The Effect of Chronic Immunosuppressive Regimens Treatment on Aortal Media Morphology and the Balance between Matrix Metalloproteinases (mmp-2 and mmp-9) and Their Inhibitors in the Abdominal Aorta of Rats

Surówka, Anna; Szumilas, Kamila; Wilk, Aleksandra; Misiakiewicz-Has, Kamila; Ciechanowski, Kazimierz; Kędzierska-Kapuza, Karolina

doi:10.3390/ijerph19116399

Cited by 6 publications

(9 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MMP-9 is elevated in patients with acute lung injury (ALI) and acute respiratory distress syndrome (ARDS), which correlates closely with lung injury severity [ 19 , 20 , 21 ] and supports the assertion that MMP-9 plays a major role in the pathophysiology of sepsis [ 10 , 17 , 22 ]. A single-nucleotide polymorphism (SNP) at position-1562 (C/T) in the MMP-9 gene has been associated with differential MMP-9 expression and increased mortality in sepsis patients [ 23 ].…”

Section: Introductionmentioning

confidence: 71%

Paper-Based Biosensor for the Detection of Sepsis Using MMP-9 Biomarker in FIP Mice Model

Alekhmimi,

Raddadi,

Alabdulwahed

et al. 2023

Biosensors

View full text Add to dashboard Cite

Sepsis is an immune response to a microbial invasion that causes organ injury and dysfunction due to a systemic inflammatory response. Sepsis is a serious, life-threatening condition and a widely recognized global health challenge. Given its high death rate, it is critical to diagnose sepsis and start treatment as early as possible. There is an urgent need for a sensitive and rapid screening method for detecting sepsis. In this study, we investigated the use of MMP-9 as a biomarker for sepsis. A colorimetric paper-based biosensor was used for the detection of MMP-9 utilizing peptide-magnetic nanoparticle conjugates. The method is based on the cleavage of the MMP-9-specific peptide by the protease leading to the detaching of the magnetic beads from the sensor surface and changing of color. A fecal intraperitoneal (FIP) challenge was used to induce sepsis in mice, and an MMP-9 secretion was measured by taking blood and Bronchoalveolar Lavage (BAL) fluid samples at 1 h, 2 h, 4 h, and 20 h (early sepsis) post-challenge intervals. The results of the paper-based sensor for the detection of MMP-9 levels in blood samples and BAL samples were compared with ELISA and Western Blot. We found that both blood and BAL levels of MMP-9 increased immediately and could be detected as early as 1 h in FIP mice post-challenge. Our work adds evidence to the assertion that MMP-9 is a reliable biomarker for the detection of sepsis at early stages.

show abstract

Section: Introductionmentioning

confidence: 71%

Paper-Based Biosensor for the Detection of Sepsis Using MMP-9 Biomarker in FIP Mice Model

Alekhmimi,

Raddadi,

Alabdulwahed

et al. 2023

Biosensors

View full text Add to dashboard Cite

show abstract

“…These changes included the thickness of the tunic media, wider distances between elastic lamellae, an increase in the number of vascular smooth muscle cells, and changes in collagen deposition. The authors suggested that this observation was associated with MMP-2, MMP-9/TIMP-2, and TIMP-1 imbalances, which was also determined and noticed [ 52 ].…”

Section: Classes Of Immunosuppressive Drugsmentioning

confidence: 82%

“…Additionally, arteries of animals that were treated with cyclosporine indicated spot thickening of the endothelium connected with the increased depositing of extracellular matrix components, which led to narrowed vessel lumen [ 51 ]. Surówka et al [ 52 ] examined rats aortas treated with different immunosuppressive drugs. The most profound alterations regarding vessels were observed in calcineurin inhibitor-based protocols.…”

Section: Classes Of Immunosuppressive Drugsmentioning

confidence: 99%

“…Wang et al [ 67 ] suggest that glycyrrihizic acid and prednisone improve renal function, relieve the renal morphological changes, and decrease interstitial fibrosis in rats [ 67 ]. However, some researchers, based on the examination of immunosuppressive regimens, where prednisone was included, indicated the severe effect of these drugs on various tissues, including kidney [ 46 , 47 , 52 ]. Nevertheless, these studies concerned triple-drug protocols; therefore, the role of prednisone on organs was not clear [ 46 , 47 , 52 ].…”

