The effect of chorionic villus sampling on the number of fetal cells isolated from maternal blood and on maternal serum alpha-fetoprotein levels

Jansen, Mieke; Brandenburg, Helen; Wildschut, Hajo I. J.; Martens, Anton C.; Hagenaars, A. M.; Wladimiroff, J. W.; Veld, Peter A. In ‘t

doi:10.1002/(sici)1097-0223(199710)17:10<953::aid-pd182>3.0.co;2-e

Cited by 25 publications

(13 citation statements)

References 24 publications

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“…Factors that may in¯uence these frequencies include the type of fetal cell analysed, gestational age at the time of sampling and the accuracy of methods to enrich, identify, and quantify the fetal target population. The incidence of fetal cells in maternal blood has been reported to increase after chorionic villus sampling (Jansen et al, 1997), in patients with pre-eclampsia (Holzgreve et al, 1998;Lo et al, 1999;Jansen et al, submitted), and in pregnancies in which the fetal and placental karyotype was abnormal (Elias et al, 1992;Ganshirt-Ahlert et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

How useful is the in vitro expansion of fetal CD34+ progenitor cells from maternal blood samples for diagnostic purposes?

Jansen,

Korver‐Hakkennes,

van Leenen

et al. 2000

Prenat. Diagn.

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Fetal cells present in the maternal circulation are a potential source of fetal DNA that can be used for the development of a prenatal diagnostic test. Since their numbers are very low, amplification of fetal cells has been discussed for a long time. So far, most studies have focused on culturing fetal erythroid cells. In this study, we evaluated whether limiting numbers of fetal haemopoietic progenitor cells present in an excess of maternal cells were able to overgrow the maternal component. Therefore, we used a model system in which limiting numbers of male CD34+ umbilical cord blood cells were diluted in 400 000 female CD34+ peripheral blood cells. The number of XY positive cells derived from umbilical cord blood was determined using two-colour in situ hybridization with X and Y chromosomal probes. We demonstrated a 1500-fold relative expansion of male umbilical cord blood cells over the peripheral blood component after three weeks of liquid culture, which also corresponded to the extent of expansion of CD34+ cells derived from 20-week fetal blood. However, application of the same culture protocol to maternal blood samples obtained at 7-16 weeks of gestation showed no preferential growth of fetal haemopoietic progenitor cells. This study, therefore, suggests that fetal primitive haemopoietic progenitor cells do either not circulate in maternal blood before 16 weeks of gestation, or require different combinations/concentrations of cytokines for their in vitro expansion.

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Section: Introductionmentioning

confidence: 99%

How useful is the in vitro expansion of fetal CD34+ progenitor cells from maternal blood samples for diagnostic purposes?

Jansen,

Korver‐Hakkennes,

van Leenen

et al. 2000

Prenat. Diagn.

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“…Another factor that in¯uences the incidence of fetal cells in maternal blood is CVS, which has previously been reported to cause a fetomaternal transfusion of fetal NRBCs (Jansen et al, 1997). The question arises as to whether it may also have an effect on the number of fetal haemopoietic progenitor cells in the maternal circulation.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

How useful is thein vitro expansion of fetal CD34+ progenitor cells from maternal blood samples for diagnostic purposes?

et al. 2000

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Fetal cells present in the maternal circulation are a potential source of fetal DNA that can be used for the development of a prenatal diagnostic test. Since their numbers are very low, amplification of fetal cells has been discussed for a long time. So far, most studies have focused on culturing fetal erythroid cells. In this study, we evaluated whether limiting numbers of fetal haemopoietic progenitor cells present in an excess of maternal cells were able to overgrow the maternal component. Therefore, we used a model system in which limiting numbers of male CD34+ umbilical cord blood cells were diluted in 400 000 female CD34+ peripheral blood cells. The number of XY positive cells derived from umbilical cord blood was determined using two‐colour in situ hybridization with X and Y chromosomal probes. We demonstrated a 1500‐fold relative expansion of male umbilical cord blood cells over the peripheral blood component after three weeks of liquid culture, which also corresponded to the extent of expansion of CD34+ cells derived from 20‐week fetal blood. However, application of the same culture protocol to maternal blood samples obtained at 7–16 weeks of gestation showed no preferential growth of fetal haemopoietic progenitor cells. This study, therefore, suggests that fetal primitive haemopoietic progenitor cells do either not circulate in maternal blood before 16 weeks of gestation, or require different combinations/concentrations of cytokines for their in vitro expansion. Copyright © 2000 John Wiley & Sons, Ltd.

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“…42 The number of NRBC sorted from maternal blood and stained with anti-epsilon and antigamma globin antibodies increases significantly after a CVS has been performed. The potential for these invasive procedures to cause rhesus isoimmunisation is well known.…”

Section: Cell Numbersmentioning

confidence: 99%

“…104 Of the truly trisomic pregnancies, 11-13 % of the cell nuclei scored had only 2 signals. 108−110 Factors influencing the hybridisation efficiency of FISH probes include the method of cell preparation and fixation 9,10,42,111 the type of anticoagulant used, 109 the method of fetal cell sorting 112 and the inherent properties of the FISH probes themselves. Unfortunately, very few cell sorting systems have produced yields even approaching 30-50 cells.…”

Section: Cell Analysismentioning

confidence: 99%

Harvesting Fetal Cells From the Maternal Circulation

McEwan

2005

Fet. Matern. Med. Rev.

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Schmorl is generally credited with being the first to report finding fetal cells in maternal blood. In 1893 he identified trophoblasts in the pulmonary circulation of women who had died of eclampsia. Years later, in 1969, Walnowska identified Y chromosomes in lymphocytes isolated from the blood of pregnant women carrying male fetuses and this was repeated by Herzenberg in 1979 in white blood cells recognised as fetal by their surface HLA-A2 expression. Other sporadic reports followed but not until the 1990's did investigation into harvesting fetal cells from maternal blood begin in earnest. The aim of this article is to review the progress made in isolating and analysing these cells for the purposes of prenatal diagnosis.

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The effect of chorionic villus sampling on the number of fetal cells isolated from maternal blood and on maternal serum alpha-fetoprotein levels

Cited by 25 publications

References 24 publications

How useful is the in vitro expansion of fetal CD34+ progenitor cells from maternal blood samples for diagnostic purposes?

How useful is the in vitro expansion of fetal CD34+ progenitor cells from maternal blood samples for diagnostic purposes?

How useful is thein vitro expansion of fetal CD34+ progenitor cells from maternal blood samples for diagnostic purposes?

Harvesting Fetal Cells From the Maternal Circulation

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