes were identified.…”

Section: Discussionmentioning

confidence: 99%

Long-Term Rasamsonia argillacea Complex Species Colonization Revealed by PCR Amplification of Repetitive DNA Sequences in Cystic Fibrosis Patients

et al. 2016

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The aim of this work was to document molecular epidemiology of Rasamsonia argillacea species complex isolates from cystic fibrosis (CF) patients. In this work, 116 isolates belonging to this species complex and collected from 26 CF patients and one patient with chronic granulomatous disease were characterized using PCR amplification assays of repetitive DNA sequences and electrophoretic separation of amplicons (rep-PCR). Data revealed a clustering consistent with molecular species identification. A single species was recovered from most patients. Rasamsonia aegroticola was the most common species, followed by R. argillacea sensu stricto and R. piperina, while R. eburnea was not identified. Of 29 genotypes, 7 were shared by distinct patients while 22 were patient specific. In each clinical sample, most isolates exhibited an identical genotype. Genotyping of isolates recovered from sequential samples from the same patient confirmed the capability of R. aegroticola and R. argillacea isolates to chronically colonize the airways. A unique genotype was recovered from two siblings during a 6-month period. In the other cases, a largely dominant genotype was detected. Present results which support the use of rep-PCR for both identification and genotyping for the R. argillacea species complex provide the first molecular evidence of chronic airway colonization by these fungi in CF patients.R asamsonia argillacea, which was first reported in clinical practice as a Penicillium species, then reclassified in the genus Geosmithia, and finally reassigned in 2013 to the new genus Rasamsonia, is now considered an emerging pathogen (1). Since its first report in humans as Penicillium emersonii (2), there has been an increasing number of cases reported in both dogs (3, 4, 5) and humans (6). Nevertheless, it is likely that the number of human infections caused by this fungus has long been underestimated because of the lack of specificity of its morphological features and to subsequent misidentifications with some Penicillium or Paecilomyces species.Only six species were previously recognized in the genus Rasamsonia described in 2012, i.e., R. argillacea, R. composticola, R. brevistipitata, R. byssochlamydoides, R. cylindrospora, and R. emersonii (7). However, multilocus sequence analysis of several R. argillacea isolates revealed a clustering which superimposed some phenotypic differences. Therefore, R. argillacea is now considered a species complex comprising four distinct species: R. argillacea sensu stricto, R. piperina, R. aegroticola, and R. eburnea (8).Infections caused by species of the R. argillacea complex have been reported in various clinical contexts in human. Chronic granulomatous disease (CGD) (9, 10, 11) and cystic fibrosis (CF) (12,13,14,15,16,17) are the major underlying clinical conditions. Rasamsonia infections may also occur in bone marrow transplant recipients (18, 19) but also may occur in the absence of any predisposing factors, as evidenced by the pulmonary and aortic graft infection reported by Doyon et al. (...

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Section: Discussionmentioning

confidence: 99%

Long-Term Rasamsonia argillacea Complex Species Colonization Revealed by PCR Amplification of Repetitive DNA Sequences in Cystic Fibrosis Patients

et al. 2016

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“…The rate of sputum positivity in patients with CF is high, but only a fraction of patients develop the wellestablished clinical manifestation of allergic bronchopulmonary aspergillosis (ABPA). Whether the detection of A. fumigatus in CF airways is clinically relevant in patients without ABPA is less clear, although pulmonary exacerbation rates are higher in patients chronically positive for A. fumigatus (10). Studies provide further support for the concept that A. fumigatus may not be an innocent bystander in CF lung disease.…”

Section: Cystic Fibrosis Microbiologymentioning

confidence: 99%

Update in Cystic Fibrosis 2012

Goss

Ratjen

2013

Am J Respir Crit Care Med

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“…Increasing attention has focused on the relationship between dominant bacterial and fungal species associated with chronic endobronchial infection in CF, particularly P. aeruginosa (Pa) and Af. Clinical studies suggest that co-infection with Pa and Af is common and associated with worsened status and prognosis (29)(30)(31) (34), and that as Pa adapts over time to the CF airway environment during chronic infection, eg producing mucoid and small colony Pa persister phenotypes (35), it becomes less inhibitory (33). Perhaps these two dominant CF pathogens adapt to mutually beneficial states that might explain why effective anti-Pa antibiotic therapy also reduces in vivo Af burden (36).…”

Section: Univ Of Iowa Howard Hughes Medical Institute Iowa City Iamentioning

confidence: 99%

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2017

Pediatric Pulmonology

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The Effect of Chronic Infection With Aspergillus fumigatus on Lung Function and Hospitalization in Patients With Cystic Fibrosis

Cited by 326 publications

References 22 publications

Long-Term Rasamsonia argillacea Complex Species Colonization Revealed by PCR Amplification of Repetitive DNA Sequences in Cystic Fibrosis Patients

Long-Term Rasamsonia argillacea Complex Species Colonization Revealed by PCR Amplification of Repetitive DNA Sequences in Cystic Fibrosis Patients

Update in Cystic Fibrosis 2012

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