Section: Classes Of Immunosuppressive Drugsmentioning

confidence: 99%

See 1 more Smart Citation

Current Status Regarding Immunosuppressive Treatment in Patients after Renal Transplantation

Szumilas

Wilk

Wiśniewski

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

Renal transplantation is now the best treatment for end-stage renal failure. To avoid rejection and prolong graft function, organ recipients need immunosuppressive therapy. The immunosuppressive drugs used depends on many factors, including time since transplantation (induction or maintenance), aetiology of the disease, and/or condition of the graft. Immunosuppressive treatment needs to be personalised, and hospitals and clinics have differing protocols and preparations depending on experience. Renal transplant recipient maintenance treatment is mostly based on triple-drug therapy containing calcineurin inhibitors, corticosteroids, and antiproliferative drugs. In addition to the desired effect, the use of immunosuppressive drugs carries risks of certain side effects. Therefore, new immunosuppressive drugs and immunosuppressive protocols are being sought that exert fewer side effects, which could maximise efficacy and reduce toxicity and, in this way, reduce both morbidity and mortality, as well as increase opportunities to modify individual immunosuppression for renal recipients of all ages. The aim of the current review is to describe the classes of immunosuppressive drugs and their mode of action, which are divided by induction and maintenance treatment. An additional aspect of the current review is a description of immune system activity modulation by the drugs used in renal transplant recipients. Complications associated with the use of immunosuppressive drugs and other immunosuppressive treatment options used in kidney transplant recipients have also been described.

show abstract

“…Tissue integrity and homeostasis is maintained by the balance and dynamics with which the ECM interacts with cells. Disturbances in this process can lead to the development of pathological cardiac and vascular changes, such as aneurysms, chronic venous insufficiency, atherosclerosis, coronary artery disease, myocarditis, heart failure, endocarditis and cardiomyopathies [ 28 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 ].…”

Section: Metalloproteinasesmentioning

confidence: 99%

The Effect of Calcineurin Inhibitors on MMPs Activity in Heart and Their Side Effects—A Review of Literature

Surówka,

Prowans,

Żołnierczuk

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

This review focuses on the role of metalloproteinases in the pathogenesis of myocardial injury in various disease entities. It reveals how the expression and serum levels of metalloproteinases and their inhibitors change in many disease states. At the same time, the study offers a review of the impact of immunosuppressive treatment on this relationship. Modern immunosuppressive treatment is based mainly on the use of calcineurin inhibitors, including cyclosporine A and tacrolimus. The use of these drugs may carry a number of side effects, specifically to the cardiovascular system. The scale and degree of long-term influence on the organism remains unclear, but a significant risk of complications for transplant recipients who take immunosuppressive drugs as part of their daily treatment is to be expected. Therefore, the knowledge on this subject should be expanded and the negative effects of post-transplant therapy minimized. Immunosuppressive therapy plays an important role in the expression and activation of tissue metalloproteinases and their specific inhibitors, which leads to many tissue changes. The presented study is a collection of research results on the effects of calcineurin inhibitors on the heart, with particular emphasis placed on the participation of MMP-2 and MMP-9. It is also an analysis of the effects of specific heart diseases on myocardial remodeling through inductive or inhibitory effects on matrix metalloproteinases and their inhibitors.

show abstract

The Effect of Chronic Immunosuppressive Regimens Treatment on Aortal Media Morphology and the Balance between Matrix Metalloproteinases (mmp-2 and mmp-9) and Their Inhibitors in the Abdominal Aorta of Rats

Cited by 6 publications

References 57 publications

Paper-Based Biosensor for the Detection of Sepsis Using MMP-9 Biomarker in FIP Mice Model

Paper-Based Biosensor for the Detection of Sepsis Using MMP-9 Biomarker in FIP Mice Model

Current Status Regarding Immunosuppressive Treatment in Patients after Renal Transplantation

The Effect of Calcineurin Inhibitors on MMPs Activity in Heart and Their Side Effects—A Review of Literature

Contact Info

Product

Resources

About